{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:47:41Z","timestamp":1773323261034,"version":"3.50.1"},"reference-count":30,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"Sp. 2","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007886","name":"Fondation Nestl\u00e9","doi-asserted-by":"publisher","award":["Reference 45\/2016"],"award-info":[{"award-number":["Reference 45\/2016"]}],"id":[{"id":"10.13039\/501100007886","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007886","name":"Fondation Nestl\u00e9","doi-asserted-by":"publisher","award":["Reference 45\/2016"],"award-info":[{"award-number":["Reference 45\/2016"]}],"id":[{"id":"10.13039\/501100007886","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["Biomed."],"abstract":"<jats:p>Introduction. Enteric viruses significantly impact morbidity, mortality, and healthcare. Transmission through wastewater is favoured in highly contaminated areas due to inadequate treatment.Objective. To determine the number of rotaviruses and their infectious capacity from wastewater samples used for irrigation in the western part of Bogot\u00e1. Materials and methods. Concentrations of group A rotavirus were monitored in wastewater using molecular methods. The infectivity of rotaviruses was evaluated in a mouse intestinal villi model. We assessed the feasibility of applying this approach for environmental health surveillance in Colombia, considering findings reported by other authors.Results. The research focused on the La Ramada irrigation network in the western part of Bogot\u00e1, specifically the Canal San Jos\u00e9. We analysed eighteen wastewater samples using qRT-PCR and detected group A rotavirus in twelve of them. The positive samples contained infectious rotavirus, as confirmed through the mouse villi model.Conclusion. This study shows that contamination by group A rotavirus is frequent in wastewaters from the Canal San Jos\u00e9 in the La Ramada irrigation network in the western part of Bogot\u00e1 and reveals high concentrations of rotavirus. The results suggest that villi from mouse intestines serve as a reliable model for isolating rotavirus from wastewaters. These findings provide a new approach for environmental health surveillance in Colombia, based on molecular epidemiology for waters highly contaminated with human enteric viruses.<\/jats:p>","DOI":"10.7705\/biomedica.7935","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T21:32:05Z","timestamp":1764624725000},"page":"30-40","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogot\u00e1, D. C.","Detecci\u00f3n de fragmentos gen\u00f3micos y evaluaci\u00f3n de la capacidad infecciosa de rotavirus aislados de aguas residuales utilizadas para riego en el occidente de Bogot\u00e1, D. C."],"prefix":"10.7705","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1931-8081","authenticated-orcid":false,"given":"Jos\u00e9 Seir","family":"Jord\u00e1n","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Virus, Facultad de Medicina, Universidad Nacional de Colombia, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2863-6345","authenticated-orcid":false,"given":"Carlos Arturo","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Virus, Facultad de Medicina, Universidad Nacional de Colombia, Bogot\u00e1, D. C., Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"155367","doi-asserted-by":"crossref","unstructured":"Randazzo W, Truchado P, Cuevas-Ferrando E, Sim\u00f3n P, Allende A, S\u00e1nchez G. 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