{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:48:37Z","timestamp":1740167317080,"version":"3.37.3"},"reference-count":23,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"3","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006434","name":"Consejo de Desarrollo Cient\u00edfico y Human\u00edstico, Universidad Central de Venezuela","doi-asserted-by":"publisher","award":["PG: 09-8760-2013"],"award-info":[{"award-number":["PG: 09-8760-2013"]}],"id":[{"id":"10.13039\/501100006434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["biomedica"],"abstract":"<jats:p>Introduction: The thymus is active mainly during the neonatal and pre-adolescent periods.Objective: To test na\u00efve thymocytes proliferation and monocytes stimulation.Materials and methods: We collected fresh thymus tissue from neonate mice after surgery. Suspension cells were coated onto Ficoll-Hypaque support. The obtained cells (thymocytes) were cultured measuring the proliferation of na\u00efve T cells stimulated by Crotalus durissus cumanensis (Cdc) venom at sub-lethal doses (20 ng). Then, we supplemented the wells with AlamarBlue\u2122 and incubated them for 5 h to test their proliferation. Mononuclear cells from mice peripheral blood were collected and layered onto the support of the Ficoll-Hypaque solution. We added the thymocytes actively dividing (25 x 105 cells) from cultures stimulated with Cdc venom at 20 ng\/well to cultured monocytes freshly obtained from the Ficoll-Hypaque separation. Both cell populations were incubated for 36 h until monocytes matured to macrophages.Results: The na\u00efve thymocytes rapidly proliferated after stimulation with the Cdc venom (NTCdc) and these successively induced the maturation and function of monocytes progenitor cells to mature macrophages, which ingested Chinese ink.Conclusions: The na\u00efve thymocytes proliferated by stimulation with the Cdc venom and subsequently the NT\/Cdc induced the rapid maturation and function of monocytes progenitor cells becoming mature macrophages with their phenotypic characteristics.<\/jats:p>","DOI":"10.7705\/biomedica.5599","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T16:26:36Z","timestamp":1632500796000},"page":"449-457","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":1,"title":["A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom"],"prefix":"10.7705","volume":"41","author":[{"given":"Mar\u00eda M.","family":"Pulido-M\u00e9ndez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvia","family":"Azuaje","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1234-7522","authenticated-orcid":false,"given":"Alexis","family":"Rodr\u00edguez-Acosta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"105234","doi-asserted-by":"crossref","unstructured":"Markert ML, Devlin BH, Chinn IK, McCarthy EA. Thymus transplantation in complete DiGeorge anomaly. Immunol Res. 2009;44:61-70. https:\/\/doi.org\/10.1007\/s12026-008-8082-5","DOI":"10.1007\/s12026-008-8082-5"},{"key":"105235","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Cruz S, Garc\u00eda-Jim\u00e9nez R, Zucatelli-Mendonc A, Petricevich VL. Pro- and antiinflammatory cytokines release in mice injected with Crotalus durissus terrificus venom. Mediators Inflamm. 2008;2008:874962. https:\/\/doi.org\/10.1155\/2008\/874962","DOI":"10.1155\/2008\/874962"},{"key":"105236","doi-asserted-by":"crossref","unstructured":"Holland AM, van den Brink MR. Rejuvenation of the aging T cell compartment. Curr Opin Immunol. 2009;21:454-9. https:\/\/doi.org\/10.1016\/j.coi.2009.06.002","DOI":"10.1016\/j.coi.2009.06.002"},{"key":"105237","doi-asserted-by":"crossref","unstructured":"van den Broek T, Borghans JAM, van Wijk F. The full spectrum of human naive T cells. 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