{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T01:12:37Z","timestamp":1773364357639,"version":"3.50.1"},"reference-count":31,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"1","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. El s\u00edndrome de Sj\u00f6gren es una enfermedad autoinmunitaria sist\u00e9mica. Se ha descrito la utilidad de la inmunohistoqu\u00edmica de la biopsia de gl\u00e1ndula salival menor por su utilidad en la caracterizaci\u00f3n del fenotipo de los linfocitos en los casos de dif\u00edcil diagn\u00f3stico.Objetivo. Describir las variables sociodemogr\u00e1ficas, cl\u00ednicas, serol\u00f3gicas, histopatol\u00f3gicas e inmunohistoqu\u00edmicas de los pacientes con s\u00edndrome seco en quienes se practic\u00f3 biopsia de gl\u00e1ndula salival menor que result\u00f3 con un puntaje de foco de uno o m\u00e1s.Materiales y m\u00e9todos. Se adelant\u00f3 un estudio observacional, retrospectivo, en el que se incluyeron pacientes en estudio por posible s\u00edndrome seco y cuya biopsia de gl\u00e1ndula salival menor estaba disponible y hab\u00eda obtenido un puntaje de foco de uno o m\u00e1s. En la biopsia de la gl\u00e1ndula salival menor se realiz\u00f3 inmunohistoqu\u00edmica con crom\u00f3geno rojo para la identificaci\u00f3n de los linfocitos T CD8 positivos y marr\u00f3n, para los linfocitos T CD4 positivos. Se determin\u00f3 la relaci\u00f3n de los marcadores CD20:CD3 y CD4:CD8 con el equipo MoticEasyScan Pro-6\u2122 (MOTIC) y el software QuPath\u2122. El an\u00e1lisis de las variables cualitativas se realiz\u00f3 mediante la aplicaci\u00f3n de la prueba de ji al cuadrado (c2) o la prueba exacta de Fisher; para el de las variables cuantitativas, la prueba seleccion\u00f3 seg\u00fan el supuesto de normalidad.Resultados. Se analizaron 28 pacientes, 16 con s\u00edndrome de Sj\u00f6gren y 8 con poliautoinmunidad. Se encontr\u00f3 una asociaci\u00f3n entre la presencia de atrofia glandular en la biopsia de gl\u00e1ndula salival menor y el desarrollo de poliautoinmunidad (OR = 11,1; IC 95%: 1,12-112; p = 0,033). Las relaciones CD20:CD3 y CD4:CD8 fueron normales, sin diferencias estad\u00edsticamente significativas entre pacientes con s\u00edndrome de Sj\u00f6gren y sin \u00e9l. En el subgrupo de pacientes con s\u00edndrome de Sj\u00f6gren hubo predominancia de los linfocitos T CD4, 15 de los 16 casos ten\u00edan una relaci\u00f3n CD4:CD8 igual o mayor de 2:1. Conclusiones. Se encontr\u00f3 una asociaci\u00f3n entre la atrofia glandular y la presencia de poliautoinmunidad, as\u00ed como predominancia de los linfocitos T CD4 en los pacientes con s\u00edndrome de Sj\u00f6gren. Esto resalta el posible valor de aplicar la inmunohistoqu\u00edmica en las biopsias de gl\u00e1ndula salival menor en este grupo.<\/jats:p>","DOI":"10.7705\/biomedica.7315","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T15:23:56Z","timestamp":1743434636000},"page":"80-93","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Caracter\u00edsticas cl\u00ednicas, histopatol\u00f3gicas e inmunohistoqu\u00edmicas de pacientes con s\u00edndrome seco y biopsia de gl\u00e1ndula salival con puntaje de foco de 1 o m\u00e1s","Clinical, histopathological, and immunohistochemical characteristics of patients with sicca syndrome with a focus score \u2265 1 in the minor salivary gland biopsy"],"prefix":"10.7705","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1783-6598","authenticated-orcid":false,"given":"Andr\u00e9s Felipe","family":"Lamos-Duarte","sequence":"first","affiliation":[{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. C., Colombia"},{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8428-5837","authenticated-orcid":false,"given":"Rafael","family":"Parra-Medina","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda, Instituto Nacional de Cancerolog\u00eda y Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Bogot\u00e1, D. C., Colombia"},{"name":"Laboratorio de Patolog\u00eda, Instituto Nacional de Cancerolog\u00eda y Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2674-285X","authenticated-orcid":false,"given":"Carlos Santiago","family":"Rivadeneira-Chamorro","sequence":"additional","affiliation":[{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. C., Colombia"},{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5400-9068","authenticated-orcid":false,"given":"Juan Pablo","family":"Casta\u00f1eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Bogot\u00e1, D. C., Colombia"},{"name":"Instituto de Investigaciones, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7125-1462","authenticated-orcid":false,"given":"Alejandro","family":"Escobar","sequence":"additional","affiliation":[{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. C., Colombia"},{"name":"Departamento de Reumatolog\u00eda, Fundaci\u00f3n Universitaria de Ciencias de la Salud - FUCS, Hospital de San Jos\u00e9, Bogot\u00e1, D. 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Experiencia en el uso de los protocolos BIOMED-2 para el estudio de reordenamientos de TCR e inmunoglobulinas en proliferaciones linfoides en el Instituto Nacional de Cancerolog\u00eda, Colombia. Biom\u00e9dica. 2022;42:64. https:\/\/doi.org\/10.7705\/biomedica.5940","DOI":"10.7705\/biomedica.5940"},{"key":"144927","doi-asserted-by":"crossref","unstructured":"11. Zhang Y, Yu D, Huang K, Huang C, Liu H, Sun X, et al. Evaluation of the diagnostic value of immunoglobulin clonal gene rearrangements in patients with parotid gland MALT lymphoma using BIOMED-2 protocol. Oral Surg Oral Med Oral Pathol Oral Radiol 2018;126:165-73. https:\/\/doi.org\/10.1016\/J.OOOO.2018.03.005","DOI":"10.1016\/j.oooo.2018.03.005"},{"key":"144928","doi-asserted-by":"crossref","unstructured":"12. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. 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Study of clinical factors, focus score, lymphocyte type and NF-\u03baB pathway in Sj\u00f6gren\u2019s syndrome. Odontology. 2023;111:207-16. https:\/\/doi.org\/10.1007\/S10266-022-00728-2","DOI":"10.1007\/s10266-022-00728-2"},{"key":"144946","doi-asserted-by":"crossref","unstructured":"30. van Ginkel MS, van der Sluis T, Bulthuis MLC, Buikema HJ, Haacke EA, Arends S, et al. Digital image analysis of intraepithelial B-lymphocytes to assess lymphoepithelial lesions in salivary glands of Sj\u00f6gren\u2019s syndrome patients. Rheumatology (Oxford). 2022;62:428-38. https:\/\/doi.org\/10.1093\/rheumatology\/keac212","DOI":"10.1093\/rheumatology\/keac212"},{"key":"144947","doi-asserted-by":"crossref","unstructured":"31. Trivedi A, Cornejo KM, O\u2019Donnell P, Dresser K, Deng A. Employing immunohistochemical staining to labial minor salivary gland biopsies from patients with Sjogren\u2019s syndrome increases diagnostic certainty. 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