{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T19:39:50Z","timestamp":1783021190039,"version":"3.54.6"},"reference-count":60,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"Sp. 1","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100020907","name":"Instituto Nacional de Salud","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020907","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introducci\u00f3n. La listeriosis es una enfermedad transmitida por alimentos asociada con la infecci\u00f3n por Listeria monocytogenes, con gran mortalidad en individuos con el sistema inmunol\u00f3gico debilitado. En Colombia, no es obligatorio notificar la enfermedad.Objetivo. Caracterizar fenot\u00edpica y gen\u00f3micamente los aislamientos cl\u00ednicos de L. monocytogenes realizados entre el 2010 y el 2017 en Colombia.Materiales y m\u00e9todos. Se analizaron 63 de 443 aislamientos de L. monocytogenes de la Vigilancia por Laboratorio del Grupo de Microbiolog\u00eda del Instituto Nacional de Salud. Se practicaron pruebas de sensibilidad antimicrobiana, serolog\u00eda para ant\u00edgenos (O-H) y PCR, PFGE-ApaI, as\u00ed como secuenciaci\u00f3n del genoma completo. Los casos fatales atribuidos a L. monocytogenes fueron identificados en el Sistema Nacional de Vigilancia en Salud P\u00fablica (SIVIGILA).Resultados. De los aislamientos, la mayor\u00eda correspondi\u00f3 al sexo femenino (n = 32; 50,7%). En cuanto a la distribuci\u00f3n por edad, la mayor\u00eda de los casos se present\u00f3 en el grupo de 15 a 54 a\u00f1os (n = 21; 33,3%). La mayor\u00eda de los aislamientos provino de hemocultivos (n = 37; 58,7%), con sensibilidad a la penicilina y al trimetoprim-sulfametoxazol. El 77,8% de los aislamientos correspondi\u00f3 al complejo serogrupo 4b-4d-4e; se determinaron 42 patrones PFGE-ApaI. La secuenciaci\u00f3n gen\u00f3mica de cinco aislamientos permiti\u00f3 identificar, mediante tipificaci\u00f3n por secuenciaci\u00f3n multilocus (MLST), los tipos ST1 y ST2; mediante MLST ribos\u00f3mico (rMLST), los tipos 15992, 15983, 15959 y 98481; los complejos clonales CC1 y CC2; el linaje I y el serotipo 4b in silico. Los genes de virulencia identificados incluyeron islas de pathogencidad LIPI-1, LIPI-3 e internalinas; los genes de resistencia fueron fosX, fosS-2, lin, norB y mprF. Se identificaron el pl\u00e1smido J1776 y los profagos vB_LmoS_293, LP_030_2, LP_030_3 y A118. El an\u00e1lisis filogen\u00e9tico global mostr\u00f3 que las secuencias m\u00e1s cercanas pertenecen al linaje I y provienen de Francia, Chile, Per\u00fa e India.Conclusi\u00f3n. El fortalecimiento en el seguimiento epidemiol\u00f3gico y la tipificaci\u00f3n permiten mejorar las medidas de control en salud p\u00fablica.<\/jats:p>","DOI":"10.7705\/biomedica.7863","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:54:25Z","timestamp":1783004065000},"page":"99-117","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Diversidad gen\u00e9tica de aislamientos de Listeria monocytogenes en Colombia de 2010-2017 y comparaci\u00f3n con la red de vigilancia regional de PulseNet","Genetic diversity of Listeria monocytogenes isolates in Colombia from 2010 until 2017 and comparison against the PulseNet regional surveillance network"],"prefix":"10.7705","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8252-5622","authenticated-orcid":false,"given":"Paula Luc\u00eda","family":"D\u00edaz","sequence":"first","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7290-6383","authenticated-orcid":false,"given":"Julieth Carolina","family":"Gamba","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6326-2003","authenticated-orcid":false,"given":"Diego","family":"Prada","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0083-211X","authenticated-orcid":false,"given":"Lucy Angeline","family":"Monta\u00f1o","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5537-1923","authenticated-orcid":false,"given":"Edna Catering","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-6281","authenticated-orcid":false,"given":"Efra\u00edn","family":"Montilla-Escudero","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6941-635X","authenticated-orcid":false,"given":"Paloma","family":"Cuenca","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8629-0840","authenticated-orcid":false,"given":"Sandra","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1201-7681","authenticated-orcid":false,"given":"Nathaly","family":"Rozo","sequence":"additional","affiliation":[{"name":"Grupo de Mortalidad Perinatal y Neonatal Tard\u00eda, Equipo de Maternidad Segura, Subdirecci\u00f3n de Prevenci\u00f3n y Vigilancia y Control en Salud P\u00fablica, Instituto Nacional de Salud, Bogot\u00e1, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3074-2035","authenticated-orcid":false,"given":"M\u00f3nica","family":"Prieto","sequence":"additional","affiliation":[{"name":"PulseNet Latin America and Caribbean Network, Subregional, Instituto \u201cCarlos Malbr\u00e1n\u201d, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9510-9564","authenticated-orcid":false,"given":"Claudia","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"PulseNet Latin America and Caribbean Network, Subregional, Instituto \u201cCarlos Malbr\u00e1n\u201d, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-3949","authenticated-orcid":false,"given":"Isabel","family":"Chinen","sequence":"additional","affiliation":[{"name":"PulseNet Latin America and Caribbean Network, Subregional, Instituto \u201cCarlos Malbr\u00e1n\u201d, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4444","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"162085","doi-asserted-by":"crossref","unstructured":"Swaminathan B, Gerner-Smidt P. 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