{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:34:43Z","timestamp":1790728483625,"version":"4.1.0"},"reference-count":52,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T00:00:00Z","timestamp":1638835200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"KKU Research Fund","award":["Project no. I62-00-19-03"],"award-info":[{"award-number":["Project no. I62-00-19-03"]}]},{"name":"Graduate School, Khon Kaen University Research Grant","award":["611JH107"],"award-info":[{"award-number":["611JH107"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Vancomycin-resistant enterococci (VRE), especially\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    , have been a global concern, often causing serious healthcare-associated infections. We established a rapid approach for detecting\n                    <jats:italic>E. faecium<\/jats:italic>\n                    and vancomycin-resistance genes (\n                    <jats:italic>vanA<\/jats:italic>\n                    and\n                    <jats:italic>vanB<\/jats:italic>\n                    ) in clinical samples using isothermal recombinase polymerase amplification (RPA) combined with a lateral-flow (LF) strip. Specific RPA primer sets and probes for\n                    <jats:italic>ddl<\/jats:italic>\n                    (to identify the presence of\n                    <jats:italic>E. faecium<\/jats:italic>\n                    )\n                    <jats:italic>vanA<\/jats:italic>\n                    and\n                    <jats:italic>vanB<\/jats:italic>\n                    genes were designed. The RPA reaction was performed under isothermal condition at 37\u00a0\u00b0C within 20 min and read using the LF strip within a further 5 min. A total of 141 positive blood-cultures and 136 stool\/rectal swab samples were tested using RPA-LF method compared to the conventional PCR method. The RPA-LF method exhibited 100% sensitivity in both blood-culture (60\n                    <jats:italic>E. faecium<\/jats:italic>\n                    ; 35\n                    <jats:italic>vanA<\/jats:italic>\n                    type and two\n                    <jats:italic>vanB<\/jats:italic>\n                    type) and stool\/rectal-swab samples (63\n                    <jats:italic>E. faecium<\/jats:italic>\n                    and 36\n                    <jats:italic>vanA<\/jats:italic>\n                    type) without cross-reaction (100% specificity). The lower detection limit of the RPA-LF was approximately 10 times better than that of the conventional PCR method. The RPA-LF method is an alternative rapid method with excellent sensitivity and specificity for detecting\n                    <jats:italic>E. faecium<\/jats:italic>\n                    ,\n                    <jats:italic>vanA<\/jats:italic>\n                    , and\n                    <jats:italic>vanB<\/jats:italic>\n                    , and it has the potential to be used as a point-of-care device for VRE therapy and prevention.\n                  <\/jats:p>","DOI":"10.7717\/peerj.12561","type":"journal-article","created":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T06:01:47Z","timestamp":1638856907000},"page":"e12561","source":"Crossref","is-referenced-by-count":13,"title":["Rapid detection of\n                    <i>Enterococcus<\/i>\n                    and vancomycin resistance using recombinase polymerase amplification"],"prefix":"10.7717","volume":"9","author":[{"given":"Pimchanok","family":"Panpru","sequence":"first","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpasiri","family":"Srisrattakarn","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuttanun","family":"Panthasri","sequence":"additional","affiliation":[{"name":"The 8th Regional Medical Science Center, Udon Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharaporn","family":"Tippayawat","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aroonwadee","family":"Chanawong","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratree","family":"Tavichakorntrakool","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jureerut","family":"Daduang","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumyai","family":"Wonglakorn","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aroonlug","family":"Lulitanond","sequence":"additional","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2021,12,7]]},"reference":[{"key":"10.7717\/peerj.12561\/ref-1","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1520\/JFS13607J","article-title":"Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification","volume":"39","author":"Akane","year":"1994","journal-title":"Journal of Forensic Sciences"},{"key":"10.7717\/peerj.12561\/ref-2","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1186\/1743-422X-10-184","article-title":"A cost effective real-time PCR for the detection of adenovirus from viral swabs","volume":"10","author":"Al-Siyabi","year":"2013","journal-title":"Virology Journal"},{"key":"10.7717\/peerj.12561\/ref-3","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1128\/JCM.38.1.345-350.2000","article-title":"Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR","volume":"38","author":"Al-Soud","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"10.7717\/peerj.12561\/ref-4","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1017\/ice.2016.254","article-title":"Incidence