{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T14:23:39Z","timestamp":1747232619237,"version":"3.40.5"},"reference-count":19,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"4","license":[{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["biomedica"],"abstract":"<jats:p>Introducci\u00f3n. En Nicaragua es necesario estandarizar pruebas moleculares como la PCR en tiempo real (quantitative Polymerase Chain Reaction, qPCR) que mejoren el diagn\u00f3stico de leptospirosis en humanos y animales.Objetivo. Evaluar tres qPCR para la detecci\u00f3n de leptospiras pat\u00f3genas en animales dom\u00e9sticos de Nicaragua.Materiales y m\u00e9todos. Se dise\u00f1aron cebadores para la amplificaci\u00f3n del gen LipL32 en SYBR Green (SYBR Green-A) y TaqMan, y en otros descritos previamente (SYBR Green-B). Las secuencias de 12 cepas obtenidas de la base de datos del National Center for Biotechnology Information (NCBI) se alinearon para la b\u00fasqueda de sondas y cebadores. La sensibilidad anal\u00edtica se determin\u00f3 calculando el equivalente gen\u00f3mico detectable, se utilizaron 18 cepas de referencia para la sensibilidad diagn\u00f3stica y 28 controles negativos para la especificidad. Los m\u00e9todos se aplicaron en 129 muestras de orina de animales dom\u00e9sticos.Resultados. En SYBR Green-A se obtuvo un l\u00edmite de detecci\u00f3n de cuatro equivalentes gen\u00f3micos; en TaqMan, la sensibilidad fue del 94,4 % (IC95% 81,1-100,0). Con SYBR Green-A, se obtuvo una sensibilidad del 77,8 % (IC95% 55,8-99,8), en tanto que con SYBR Green-B fue del 61,1 % (IC95% 35,8-86,4). En las tres pruebas se logr\u00f3 una especificidad del 100 % (IC95% 98,2-100,0). El 26,4 % de las muestras de animales dom\u00e9sticos fueron positivas con SYBR Green-A y el 6,2 % con SYBR Green-B.Conclusiones. El SYBR Green-A present\u00f3 un l\u00edmite de detecci\u00f3n bajo, en tanto que las tres t\u00e9cnicas evaluadas mostraron alta especificidad, en tanto que la TaqMan tuvo la mayor sensibilidad.<\/jats:p>","DOI":"10.7705\/biomedica.5170","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T21:00:38Z","timestamp":1607029238000},"page":"673-681","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Evaluaci\u00f3n de tres PCR cuantitativas para la detecci\u00f3n de leptospiras pat\u00f3genas en animales dom\u00e9sticos en Nicaragua"],"prefix":"10.7705","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1932-3227","authenticated-orcid":false,"given":"Byron","family":"Flores","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4121-8087","authenticated-orcid":false,"given":"Nabil","family":"Halaihel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2125-6016","authenticated-orcid":false,"given":"Tania","family":"P\u00e9rez-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7370-5763","authenticated-orcid":false,"given":"Jessica","family":"Sheleby-El\u00edas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2042-927X","authenticated-orcid":false,"given":"Brenda","family":"Mora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1690-5034","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Fuertes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5778-5721","authenticated-orcid":false,"given":"William","family":"Jir\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"key":"101891","doi-asserted-by":"crossref","unstructured":"Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Chierakul W, Limmathurotsakul D, et al. 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