{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:16:11Z","timestamp":1783052171721,"version":"3.54.6"},"reference-count":82,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"2","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. El municipio de Ovejas en Los Montes de Mar\u00eda es un foco urbano aut\u00f3ctono de leishmaniasis y registra casos espor\u00e1dicos de la enfermedad de Chagas. Aunque Didelphis marsupialis (zarig\u00fceya) es reconocido como hu\u00e9sped, hospedador u hospedero, rural de tripanosomat\u00eddeos en Colombia, se desconoce su papel en esta zona del pa\u00eds.Objetivo. Evaluar la frecuencia de infecci\u00f3n natural por Leishmania spp. y Trypanosoma cruzi en D. marsupialis de ambientes peridomiciliarios del municipio de Ovejas.Materiales y m\u00e9todos. Entre agosto y octubre del 2015 se capturaron zarig\u00fceyas en las \u00e1reas urbanas con trampas de vida Tomahawk. Tras evaluaci\u00f3n morfom\u00e9trica, identificaci\u00f3n del sexo y conteo de piezas dentales, se obtuvieron muestras sangu\u00edneas por punci\u00f3n cardiaca, usadas para detectar anticuerpos IgG contra Leishmania spp. y T. cruzi, amplificar blancos gen\u00e9ticos espec\u00edficos de estos par\u00e1sitos y para aislamiento in vitro. Los par\u00e1sitos se tipificaron mediante an\u00e1lisis filogen\u00e9tico de los genes citocromo b y hsp70.Resultados. Se capturaron 26 individuos (12 machos y 14 hembras; 16 adultos, 7 subadultos y 3 juveniles). Se obtuvo una prevalencia de anticuerpos del 23,08 y 53,85 %, contra Leishmania spp. y T. cruzi, respectivamente, con 11,54 % de coinfecci\u00f3n. La frecuencia de infecci\u00f3n molecular por T. cruzi fue del 61,54 y 23,08 % para Leishmania spp. Se logr\u00f3 aislar y genotipificar una cepa de T. cruzi.Conclusi\u00f3n. Las altas frecuencias de infecci\u00f3n simple y coinfecci\u00f3n indican que D. marsupialis podr\u00eda actuar como hu\u00e9sped sinantr\u00f3pico de Leishmania spp. y T. cruzi en Ovejas, sugiriendo su participaci\u00f3n en el mantenimiento de los ciclos dom\u00e9sticos de transmisi\u00f3n urbana.<\/jats:p>","DOI":"10.7705\/biomedica.7827","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:11:52Z","timestamp":1782321112000},"page":"270-289","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Coinfecci\u00f3n por Leishmania spp. y Trypanosoma cruzi en Didelphis marsupialis (Mammalia: Didelphimorphia) del \u00e1rea urbana de Ovejas, Colombia","Co-infection by Leishmania spp. and Trypanosoma cruzi in Didelphis marsupialis (Mammalia: Didelphimorphia) from urban areas of Ovejas, Colombia"],"prefix":"10.7705","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5942-7644","authenticated-orcid":false,"given":"Jorge Luis","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1473-5895","authenticated-orcid":false,"given":"Alveiro","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia; Divisi\u00f3n de Investigaci\u00f3n, Innovaci\u00f3n y Desarrollo, Pyrogen S.A.S., Sincelejo, Colombia"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia; Divisi\u00f3n de Investigaci\u00f3n, Innovaci\u00f3n y Desarrollo, Pyrogen S.A.S., Sincelejo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-645X","authenticated-orcid":false,"given":"Matilde","family":"Rivero","sequence":"additional","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9253-703X","authenticated-orcid":false,"given":"Carolina","family":"Ayala","sequence":"additional","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9619-7711","authenticated-orcid":false,"given":"Luis Gregorio","family":"Estrada","sequence":"additional","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia; Instituto Nacional de Medicina Tropical, ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d, Ministerio de Salud de la Naci\u00f3n, Puerto Iguaz\u00fa, Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires, Argentina"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia; Instituto Nacional de Medicina Tropical, ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d, Ministerio de Salud de la Naci\u00f3n, Puerto Iguaz\u00fa, Argentina; Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3475-0075","authenticated-orcid":false,"given":"Samanta Cristina","family":"Das Chagas","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de Tripanosom\u00e1tidos, Instituto Oswaldo Cruz, R\u00edo de Janeiro, Brasil"},{"name":"Laboratorio de Biolog\u00eda de Tripanosom\u00e1tidos, Instituto Oswaldo Cruz, R\u00edo de Janeiro, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3076-455X","authenticated-orcid":false,"given":"Eduar El\u00edas","family":"Bejarano","sequence":"additional","affiliation":[{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"},{"name":"Grupo Investigaciones Biom\u00e9dicas, Universidad de Sucre, Sincelejo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4444","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"164763","unstructured":"Mott KE, Desjeux P, Moncayo A, Ranque P, de Raadt P. 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