and outcomes associated with infections caused by vancomycin-resistant Enterococci in the United States: systematic literature review and meta-analysis","volume":"38","author":"Chiang","year":"2017","journal-title":"Infection Control and Hospital Epidemiology"},{"key":"10.7717\/peerj.12561\/ref-5","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.jhin.2005.02.016","article-title":"Guidelines for the control of glycopeptide-resistant enterococci in hospitals","volume":"62","author":"Cookson","year":"2006","journal-title":"The Journal of Hospital Infection"},{"key":"10.7717\/peerj.12561\/ref-6","doi-asserted-by":"publisher","first-page":"2565","DOI":"10.1021\/ac403750z","article-title":"Nucleic acid test to diagnose cryptosporidiosis: lab assessment in animal and patient specimens","volume":"86","author":"Crannell","year":"2014","journal-title":"Analytical Chemistry"},{"key":"10.7717\/peerj.12561\/ref-7","doi-asserted-by":"publisher","first-page":"e112146","DOI":"10.1371\/journal.pone.0112146","article-title":"Equipment-free incubation of recombinase polymerase amplification reactions using body heat","volume":"9","author":"Crannell","year":"2014","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.12561\/ref-8","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1373\/clinchem.2013.213504","article-title":"Isothermal recombinase polymerase amplification assay applied to the detection of group B streptococci in vaginal\/anal samples","volume":"60","author":"Daher","year":"2014","journal-title":"Clinical Chemistry"},{"key":"10.7717\/peerj.12561\/ref-9","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1086\/430909","article-title":"Comparison of mortality associated with vancomycin-resistant and vancomycin-susceptible enterococcal bloodstream infections: a meta-analysis","volume":"41","author":"DiazGranados","year":"2005","journal-title":"Clinical Infectious Diseases"},{"key":"10.7717\/peerj.12561\/ref-10","doi-asserted-by":"publisher","first-page":"2352","DOI":"10.1093\/jac\/dky209","article-title":"MALDI-TOF for the rapid detection of carbapenemase-producing Enterobacteriaceae: comparison of the commercialized MBT STAR\u00ae-Carba IVD Kit with two inhouse MALDI-TOF techniques and the RAPIDEC\u00ae CARBA NP","volume":"73","author":"Dortet","year":"2018","journal-title":"The Journal of Antimicrobial Chemotherapy"},{"key":"10.7717\/peerj.12561\/ref-11","doi-asserted-by":"publisher","first-page":"2296","DOI":"10.1007\/s12161-018-1200-7","article-title":"Rapid detection of Staphylococcus aureus via recombinase polymerase amplification combined with lateral flow strip","volume":"11","author":"Du","year":"2018","journal-title":"Food Analytical Methods"},{"key":"10.7717\/peerj.12561\/ref-12","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1111\/1750-3841.14078","article-title":"Recombinase polymerase amplification combined with lateral flow strip for Listeria monocytogenes detection in food","volume":"83","author":"Du","year":"2018","journal-title":"Journal of Food Science"},{"key":"10.7717\/peerj.12561\/ref-13","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1128\/jcm.33.1.24-27.1995","article-title":"Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR","volume":"33","author":"Dutka-Malen","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"10.7717\/peerj.12561\/ref-14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2005.06.026","article-title":"The role and application of enterococci in food and health","volume":"106","author":"Foulqui\u00e9\u00a0Moreno","year":"2006","journal-title":"International Journal of Food Microbiology"},{"key":"10.7717\/peerj.12561\/ref-15","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1128\/AEM.69.1.312-319.2003","article-title":"Effect of raw-milk cheese consumption on the enterococcal flora of human feces","volume":"69","author":"Gelsomino","year":"2003","journal-title":"Applied and Environmental Microbiology"},{"key":"10.7717\/peerj.12561\/ref-16","doi-asserted-by":"publisher","first-page":"421","DOI":"10.4161\/viru.21282","article-title":"Intrinsic and acquired resistance mechanisms in Enterococcus","volume":"3","author":"Hollenbeck","year":"2012","journal-title":"Virulence"},{"key":"10.7717\/peerj.12561\/ref-17","doi-asserted-by":"publisher","first-page":"37732","DOI":"10.1038\/srep37732","article-title":"Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay","volume":"6","author":"Jauset-Rubio","year":"2016","journal-title":"Scientific Reports"},{"key":"10.7717\/peerj.12561\/ref-18","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/j.mayocp.2012.01.007","article-title":"Current concepts in laboratory testing to guide antimicrobial therapy","volume":"87","author":"Jenkins","year":"2012","journal-title":"Mayo Clinic Proceedings"},{"key":"10.7717\/peerj.12561\/ref-19","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1128\/AEM.71.3.1135-1141.2005","article-title":"Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors","volume":"71","author":"Jiang","year":"2005","journal-title":"Applied and Environmental