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El par\u00e1sito Leishmania es fagocitado por los macr\u00f3fagos y se aloja en el fagolisosoma; por consiguiente, se necesita la activaci\u00f3n por los linfocitos CD4-Th1 para controlar la infecci\u00f3n. La presentaci\u00f3n antig\u00e9nica es cr\u00edtica para dicha activaci\u00f3n al presentar secuencias cortas del par\u00e1sito en el bolsillo de las mol\u00e9culas del complejo mayor de histocompatibilidad (CMH) de clase II.Objetivo. Identificar las secuencias lineales de prote\u00ednas inmunog\u00e9nicas de Leishmania (Viannia) spp., mediante herramientas bioinform\u00e1ticas frente a mol\u00e9culas humanas del CMH de clase II.Materiales y m\u00e9todos. Se tomaron secuencias lineales de las prote\u00ednas GP63, HSP70 y PEPCK de Leishmania panamensis y se predijo la uni\u00f3n de secuencias de 15 amino\u00e1cidos frente a diversos HLA-DR\u03b2*04 por medio de herramientas de predicci\u00f3n. Se confirm\u00f3 que las regiones est\u00e1n muy conservadas entre las especies del par\u00e1sito, con nula o poca analog\u00eda con las prote\u00ednas humanas. Finalmente, se analizaron las interacciones de los p\u00e9ptidos con mejores resultados por acoplamiento molecular frente al HLA-DR\u03b2*04:01. Resultados. En la primera fase del estudio, se identificaron seis secuencias con gran potencial de ser ep\u00edtopos T naturales de Leishmania spp. Las secuencias GP63267-282, GP63282-297 y PEPCK535-550 presentaron m\u00faltiples interacciones y bajas energ\u00edas de interacci\u00f3n frente al bolsillo del HLA-DR\u03b2*04:01 y orientaci\u00f3n de N-terminal a C-terminal. Conclusiones. Se identificaron tres secuencias como posibles ep\u00edtopos Th1 de Leishmania spp.; sin embargo, es necesaria la validaci\u00f3n in vitro para confirmar estos hallazgos.<\/jats:p>","DOI":"10.7705\/biomedica.8036","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:09:31Z","timestamp":1788268171000},"page":"421-431","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Estrategia de tamizaje para la selecci\u00f3n de ep\u00edtopes de Leishmania spp en humanos por medio del an\u00e1lisis de interacciones moleculares: un acercamiento desde la bioinform\u00e1tica","Screening strategy for the selection of Leishmania spp. epitopes in humans through molecular interactions analysis: A bioinformatics approach"],"prefix":"10.7705","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5751-9773","authenticated-orcid":false,"given":"Magda Melissa","family":"Fl\u00f3rez","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n, Facultad de Medicina, Universidad Cooperativa de Colombia, Santa Marta, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2311-8339","authenticated-orcid":false,"given":"Juli\u00e1n","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Ciencias de la Vida, Universidad Sim\u00f3n Bol\u00edvar, Barranquilla, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8547-2132","authenticated-orcid":false,"given":"Francy Elaine","family":"Torres","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n, Facultad de Medicina, Universidad Cooperativa de Colombia, Santa Marta, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4444","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"167582","unstructured":"World Health Organization. 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