{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:33:14Z","timestamp":1788226394245,"version":"build-2803163510"},"reference-count":105,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T00:00:00Z","timestamp":1620691200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness","award":["CGL2013-41674-P"],"award-info":[{"award-number":["CGL2013-41674-P"]}]},{"name":"Spanish Ministry of Economy and Competitiveness","award":["CGL2016-75590-P"],"award-info":[{"award-number":["CGL2016-75590-P"]}]},{"name":"Spanish Ministry of Economy and Competitiveness","award":["PID2019-110603RB-I00"],"award-info":[{"award-number":["PID2019-110603RB-I00"]}]},{"name":"Spanish Ministry of Economy and Competitiveness","award":["AEI\/FEDER"],"award-info":[{"award-number":["AEI\/FEDER"]}]},{"name":"UE and CSIC Open Access Publication Support Initiative"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Lava caves differ from karstic caves in their genesis and mineral composition. Subsurface microbiology of lava tube caves in Canary Islands, a volcanic archipelago in the Atlantic Ocean, is largely unknown. We have focused the investigation in a representative lava tube cave, Fuente de la Canaria Cave, in La Palma Island, Spain, which presents different types of speleothems and colored microbial mats. Four samples collected in this cave were studied using DNA next-generation sequencing and field emission scanning electron microscopy for bacterial identification, functional profiling, and morphological characterization. The data showed an almost exclusive dominance of\n                    <jats:italic>Bacteria<\/jats:italic>\n                    over\n                    <jats:italic>Archaea<\/jats:italic>\n                    . The distribution in phyla revealed a majority abundance of\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    (37\u201389%), followed by\n                    <jats:italic>Actinobacteria<\/jats:italic>\n                    ,\n                    <jats:italic>Acidobacteria<\/jats:italic>\n                    and\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Rokubacteria. These four phyla comprised a total relative abundance of 72\u201396%. The main ecological functions in the microbial communities were chemoheterotrophy, methanotrophy, sulfur and nitrogen metabolisms, and CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation; although other ecological functions were outlined. Genome annotations of the especially representative taxon Ga0077536 (about 71% of abundance in moonmilk) predicted the presence of genes involved in CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation, formaldehyde consumption, sulfur and nitrogen metabolisms, and microbially-induced carbonate precipitation. The detection of several putative lineages associated with C, N, S, Fe and Mn indicates that Fuente de la Canaria Cave basalts are colonized by metabolically diverse prokaryotic communities involved in the biogeochemical cycling of major elements.\n                  <\/jats:p>","DOI":"10.7717\/peerj.11386","type":"journal-article","created":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T04:14:05Z","timestamp":1620706445000},"page":"e11386","source":"Crossref","is-referenced-by-count":43,"title":["Prokaryotic communities from a lava tube cave in La Palma Island (Spain) are involved in the biogeochemical cycle of major elements"],"prefix":"10.7717","volume":"9","author":[{"given":"Jose Luis","family":"Gonzalez-Pimentel","sequence":"first","affiliation":[{"name":"Laboratorio Hercules, Universidade de Evora, Evora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Martin-Pozas","sequence":"additional","affiliation":[{"name":"Geology, Museo Nacional de Ciencias Naturales, CSIC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valme","family":"Jurado","sequence":"additional","affiliation":[{"name":"Environmental Microbiology, Instituto de Recursos Naturales y Agrobiologia, CSIC, Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Zelia","family":"Miller","sequence":"additional","affiliation":[{"name":"Laboratorio Hercules, Universidade de Evora, Evora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Teresa","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Laboratorio Hercules, Universidade de Evora, Evora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio","family":"Fernandez-Lorenzo","sequence":"additional","affiliation":[{"name":"Grupo de Espeleologia Tebexcorade, La Palma, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Sanchez-Moral","sequence":"additional","affiliation":[{"name":"Geology, Museo Nacional de Ciencias Naturales, CSIC, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesareo","family":"Saiz-Jimenez","sequence":"additional","affiliation":[{"name":"Environmental Microbiology, Instituto de Recursos Naturales y Agrobiologia, CSIC, Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2021,5,11]]},"reference":[{"key":"10.7717\/peerj.11386\/ref-1","doi-asserted-by":"publisher","first-page":"21733","DOI":"10.1038\/s41598-020-78759-4","article-title":"In situ modelling of biofilm formation in a hydrothermal spring cave","volume":"10","author":"Anda","year":"2020","journal-title":"Scientific Reports"},{"key":"10.7717\/peerj.11386\/ref-2","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.resmic.2008.10.001","article-title":"Bacteria and free-living amoeba in Lascaux Cave","volume":"160","author":"Bastian","year":"2009","journal-title":"Research in Microbiology"},{"key":"10.7717\/peerj.11386\/ref-3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/978-3-642-32226-6_11","article-title":"Melting and mantle sources in the Azores","volume-title":"Volcanoes of the azores: revealing the geological secrets of the central Northern Atlantic Islands","author":"Beier","year":"2018"},{"key":"10.7717\/peerj.11386\/ref-4","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.7717\/peerj.11386\/ref-5","article-title":"Effects of natural and anthropogenic impacts on the microbiome of the Indian River Lagoon, Fl, USA","author":"Bradshaw","year":"2020","journal-title":"D. 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