{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:00:27Z","timestamp":1787032827316,"version":"build-2736575974"},"reference-count":40,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research University Project"},{"DOI":"10.13039\/100012527","name":"Office of Higher Education Commission","doi-asserted-by":"crossref","award":["NRU59-023-HR"],"award-info":[{"award-number":["NRU59-023-HR"]}],"id":[{"id":"10.13039\/100012527","id-type":"DOI","asserted-by":"crossref"}]},{"name":"90th Anniversary of Chulalongkorn University, Rachadapisek Sompote Fund"},{"name":"Science Achievement Scholarship of Thailand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Being ubiquitous, fungi are common opportunistic pathogens to humans that can lead to invasive and life-threatening infections in immunocompromised individuals. Eukaryote-resembling cell membrane and filamentous branches make the fungal diagnosis difficult. This study therefore developed a ready-to-use ITS1 loop-mediated isothermal amplification combined with hydroxynaphthol blue (LAMP-HNB) for rapid, sensitive and specific colorimetric detection of universal fungi in all phyla. The ITS1 LAMP-HNB could identify every evolutionary phylum of fungi according to sequence analyses. We tested a total of 30 clinically relevant fungal isolates (representing three major human pathogenic phyla of fungi, namely Zygomycota, Ascomycota and Basidiomycota) and 21 non-fungal isolates, and the ITS1 LAMP-HNB properly identified all isolates, with a detection limit of as low as 4.6 ag (9.6 copies), which was identical to ITS1 and 18S rDNA PCR. The assays were also validated on the feasibility of point-of-care diagnostic with real food (dry peanuts, chili and garlics) and blood samples. Furthermore, the shelf life of our ready-to-use ITS1 LAMP activity (\u226550%) was more than 40 days at 30\u00a0\u00b0C with 3\u20135% polyvinyl alcohol or glycerol additive. The results supported the ready-to-use ITS1 LAMP-HNB for simple detection of fungi contamination with high sensitivity in local and resource-constrained areas to prevent opportunistic fungal species infections.<\/jats:p>","DOI":"10.7717\/peerj.11082","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T05:01:04Z","timestamp":1616043664000},"page":"e11082","source":"Crossref","is-referenced-by-count":15,"title":["Specific and sensitive, ready-to-use universal fungi detection by visual color using ITS1 loop-mediated isothermal amplification combined hydroxynaphthol blue"],"prefix":"10.7717","volume":"9","author":[{"given":"Ilada","family":"Choopara","sequence":"first","affiliation":[{"name":"Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yothin","family":"Teethaisong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, 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