Microbiology"},{"key":"10.7717\/peerj.12561\/ref-20","doi-asserted-by":"publisher","first-page":"2791","DOI":"10.1007\/s00705-017-3399-9","article-title":"Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars","volume":"162","author":"Kapoor","year":"2017","journal-title":"Archives of Virology"},{"key":"10.7717\/peerj.12561\/ref-21","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1186\/1475-2875-13-99","article-title":"Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis","volume":"13","author":"Kersting","year":"2014","journal-title":"Malaria Journal"},{"key":"10.7717\/peerj.12561\/ref-22","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3402\/mehd.v12i2.8104","article-title":"Culture-based knowledge on biodiversity, development and stability of human gastrointestinal microflora","volume":"12","author":"Kleessen","year":"2000","journal-title":"Microbial Ecology in Health and Disease"},{"key":"10.7717\/peerj.12561\/ref-23","article-title":"Enterococcal infection treatment and antibiotic resistance","volume-title":"Enterococci: from commensals to leading causes of drug resistant infection","author":"Kristich","year":"2014"},{"key":"10.7717\/peerj.12561\/ref-24","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/s12985-016-0504-8","article-title":"Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics","volume":"13","author":"Londo\u00f1o","year":"2016","journal-title":"Virology Journal"},{"key":"10.7717\/peerj.12561\/ref-25","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s42483-021-00089-8","article-title":"Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay","volume":"3","author":"Lu","year":"2021","journal-title":"Phytopathology Research"},{"key":"10.7717\/peerj.12561\/ref-26","doi-asserted-by":"publisher","first-page":"421","DOI":"10.2217\/fvl-2017-0034","article-title":"Recombinase polymerase amplification: a promising point-of-care detection method for enteric viruses","volume":"12","author":"Moore","year":"2017","journal-title":"Future Virology"},{"key":"10.7717\/peerj.12561\/ref-27","doi-asserted-by":"publisher","first-page":"2443","DOI":"10.1128\/JCM.36.9.2443-2446.1998","article-title":"Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis","volume":"36","author":"Morata","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"10.7717\/peerj.12561\/ref-28","article-title":"Antimicrobial resistance 2000-2020","author":"National Antimicrobial Resistance Surveillance Center\u00a0Thailand","year":"2020"},{"key":"10.7717\/peerj.12561\/ref-29","doi-asserted-by":"publisher","first-page":"e0213416","DOI":"10.1371\/journal.pone.0213416","article-title":"Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay","volume":"14","author":"Peng","year":"2019","journal-title":"PLOS One"},{"key":"10.7717\/peerj.12561\/ref-30","doi-asserted-by":"publisher","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLOS Biology"},{"key":"10.7717\/peerj.12561\/ref-31","doi-asserted-by":"publisher","first-page":"e0207811","DOI":"10.1371\/journal.pone.0207811","article-title":"Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test","volume":"13","author":"Qi","year":"2018","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.12561\/ref-32","doi-asserted-by":"publisher","first-page":"e27544v1","DOI":"10.7287\/peerj.preprints.27544v1","article-title":"Recombinase polymerase amplification (RPA) versus PCR for ancient DNA library amplification","volume":"7","author":"Richards","year":"2019","journal-title":"PeerJ Preprints"},{"key":"10.7717\/peerj.12561\/ref-33","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1186\/s13071-015-1055-3","article-title":"Isothermal recombinase polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection","volume":"8","author":"Rosser","year":"2015","journal-title":"Parasites & Vectors"},{"key":"10.7717\/peerj.12561\/ref-34","doi-asserted-by":"publisher","first-page":"e0004638","DOI":"10.1371\/journal.pntd.0004638","article-title":"An innovative field-applicable molecular test to diagnose cutaneous Leishmania Viannia spp. infections","volume":"10","author":"Saldarriaga","year":"2016","journal-title":"PLOS Neglected Tropical Diseases"},{"key":"10.7717\/peerj.12561\/ref-35","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1111\/tbed.13126","article-title":"Development of a rapid and sensitive recombinase polymerase amplification-lateral flow assay for detection of Burkholderia mallei","volume":"66","author":"Saxena","year":"2019","journal-title":"Transboundary and Emerging Diseases"},{"key":"10.7717\/peerj.12561\/ref-36","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","article-title":"PCR inhibitors-occurrence, properties and removal","volume":"113","author":"Schrader","year":"2012","journal-title":"Journal of Applied Microbiology"},{"key":"10.7717\/peerj.12561\/ref-37","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1111\/jcpt.13383","article-title":"Prevalence of vancomycin-resistant enterococci in Asia-A systematic review and meta-analysis","volume":"46","author":"Shrestha","year":"2021","journal-title":"Journal of Clinical Pharmacy and Therapeutics"},{"key":"10.7717\/peerj.12561\/ref-38","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.ab.2018.01.019","article-title":"Rapid detection of potyviruses from crude plant extracts","volume":"546","author":"Silva","year":"2018","journal-title":"Analytical