{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T14:23:56Z","timestamp":1747232636131,"version":"3.40.5"},"reference-count":38,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"4","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["biomedica"],"abstract":"<jats:p>Introduction: Anisakiasis is a human parasitic disease caused by the consumption of raw fish or shellfish containing larvae of the Anisakidae family. It is currently considered an emerging disease of public health interest.Objective: To identify the presence of larvae of the Anisakidae family in samples of frozen raw fish fillets intended for human consumption in markets in Medell\u00edn and its metropolitan area in Antioquia, Colombia.Materials and methods: A cross-sectional study was carried out, in which larvae of the Anisakidae family were detected and identified in frozen raw fish fillets from three representative markets in Medell\u00edn and its metropolitan area. A total of 384 ready for consumption fillets were analyzed (197 sawfish, 137 salmon, 37 tuna, and 13 hake), using the pressing and ultraviolet light method. Taxonomic keys were used to identify the collected parasites and to establish its genus. Conventional PCR and Sanger sequencing was performed to determine the species.Results: Four larvae were found in 4 of the 384 (1.04%) fillets (CI95% 1.04 \u00b1 1.01%). The species of fish in which the larvae were found was sawfish (Scomberomorus spp.) and the genus and species of the larvae was established as Anisakis pegreffii.Conclusions: According to the study, the presence of Anisakis parasites in frozen raw fish fillets in the influence area is evident.<\/jats:p>","DOI":"10.7705\/biomedica.6533","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T21:32:12Z","timestamp":1670880732000},"page":"591-601","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Anisakidae parasites in frozen fish fillets intended for human consumption"],"prefix":"10.7705","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4724-5895","authenticated-orcid":false,"given":"Patricia","family":"Betancourth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9092-544X","authenticated-orcid":false,"given":"Jairo","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7248-5420","authenticated-orcid":false,"given":"Jorge A.","family":"Fern\u00e1ndez-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8211-978X","authenticated-orcid":false,"given":"Juliana","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"112535","doi-asserted-by":"crossref","unstructured":"Furuya K, Nakajima H, Sasaki Y, Urita Y. Anisakiasis: The risks of seafood consumption. Niger J Clin Pract. 2018;21:1492-4. https:\/\/doi.org\/10.4103\/njcp.njcp_256_17","DOI":"10.4103\/njcp.njcp_256_17"},{"key":"112536","doi-asserted-by":"crossref","unstructured":"Pati\u00f1o JA, Olivera MJ. Anisakiasis gastro-al\u00e9rgica, primera descripci\u00f3n de un caso en Colombia y revisi\u00f3n bibliogr\u00e1fica. Biom\u00e9dica. 2019;39:241-6. https:\/\/doi.org\/10.7705\/biomedica.v39i2.3936","DOI":"10.7705\/biomedica.v39i2.3936"},{"key":"112537","doi-asserted-by":"crossref","unstructured":"Castellanos-Garz\u00f3n JA, Falla-Z\u00fa\u00f1iga LF, Salazar L, Pustovrh-Ramos MC. Anis\u00e1kidos y anisakidosis: generalidades y su actualidad en Colombia. Revisi\u00f3n bibliogr\u00e1fica. IATREIA. 2020;33:143-54. https:\/\/doi.org\/10.17533\/udea.iatreia.47","DOI":"10.17533\/udea.iatreia.47"},{"key":"112538","doi-asserted-by":"crossref","unstructured":"de Las Vecillas L, Mu\u00f1oz-Cacho P, L\u00f3pez-Hoyos M, Monttecchiani V, Mart\u00ednez-Sern\u00e1ndez V, Ubeira FM, et al. Analysis of Ani s 7 and Ani s 1 allergens as biomarkers of sensitization and allergy severity in human anisakiasis. Sci Rep. 2020;10:11275. https:\/\/doi.org\/10.1038\/s41598-020-67786-w","DOI":"10.1038\/s41598-020-67786-w"},{"key":"112539","unstructured":"Pardo V, Manuel J. El anisakis y sus enfermedades como enfermedad profesional. Med Segur Trab (Madr). 2016;62:223-40."