{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T02:40:05Z","timestamp":1773369605880,"version":"3.50.1"},"reference-count":23,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"3","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. La resistencia de Plasmodium falciparum a diferentes f\u00e1rmacos antipal\u00fadicos es un obst\u00e1culo para eliminar la enfermedad. El genotipo resistente de P. falciparum a la artemisinina puede evaluarse examinando los polimorfismos en el dominio de la h\u00e9lice del gen Pfk13. La Organizaci\u00f3n Mundial de la Salud recomienda utilizar estas mutaciones como marcadores moleculares para detectar la resistencia a la artemisinina en pa\u00edses donde la malaria por P. falciparum es end\u00e9mica.Objetivo. Identificar mutaciones relacionadas con la resistencia a artemisinina presentes en el dominio de la h\u00e9lice del gen k13 de P. falciparum.Materiales y m\u00e9todos. Mediante la detecci\u00f3n pasiva de casos, se recolectaron 51 muestras positivas por microscop\u00eda para Plasmodium, provenientes de seis comunidades del distrito de R\u00edo Santiago en Condorcanqui, Amazonas. Se realiz\u00f3 la confirmaci\u00f3n molecular de la especie mediante PCR en tiempo real y el dominio de la h\u00e9lice del gen Pfk13 se amplific\u00f3 y secuenci\u00f3 por electroforesis capilar. Las secuencias obtenidas se compararon con la cepa de referencia 3D7 de fenotipo silvestre.Resultados. Se confirm\u00f3 un total de 51 muestras positivas para P. falciparum, provenientes de las comunidades de Ayambis, Chapiza, Palometa, Muchinguis, Alianza Progreso y Caterpiza. Despu\u00e9s del alineamiento de las secuencias de ADN, se determin\u00f3 que las muestras no presentaron mutaciones asociadas con resistencia en el gen K13. Discusi\u00f3n. Los resultados obtenidos son coherentes con estudios similares realizados en otros pa\u00edses de Sudam\u00e9rica, incluyendo Per\u00fa. Estos datos proporcionan una l\u00ednea base para la vigilancia molecular de resistencia a artemisinina en la regi\u00f3n Amazonas y refuerzan la eficacia de la terapia combinada con artemisinina en esta \u00e1rea.<\/jats:p>","DOI":"10.7705\/biomedica.6849","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T16:31:03Z","timestamp":1697819463000},"page":"352-359","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Caracterizaci\u00f3n molecular del dominio de la h\u00e9lice del gen k13 de Plasmodium falciparum en muestras de comunidades nativas de Condorcanqui, Amazonas, Per\u00fa","Molecular characterization of the Plasmodium falciparum k13 gene helix domain in samples from native communities of Condorcanqui, Amazonas, Per\u00fa"],"prefix":"10.7705","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2780-6103","authenticated-orcid":false,"given":"Julio","family":"Sandoval-Bances","sequence":"first","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2217-9561","authenticated-orcid":false,"given":"Milagros","family":"Saavedra-Samill\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4823-2778","authenticated-orcid":false,"given":"Sonia","family":"Huyhua-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa;\u00a0Facultad de Ciencias de la Salud, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa;\u00a0Facultad de Ciencias de la Salud, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8453-5501","authenticated-orcid":false,"given":"Luis M.","family":"Rojas","sequence":"additional","affiliation":[{"name":"Direcci\u00f3n Regional de Salud de Amazonas, Chachapoyas, Per\u00fa"},{"name":"Direcci\u00f3n Regional de Salud de Amazonas, Chachapoyas, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1181-8540","authenticated-orcid":false,"given":"Sonia","family":"Tejada-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Facultad de Ciencias de la Salud, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Facultad de Ciencias de la Salud, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7483-1729","authenticated-orcid":false,"given":"Rafael","family":"Tapia-Limonchi","sequence":"additional","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Instituto de Investigaciones en Ciencias Biom\u00e9dicas, Universidad Ricardo Palma, Lima, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Instituto de Investigaciones en Ciencias Biom\u00e9dicas, Universidad Ricardo Palma, Lima, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-0664","authenticated-orcid":false,"given":"Stella M.","family":"Chenet","sequence":"additional","affiliation":[{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Instituto de Investigaciones en Ciencias Biom\u00e9dicas, Universidad Ricardo Palma, Lima, Per\u00fa"},{"name":"Instituto de Enfermedades Tropicales, Universidad Nacional Toribio Rodr\u00edguez de Mendoza de Amazonas, Triunfo, Chachapoyas, Per\u00fa; Instituto de Investigaciones en Ciencias Biom\u00e9dicas, Universidad Ricardo Palma, Lima, Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"125716","doi-asserted-by":"crossref","unstructured":"Chapadense F, Machado RL, Ventura AMR da S, \u00c1reas A, Machado RB, Viana GR, et al. 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