{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T21:29:24Z","timestamp":1775165364934,"version":"3.50.1"},"reference-count":31,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31772887"],"award-info":[{"award-number":["31772887"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Endogenous hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) is generated in many metabolism pathways, and has been recognized as a second messenger against antibiotics and reactive oxygen species (ROS). In\n                    <jats:italic>Aeromonas veronii<\/jats:italic>\n                    , Small Protein B (SmpB) plays an important role in resisting stress. The absence of\n                    <jats:italic>smpB<\/jats:italic>\n                    could trigger sulfate assimilation pathway to adapt the nutrient deficiency, of which was mediated by up-regulation of\n                    <jats:italic>cbl<\/jats:italic>\n                    and\n                    <jats:italic>cys<\/jats:italic>\n                    genes and followed with enhancing H\n                    <jats:sub>2<\/jats:sub>\n                    S production. To figure out the mutual regulations of\n                    <jats:italic>cbl<\/jats:italic>\n                    and\n                    <jats:italic>cys<\/jats:italic>\n                    genes, a series of experiments were performed. Compared with the wild type,\n                    <jats:italic>cysH<\/jats:italic>\n                    was down-regulated significantly in\n                    <jats:italic>cbl<\/jats:italic>\n                    deletion by qRT-PCR. The fluorescence analysis further manifested that Cbl had a positive regulatory effect on the promoter of\n                    <jats:italic>cysJIH<\/jats:italic>\n                    . Bacterial one-hybrid analysis and electrophoretic mobility shift assay (EMSA) verified that Cbl bound with the promoter of\n                    <jats:italic>cysJIH<\/jats:italic>\n                    . Collectively, the tolerance to adversity could be maintained by the production of H\n                    <jats:sub>2<\/jats:sub>\n                    S when SmpB was malfunctioned, of which the activity of\n                    <jats:italic>cysJIH<\/jats:italic>\n                    promoter was positively regulated by upstream Cbl protein. The outcomes also suggested the enormous potentials of\n                    <jats:italic>Aeromonas veronii<\/jats:italic>\n                    in environmental adaptability.\n                  <\/jats:p>","DOI":"10.7717\/peerj.12058","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T04:03:42Z","timestamp":1631160222000},"page":"e12058","source":"Crossref","is-referenced-by-count":2,"title":["Cbl upregulates\n                    <i>cysH<\/i>\n                    for hydrogen sulfide production in\n                    <i>Aeromonas veronii<\/i>"],"prefix":"10.7717","volume":"9","author":[{"given":"Yidong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zebin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqiong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongqian","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"issue":"1","key":"10.7717\/peerj.12058\/ref-1","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.bbrc.2015.01.058","article-title":"CysB-dependent 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