{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:09:13Z","timestamp":1787836153857,"version":"build-2784847793"},"reference-count":23,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"Sp. 2","license":[{"start":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T00:00:00Z","timestamp":1734912000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T00:00:00Z","timestamp":1734912000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. La enfermedad granulomatosa cr\u00f3nica es un defecto de la fagocitosis por deficiencia de gp91phox, p22phox, p47phox, p40phox y p67phox (forma cl\u00e1sica). Recientemente, se describi\u00f3 la deficiencia de EROS y p40phox como responsables de la forma no cl\u00e1sica. La t\u00e9cnica de oxidaci\u00f3n de la 1,2,3-dihidrorrodamina \u2013usando como est\u00edmulo el forbol-12-miristato-13-acetato\u2013 se utiliza para el diagn\u00f3stico de la enfermedad granulomatosa cr\u00f3nica cl\u00e1sica. Sin embargo, para la detecci\u00f3n de la oxidaci\u00f3n mediada por EROS y p40phox, se requiere de otros est\u00edmulos como zimos\u00e1n, Escherichia coli o Staphylococcus aureus.Objetivo. Estandarizar el est\u00edmulo con zimos\u00e1n en la t\u00e9cnica de la 1,2,3-dihidrorrodamina para evaluar el estallido respiratorio del neutr\u00f3filo.Materiales y m\u00e9todos. Se obtuvo sangre de cinco sujetos sanos previo consentimiento informado. Se utiliz\u00f3 la t\u00e9cnica de 1,2,3-dihidrorrodamina usando el forbol-12-miristato-13-acetato como control y diferentes cantidades de zimos\u00e1n opsonizado (150, 100, 50, 20 y 10 \u03bcg). Por citometr\u00eda de flujo, se obtuvo la intensidad de fluorescencia media de la rodamina 1,2,3 en las poblaciones de neutr\u00f3filos y se calcul\u00f3 el \u00edndice de oxidaci\u00f3n. Se utilizaron las pruebas de Kolmogorov-Smirnov, ANOVA y el an\u00e1lisis post-hoc de Tukey. Se consider\u00f3 un valor de p \u2264 0,05 como estad\u00edsticamente significativo.Resultados. El forbol-12-miristato-13-acetato increment\u00f3 la intensidad de la fluorescencia media de la rodamina 1,2,3 en los sujetos sanos. Entre las diferentes condiciones de zimos\u00e1n probadas, la de 50 \u03bcg fue la cantidad \u00f3ptima y reproducible en todos los controles por el an\u00e1lisis estad\u00edstico y los hallazgos citom\u00e9tricos.Conclusiones. Se presenta la optimizaci\u00f3n de la t\u00e9cnica 1,2,3-dihidrorrodamina empleando el zimos\u00e1n como est\u00edmulo.<\/jats:p>","DOI":"10.7705\/biomedica.7461","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T15:50:52Z","timestamp":1736437852000},"page":"198-208","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Estandarizaci\u00f3n de zimos\u00e1n opsonizado como est\u00edmulo en la t\u00e9cnica de la 1,2,3-dihidrorrodamina para evaluar el estallido respiratorio del neutr\u00f3filo","Standardization of the use of opsonized zymosan as stimulus in the 1,2,3-dihydrorhodamine technique for the assessment of neutrophil respiratory burst"],"prefix":"10.7705","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2156-2166","authenticated-orcid":false,"given":"Uriel","family":"P\u00e9rez-Blanco","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, M\u00e9xico"},{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7027-9615","authenticated-orcid":false,"given":"Jenniffer Yissel","family":"Gir\u00f3n","sequence":"additional","affiliation":[{"name":"Red de Apoyo a la Investigaci\u00f3n, Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"},{"name":"Red de Apoyo a la Investigaci\u00f3n, Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0633-0567","authenticated-orcid":false,"given":"Guillermo","family":"Ju\u00e1rez -Vega","sequence":"additional","affiliation":[{"name":"Red de Apoyo a la Investigaci\u00f3n, Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"},{"name":"Red de Apoyo a la Investigaci\u00f3n, Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1437-5214","authenticated-orcid":false,"given":"Mar\u00eda","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, M\u00e9xico"},{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6263-442X","authenticated-orcid":false,"given":"Carlos","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, M\u00e9xico"},{"name":"Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatr\u00eda, Ciudad de M\u00e9xico, 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