},{"key":"112540","doi-asserted-by":"crossref","unstructured":"Morsy K, Badr AM, Abdel-Ghaffar F, El Deeb S, Ebead S. Pathogenic potential of fresh, frozen, and thermally treated Anisakis spp. Type II (L3) (Nematoda: Anisakidae) after oral inoculation into Wistar rats: A histopathological study. J Nematol. 2017,49:427-36. https:\/\/doi.org\/10.21307\/jofnem-2017-092","DOI":"10.21307\/jofnem-2017-092"},{"key":"112541","doi-asserted-by":"crossref","unstructured":"Aibinu IE, Smooker PM, Lopata AL. Anisakis nematodes in fish and shellfish- from infection to allergies. Int J Parasitol Parasites Wildl. 2019;9:384-93. https:\/\/doi.org\/10.1016\/j.ijppaw.2019.04.007","DOI":"10.1016\/j.ijppaw.2019.04.007"},{"key":"112542","doi-asserted-by":"crossref","unstructured":"Bao M, Pierce GJ, Pascual S, Gonz\u00e1lez-Mu\u00f1oz M, Mattiucci S, Mladineo I, et al. Assessing the risk of an emerging zoonosis of worldwide concern: Anisakiasis. Sci Rep. 2017;7:43699. https:\/\/doi.org\/10.1038\/srep43699","DOI":"10.1038\/srep43699"},{"key":"112543","doi-asserted-by":"crossref","unstructured":"Amir A, Ngui R, Ismail WHW, Wong KT, Ong JSK, Lim YAL, et al. Anisakiasis causing acute dysentery in Malaysia. Am J Trop Med Hyg. 2016;95:410-2. https:\/\/doi.org\/10.4269\/ajtmh.16-0007","DOI":"10.4269\/ajtmh.16-0007"},{"key":"112544","doi-asserted-by":"crossref","unstructured":"Chung Y-B, Lee J. Clinical characteristics of gastroallergic anisakiasis and diagnostic implications of immunologic tests. Allergy Asthma Immunol Res. 2014;6:228-33. https:\/\/doi.org\/10.4168\/aair.2014.6.3.228","DOI":"10.4168\/aair.2014.6.3.228"},{"key":"112545","doi-asserted-by":"crossref","unstructured":"Restrepo-Betancurt LF, Rodr\u00edguez-Espinosa H, Valencia-Y D. Caracterizaci\u00f3n del consumo de pescado y mariscos en poblaci\u00f3n universitaria de la ciudad de Medell\u00edn - Colombia. Universidad y Salud. 2016;18:257. https:\/\/doi.org\/10.22267\/rus.161802.36","DOI":"10.22267\/rus.161802.36"},{"key":"112546","doi-asserted-by":"crossref","unstructured":"Olivero-Verbel J, Baldiris-\u00c1vila R, G\u00fcette-Fern\u00e1ndez J, Benavides-\u00c1lvarez A, Mercado-Camargo J, Arroyo-Salgado B. Contracaecum sp. infection in Hoplias malabaricus (moncholo) from rivers and marshes of Colombia. Vet Parasitol. 2006;140:90-7. https:\/\/doi.org\/10.1016\/j.vetpar.2006.03.014","DOI":"10.1016\/j.vetpar.2006.03.014"},{"key":"112547","doi-asserted-by":"crossref","unstructured":"Castellanos JA, Santana-Pi\u00f1eros AM, Mercado R, Pe\u00f1a S, Pustovrh C, Cruz-Quintana Y. Presence of anisakid larvae in commercial fishes landed in the Pacific coast of Ecuador and Colombia. Infectio. 2018;22:206. https:\/\/doi.org\/10.22354\/in.v22i4.739","DOI":"10.22354\/in.v22i4.739"},{"key":"112548","unstructured":"CDC-Centers for Disease Control, Prevention. CDC - anisakiasis - frequently asked questions (FAQs). 2010. Accessed: July 16, 2022. Available at: https:\/\/www.cdc.gov\/parasites\/anisakiasis\/faqs.html"},{"key":"112549","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Garc\u00eda JA, Reding-Bernal A, L\u00f3pez-Alvarenga JC. C\u00e1lculo del tama\u00f1o de la muestra en investigaci\u00f3n en educaci\u00f3n m\u00e9dica. Investig Educ M\u00e9dica. 2013;2:217-24.","DOI":"10.1016\/S2007-5057(13)72715-7"},{"key":"112550","unstructured":"Departamento Administrativo Nacional de Estad\u00edstica. Sistema de informaci\u00f3n de precios (SIPSA). Accessed: July 16, 2022. Available at: https:\/\/www.dane.gov.co\/index.php\/estadisticas-por-tema\/agropecuario\/sistema-de-informacion-de-precios-sipsa"},{"key":"112551","doi-asserted-by":"crossref","unstructured":"Karl H, Leineman M. A fast and quantitative detection method for nematodes in fish fillets and fishery products. Archiv f\u00fcr Lebensmittelhygiene. 1993;44:124-5. https:\/\/doi.org\/10.1016\/j.fawpar.2016.07.003","DOI":"10.1016\/j.fawpar.2016.07.003"},{"key":"112552","unstructured":"Keim A. Manual de m\u00e9todos parasitol\u00f3gicos e histopatol\u00f3gicos en piscicultura. 