Biochemistry"},{"key":"10.7717\/peerj.12561\/ref-39","article-title":"Predictive value of blood culture time to positivity","author":"Smith","year":"2018"},{"key":"10.7717\/peerj.12561\/ref-40","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1007\/s11274-020-02938-8","article-title":"Direct detection of methicillin-resistant in Staphylococcus spp. in positive blood culture by isothermal recombinase polymerase amplification combined with lateral flow dipstick assay","volume":"36","author":"Srisrattakarn","year":"2020","journal-title":"World Journal of Microbiology & Biotechnology"},{"key":"10.7717\/peerj.12561\/ref-41","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1128\/9781555816728.ch21","article-title":"Enterococcus","volume-title":"Manual of clinical microbiology","author":"Teixeira","year":"2011","edition":"10"},{"key":"10.7717\/peerj.12561\/ref-42","article-title":"TwistAmp\u00ae manuals","author":"TwistDx\u2122\u00a0Limited","year":"2018"},{"key":"10.7717\/peerj.12561\/ref-43","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1111\/jph.12616","article-title":"Rapid detection of Candidatus Phytoplasma mali by recombinase polymerase amplification assays","volume":"165","author":"Valasevich","year":"2017","journal-title":"Journal of Phytopathology"},{"key":"10.7717\/peerj.12561\/ref-44","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.ijfoodmicro.2005.08.027","article-title":"Enterocin A production by Enterococcus faecium FAIR-E 406 is characterised by a temperature- and pH-dependent switch-off mechanism when growth is limited due to nutrient depletion","volume":"107","author":"Vanden\u00a0Berghe","year":"2006","journal-title":"International Journal of Food Microbiology"},{"key":"10.7717\/peerj.12561\/ref-45","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1007\/s00705-015-2738-y","article-title":"Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification","volume":"161","author":"Wang","year":"2016","journal-title":"Archives of Virology"},{"key":"10.7717\/peerj.12561\/ref-46","doi-asserted-by":"publisher","first-page":"1288","DOI":"10.1017\/ice.2016.174","article-title":"Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2011-2014","volume":"37","author":"Weiner","year":"2016","journal-title":"Infection Control and Hospital Epidemiology"},{"key":"10.7717\/peerj.12561\/ref-47","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.3389\/fmicb.2020.01015","article-title":"A recombinase polymerase amplification and lateral flow strip combined method that detects Salmonella enterica Serotype Typhimurium with no worry of primer-dependent artifacts","volume":"11","author":"Wu","year":"2020","journal-title":"Frontiers in Microbiology"},{"key":"10.7717\/peerj.12561\/ref-48","doi-asserted-by":"publisher","first-page":"3551","DOI":"10.1007\/s00436-016-5120-4","article-title":"Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle feces","volume":"115","author":"Wu","year":"2016","journal-title":"Parasitology Research"},{"key":"10.7717\/peerj.12561\/ref-49","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.vetpar.2017.02.019","article-title":"Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions","volume":"237","author":"Yin","year":"2017","journal-title":"Veterinary Parasitology"},{"key":"10.7717\/peerj.12561\/ref-50","doi-asserted-by":"publisher","first-page":"3321","DOI":"10.1002\/j.1460-2075.1985.tb04083.x","article-title":"T4 phage gene uvsX product catalyzes homologous DNA pairing","volume":"4","author":"Yonesaki","year":"1985","journal-title":"The EMBO Journal"},{"key":"10.7717\/peerj.12561\/ref-51","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1186\/s13756-017-0231-y","article-title":"Incidence, clinical characteristics, and outcomes of nosocomial Enterococcus spp. bloodstream infections in a tertiary-care hospital in Beijing, China: a four-year retrospective study","volume":"6","author":"Zhang","year":"2017","journal-title":"Antimicrobial Resistance and Infection Control"},{"key":"10.7717\/peerj.12561\/ref-52","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.jviromet.2014.06.026","article-title":"Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(\u00ae) using reverse transcription-recombinase polymerase amplification","volume":"207","author":"Zhang","year":"2014","journal-title":"Journal of Virological Methods"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/12561.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/12561.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/12561.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/12561.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T09:27:12Z","timestamp":1673947632000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/12561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,7]]},"references-count":52,"alternative-id":["10.7717\/peerj.12561"],"URL":"https:\/\/doi.org\/10.7717\/peerj.12561","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.7287\/peerj.12561v0.1\/reviews\/1","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.12561v0.1\/reviews\/2","asserted-by":"object"},{"id-type":"doi","id":"10.7287\/peerj.12561v0.2\/reviews\/1","asserted-by":"object"}]},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,7]]},"article-number":"e12561"}}