{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:06:46Z","timestamp":1790021206287,"version":"4.0.1"},"reference-count":27,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"3","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. El sistema nervioso es uno de los blancos del virus del Zika. Es fundamental disponer de reactivos biol\u00f3gicos para apoyo diagn\u00f3stico e investigaciones sobre patog\u00e9nesis que requieren tejidos fijados en aldeh\u00eddos.Objetivo. Determinar la utilidad de dos antisueros elaborados para la detecci\u00f3n de ant\u00edgenos del virus del Zika en c\u00e9lulas y tejidos fijados en aldeh\u00eddos.Materiales y m\u00e9todos. Los antisueros se produjeron en conejos Nueva Zelanda a partir de un aislamiento viral del brote de Zika del 2016 en Colombia. En uno de los animales se emple\u00f3 adyuvante de Freund para los refuerzos y, en el otro, el adyuvante Montanide. Se realizaron titulaciones por inmunorreacciones en c\u00e9lulas y tejidos positivos para el virus del Zika. Se emplearon los m\u00e9todos de estreptavidina-fosfatasa alcalina y avidina-biotinaperoxidasa.Los antisueros se evaluaron en muestras infectadas experimentalmente para el virus del Zika y para otros virus relacionados y no relacionados; tambi\u00e9n, se evaluaron con muestras de otras t\u00e9cnicas utilizadas en el laboratorio, como cortes obtenidos con vibr\u00e1tomo, muestras para microscop\u00eda \u00f3ptica de alta resoluci\u00f3n y microscop\u00eda electr\u00f3nica de transmisi\u00f3n.Resultados. Hubo inmunorreactividad a los ant\u00edgenos del virus del Zika con ambos antisueros, principalmente en las neuronas de la corteza cerebral. Se demostr\u00f3 inmunotinci\u00f3n en las muestras de cultivos celulares y en los tejidos de animales infectados experimentalmente. Hubo inmunorreactividad cruzada con los virus del dengue y de la fiebre amarilla, pero no con el virus de la rabia.<\/jats:p>","DOI":"10.7705\/biomedica.7857","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:09:31Z","timestamp":1788268171000},"page":"489-498","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Elaboraci\u00f3n y evaluaci\u00f3n de un antisuero para la detecci\u00f3n inmunohistoqu\u00edmica del virus del Zika en tejidos fijados en aldeh\u00eddos","Preparation and evaluation of antisera for immunohistochemical detection of Zika virus in aldehyde-fixed tissues"],"prefix":"10.7705","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7693-6206","authenticated-orcid":false,"given":"Ladys","family":"Sarmiento-Lacera","sequence":"first","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud, Bogot\u00e1, D. C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2865-5266","authenticated-orcid":false,"given":"Gerardo","family":"Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud, Bogot\u00e1, D. C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1927-6798","authenticated-orcid":false,"given":"Claudia Yadira","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud, Bogot\u00e1, D. C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1648-8912","authenticated-orcid":false,"given":"Alicia","family":"Rosales","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud, Bogot\u00e1, D. 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C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0903-2001","authenticated-orcid":false,"given":"Jorge","family":"Rivera","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud, Bogot\u00e1, D. C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5112-5960","authenticated-orcid":false,"given":"Alejandra","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Grupo Animales de Laboratorio, Instituto Nacional de Salud, Bogot\u00e1, D. C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4419-9647","authenticated-orcid":false,"given":"Luz Mila","family":"Pinz\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo Aseguramiento de la Calidad, Instituto Nacional de Salud, Bogot\u00e1, D. 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