{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T03:38:19Z","timestamp":1782445099545,"version":"3.54.5"},"reference-count":19,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"National Institute of Health Grants","award":["R01AI099527"],"award-info":[{"award-number":["R01AI099527"]}]},{"name":"National Institute of Health Grants","award":["R01AI099473"],"award-info":[{"award-number":["R01AI099473"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.<\/jats:p>","DOI":"10.7717\/peerj.11794","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T10:58:18Z","timestamp":1636455498000},"page":"e11794","source":"Crossref","is-referenced-by-count":5,"title":["Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations"],"prefix":"10.7717","volume":"9","author":[{"given":"Ozkan","family":"Aydemir","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Brown University, Providence, RI, United States of 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