{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:53:24Z","timestamp":1790610804699,"version":"4.1.0"},"reference-count":39,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>Paeonol is a potent therapy for psoriasis. This study aimed to screen out paeonol-targeted genes in psoriasis and validate the potential of using paeonol for the management of psoriasis.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>Microarray datasets were obtained from the Gene Expression Omnibus. The differentially expressed genes (DEGs) in the lesional skin samples and the overlapping genes between DEGs and paeonol- and psoriasis-related genes were defined as potential targets for psoriasis. After being treated with si-ATG5 and pc-ATG5, human HaCaT cells were treated with 100 ng\/ml IL-22 and 10 ng\/ml TNF-<jats:italic>\u03b1<\/jats:italic> with and without paeonol. Cell proliferation, apoptosis, and expression of interleukin (IL)-6, IL-1<jats:italic>\u03b2<\/jats:italic>, Beclin 1, ATG5, and p62 in HaCaT cells were determined using ESLIA, PCR, and Western blot analysis.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>A total of 779 DEGs were identified in the lesional skin samples compared with the non-lesional tissues. The autophagy-related 5 (<jats:italic>ATG5<\/jats:italic>) gene was the only gene that overlapped between the DEGs and genes related to paeonol and psoriasis. Cell proliferation, inflammatory cytokines (IL-6 and IL-1<jats:italic>\u03b2<\/jats:italic>), and ATG5 expression were increased in IL-22\/TNF-<jats:italic>\u03b1<\/jats:italic>-stimulated HaCaT (model) cells compared with control. Paeonol treatment rescued all changes. si-ATG5 transfection increased inflammation and apoptosis in model cells compared with controls. pc-ATG5 prevented IL-22\/TNF-<jats:italic>\u03b1<\/jats:italic>-induced changes in HaCaT cells. Also, si-ATG5 decreased p62 and Beclin 1 proteins, while pc-ATG5 increased them both.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusions<\/jats:title>\n               <jats:p><jats:italic>ATG5<\/jats:italic>-dependent autophagy plays a crucial role in psoriasis. The <jats:italic>ATG5<\/jats:italic> gene might be a therapeutic target for the management of in vitro psoriasis.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.11278","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T08:18:52Z","timestamp":1621930732000},"page":"e11278","source":"Crossref","is-referenced-by-count":9,"title":["The paeonol target gene autophagy-related 5 has a potential therapeutic value in psoriasis treatment"],"prefix":"10.7717","volume":"9","author":[{"given":"Qian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Dermatology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqiao","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Dermatology, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs,Chinese Academy of Medical Science&Peking Union Medical College, Nanjing, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxue","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Foreign Languages, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine and College of Integrated Chinese and Western Medicine, Nanjing, Jiangsu Province, 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