{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T08:39:40Z","timestamp":1772267980704,"version":"3.50.1"},"reference-count":26,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009331","name":"Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support","doi-asserted-by":"crossref","award":["ZYLX201703"],"award-info":[{"award-number":["ZYLX201703"]}],"id":[{"id":"10.13039\/501100009331","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Orthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In total, 19 orthodontic patients participated in this study. Saliva samples were collected at the following three timepoints: before (T0) and 3 months (T1) and 6 months (T2) after the placement of orthodontic appliances. High-throughput sequencing was performed based on the 16S rRNA gene V4 region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The phyla of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were predominant. Observed Species, Chao1 and ACE, which represent\n                      <jats:italic>\u03b1<\/jats:italic>\n                      diversity, were significantly decreased at T1 and subsequently increased at T2. In addition, the\n                      <jats:italic>\u03b2<\/jats:italic>\n                      diversity at T1 based on the Bray-Curtis distances differed from T0 and T2. The relative abundances of\n                      <jats:italic>Prevotella<\/jats:italic>\n                      ,\n                      <jats:italic>Porphyromonas<\/jats:italic>\n                      and\n                      <jats:italic>Peptostreptococcus<\/jats:italic>\n                      were decreased with treatment, whereas those of\n                      <jats:italic>Capnocytophaga<\/jats:italic>\n                      and\n                      <jats:italic>Neisseria<\/jats:italic>\n                      exhibited the opposite results. In total, 385 of 410 operational taxonomic units were shared at T0, T1 and T2. The co-occurrence networks with hub nodes at T1 were the most complex.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Orthodontic treatment temporarily affected the saliva microbial community. This dynamic alteration in species did not induce deterioration in oral health. Oral hygiene instructions were necessary and should be emphasized during each visit. Further studies with longer observation periods and more participants are required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.10446","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T04:37:25Z","timestamp":1606797445000},"page":"e10446","source":"Crossref","is-referenced-by-count":8,"title":["Salivary microbial changes during the first 6 months of orthodontic treatment"],"prefix":"10.7717","volume":"8","author":[{"given":"Mei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry, School of Stomatology, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, School of Stomatology, Capital Medical University, Beijing, 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