1982. Accessed: July 16, 2022. Available at: https:\/\/aquadocs.org\/handle\/1834\/4913"},{"key":"112553","doi-asserted-by":"crossref","unstructured":"Ishii Y, Fujino T, Weerasooriya MV. Morphology of anisakine larvae. En: Gastric Anisakiasis in Japan. Tokyo: Springer Japan; 1989. p.19-29.","DOI":"10.1007\/978-4-431-68290-5_4"},{"key":"112554","unstructured":"StataCorp. Stata Statistical Software: Release 16. College Station, TX: StataCorp LLC. (2019). Accessed: July 16, 2022. Available at: https:\/\/www.stata.com\/support\/faqs\/resources\/citing-software-documentation-faqs\/"},{"key":"112555","doi-asserted-by":"crossref","unstructured":"Sonko P, Chih-Cheng Chen S, Chou C-M, Huang Y-C, Hsu S-L, Bar\u010d\u00e1k D, et al. Multidisciplinary approach in study of the zoonotic Anisakis larval infection in the blue mackerel (Scomber australasicus) and the largehead hairtail (Trichiurus lepturus) in Northern Taiwan. J Microbiol Immunol Infect. 2020;53:1021-9. https:\/\/doi.org\/10.1016\/j.jmii.2019.04.012","DOI":"10.1016\/j.jmii.2019.04.012"},{"key":"112556","doi-asserted-by":"crossref","unstructured":"Torres-Frenzel P, Torres P. Anisakid parasites in commercial hake ceviche in southern Chile. J Food Prot. 2014;77:1237-40. http:\/\/doi.org\/10.4315\/0362-028X.JFP-13-538","DOI":"10.4315\/0362-028X.JFP-13-538"},{"key":"112557","doi-asserted-by":"crossref","unstructured":"Guardone L, Nucera D, Lodola LB, Tinacci L, Acutis PL, Guidi A, et al. Anisakis spp. larvae in different kinds of ready to eat products made of anchovies (Engraulis encrasicolus) sold in Italian supermarkets. Int J Food Microbiol. 2018;268:10-8. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2017.12.030","DOI":"10.1016\/j.ijfoodmicro.2017.12.030"},{"key":"112558","doi-asserted-by":"crossref","unstructured":"Klapper R, Kuhn T, M\u00fcnster J, Levsen A, Karl H, Klimpel S. Anisakid nematodes in beaked redfish (Sebastes mentella) from three fishing grounds in the North Atlantic, with special notes on distribution in the fish musculature. Vet Parasitol. 2015;207:72-80. https:\/\/doi.org\/10.1016\/j.vetpar.2014.11.017","DOI":"10.1016\/j.vetpar.2014.11.017"},{"key":"112559","doi-asserted-by":"crossref","unstructured":"Guardone L, Nucera D, Pergola V, Costanzo F, Costa E, Tinacci L, et al. Visceral larvae as a predictive index of the overall level of fish batch infection in European anchovies (Engraulis encrasicolus): A rapid procedure for food business operators to assess marketability. Int J Food Microbiol. 2017;250:12-8. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2017.03.011","DOI":"10.1016\/j.ijfoodmicro.2017.03.011"},{"key":"112560","doi-asserted-by":"crossref","unstructured":"Mercken E, van Damme I, Vangeenberghe S, Serradell A, De Sterck T, Lumain JPL, et al. Ascaridoids in commercial fish: Occurrence, intensity and localization in whole fish and fillets destined for the Belgian market. Int J Food Microbiol. 2020;327:108657. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2020.108657","DOI":"10.1016\/j.ijfoodmicro.2020.108657"},{"key":"112561","doi-asserted-by":"crossref","unstructured":"Suzuki J, Murata R, Hosaka M, Araki J. Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes. Int J Food Microbiol. 2010;137:88-93. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2009.10.001","DOI":"10.1016\/j.ijfoodmicro.2009.10.001"},{"key":"112562","doi-asserted-by":"crossref","unstructured":"Colombo F, Cattaneo P, Castelletti M, Bernardi C. Prevalence and mean intensity of Anisakidae parasite in seafood caught in the Mediterranean Sea focusing on fish species at risk of being raw-consumed. A metaanalysis and systematic review. Crit Rev Food Sci Nutr. 2016;56:1405-16. https:\/\/doi.org\/10.1080\/10408398.2012.755947","DOI":"10.1080\/10408398.2012.755947"},{"key":"112563","doi-asserted-by":"crossref","unstructured":"Wang X, Jia H, Gong H, Zhang Y, Mi R, Zhang Y, et al. Expression and functionality of allergenic genes regulated by simulated gastric juice in Anisakis pegreffii. Parasitol Int. 2021;80:102223. https:\/\/doi.org\/10.1016\/j.parint.2020.102223","DOI":"10.1016\/j.parint.2020.102223"},{"key":"112564","doi-asserted-by":"crossref","unstructured":"G\u00f3mez-Morales MA, Castro CM, Lalle M, Fern\u00e1ndez R, Pezzotti P, Abollo E, et al. UV-press method versus artificial digestion method to detect Anisakidae L3 in fish fillets: Comparative study and suitability for the industry. Fish Res. 2018;202:22-8. https:\/\/doi.org\/10.1016\/j.fishres.2016.12.011","DOI":"10.1016\/j.fishres.2016.12.011"},{"key":"112565","doi-asserted-by":"crossref","unstructured":"Levsen A, Maage A. Absence of parasitic nematodes in farmed, harvest quality Atlantic salmon (Salmo salar) in Norway \u2013 Results from a large scale survey. Food Control. 