{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:56:42Z","timestamp":1792144602582,"version":"4.4.0"},"reference-count":41,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T00:00:00Z","timestamp":1649116800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Scientific Research Foundation of Guangdong Province","award":["A2020106"],"award-info":[{"award-number":["A2020106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Evidence has demonstrated that puerarin is a potential medicine for the treatment of cardiac hypertrophy. However, the precise underlying molecular mechanisms of the protective effect of puerarin are still unclear. Here, we aimed to explore the regulatory mechanisms of lncRNAs\/mRNAs co-expression network in a cardiac hypertrophy mouse model after puerarin treatment.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>A mouse model of cardiac hypertrophy was established by transverse aortic constriction (TAC). The echocardiography, tissue staining and western blot were used to examine the protective effect of puerarin. Then RNA sequencing (RNA-seq) was carried out to analyze systematically mRNAs and lncRNAs expression. The target lncRNA were confirmed using qRT-PCR. Moreover, a coding\/non-coding gene co-expression network were established to find the interaction of lncRNA and mRNAs. The biological process, cellular component, molecular function and pathways of different expression mRNAs targeted by lncRNA were explored using Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Puerarin exhibited an obvious inhibitory effect in cardiac hypertrophy in TAC model. RNA-seq analysis was performed to investigate the lncRNAs and mRNAs expression patterns of cardiomyocytes in sham and TAC groups treated with or without puerarin. RNA-seq identified that TAC downregulated four lncRNAs, which could be revised by puerarin treatment (|log2 Fold change| &gt; 2 and FDR &lt; 0.05). Among them, expression alterations of lncRNA Airn (antisense of Igf2r non-protein coding RNA) was confirmed by qRT-PCR. Pearson\u2019s correlation coefficients of co-expression levels suggested that there was an interactive relationship between Airn and 2,387 mRNAs (<jats:italic>r<\/jats:italic> &gt; 0.95 or <jats:italic>r<\/jats:italic> &lt; \u22120.95). Those co-expressed mRNAs were enriched in some important biological processes such as translational initiation, cell proliferation, insulin-like growth factor binding and poly(A) RNA binding. KEGG analyses suggested that those Airn-interacted mRNAs were enriched in endocytosis, signaling pathways regulating pluripotency of stem cells and the Jak-STAT pathway.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Puerarin may exert beneficial effects on cardiac hypertrophy through regulating the lncRNAs\/mRNAs co-expression network.<\/jats:p>\n<\/jats:sec>","DOI":"10.7717\/peerj.13144","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T09:36:24Z","timestamp":1649151384000},"page":"e13144","source":"Crossref","is-referenced-by-count":11,"title":["RNA sequencing reveals novel LncRNA\/mRNAs co-expression network associated with puerarin-mediated inhibition of cardiac hypertrophy in mice"],"prefix":"10.7717","volume":"10","author":[{"given":"Shan","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Cardiology, Laboratory of Heart Center, Guangdong Provincial Biomedical Engineering Technology Research Center for Cardiovascular Disease, Sino-Japanese Cooperation Platform for Translational Research in Heart Failure, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China"},{"name":"Department of Geriatrics, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiwen","family":"Ou","sequence":"additional","affiliation":[{"name":"Dongguan Hospital of Southern Medical University, Dongguan, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Laboratory of Heart Center, Guangdong Provincial 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