2016;68:25-9. https:\/\/doi.org\/10.1016\/j.foodcont.2016.03.020","DOI":"10.1016\/j.foodcont.2016.03.020"},{"key":"112566","doi-asserted-by":"crossref","unstructured":"Cipriani P, Giulietti L, Palomba M, Mattiucci S, Bao M, Levsen A. Occurrence of larval ascaridoid nematodes in the Argentinean short-finned squid Illex argentinus from the Southwest Atlantic Ocean (off Falkland Islands). Int J Food Microbiol. 2019;297:27-31. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2019.02.019","DOI":"10.1016\/j.ijfoodmicro.2019.02.019"},{"key":"112567","doi-asserted-by":"crossref","unstructured":"Villafruela-Cives M, Henr\u00edquez-Santana A. Anisakiasis. Rev Esp Enferm Dig. 2010;102:216.","DOI":"10.4321\/S1130-01082010000300010"},{"key":"112568","doi-asserted-by":"crossref","unstructured":"Mattiucci S, Cipriani P, Levsen A, Paoletti M, Nascetti G. Molecular epidemiology of Anisakis and anisakiasis: An ecological and evolutionary road map. Adv Parasitol. 2018;99:93-263. https:\/\/doi.org\/10.1016\/bs.apar.2017.12.001","DOI":"10.1016\/bs.apar.2017.12.001"},{"key":"112569","doi-asserted-by":"crossref","unstructured":"Moreno LT. La pesca de los pescadores artesanales en Colombia. PEGADA - A Revista da Geografia do Trabalho. 2018;19. https:\/\/doi.org\/10.33026\/peg.v19i2.5514","DOI":"10.33026\/peg.v19i2.5514"},{"key":"112570","doi-asserted-by":"crossref","unstructured":"Lucano-Ram\u00edrez G, Ruiz-Ram\u00edrez S, Palomera-S\u00e1nchez FI, Gonz\u00e1lez-Sans\u00f3n G. Biolog\u00eda reproductiva de la sierra Scomberomorus sierra (Pisces, Scombridae) en el Pac\u00edfico central mexicano. Cienc Mar. 2011;37:249-60.","DOI":"10.7773\/cm.v37i3.1892"},{"key":"112571","doi-asserted-by":"crossref","unstructured":"Abattouy N, L\u00f3pez AV, Maldonado JL, Benajiba MH, Mart\u00edn-S\u00e1nchez J. Epidemiology and molecular identification of Anisakis pegreffii (Nematoda: Anisakidae) in the horse mackerel Trachurus trachurus from northern Morocco. J Helminthol. 2014;88:257-63. https:\/\/doi.org\/10.1017\/S0022149X13000102","DOI":"10.1017\/S0022149X13000102"},{"key":"112572","unstructured":"Lerena MSM, Torres EP, Ram\u00edrez M\u00c1P, Orozco JAH, Estrada GE, Esparza CD. Presencia de par\u00e1sitos en pescado en el mercado local de la Zona Metropolitana de Guadalajara. e-CUCBA. 2018:21-6."}],"container-title":["Biom\u00e9dica"],"original-title":[],"link":[{"URL":"https:\/\/revistabiomedica.org\/index.php\/biomedica\/article\/download\/6533\/5103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/revistabiomedica.org\/index.php\/biomedica\/article\/download\/6533\/5103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T21:32:20Z","timestamp":1701552740000},"score":1,"resource":{"primary":{"URL":"https:\/\/revistabiomedica.org\/index.php\/biomedica\/article\/view\/6533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,1]]},"references-count":38,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,12,1]]}},"URL":"https:\/\/doi.org\/10.7705\/biomedica.6533","relation":{},"ISSN":["2590-7379","0120-4157"],"issn-type":[{"type":"electronic","value":"2590-7379"},{"type":"print","value":"0120-4157"}],"subject":[],"published":{"date-parts":[[2022,12,1]]},"assertion":[{"value":"2022-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer Reviewed","group":{"name":"peer_review","label":"Peer Review"}},{"value":"Instituto Nacional de Salud","name":"publisher","label":"This article is maintained by"},{"value":"Biomedica","name":"journaltitle","label":"Journal Title"},{"value":"Art\u00edculos originales","name":"content_type","label":"Content Type"}]}}