{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:19:44Z","timestamp":1782951584934,"version":"3.54.5"},"reference-count":27,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Increase and Decrease Projects at the Central Level of China","award":["2060302"],"award-info":[{"award-number":["2060302"]}]},{"name":"Science and Technology Innovation Special Project of Hubei Province","award":["2019ACA119"],"award-info":[{"award-number":["2019ACA119"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pinellia ternata<\/jats:italic>\n                      (Thunb.), a perennial herbal plant in the Araceae family, has great medicinal value and market demand. In August 2020, an outbreak of severe leaf spot blight disease resulted in a huge yield loss of\n                      <jats:italic>P. ternata<\/jats:italic>\n                      . It is necessary to isolate and identify the pathogens that cause spot blight on\n                      <jats:italic>P. ternata<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, we isolated and identified the pathogens by fulfilling Koch\u2019s postulates. Disease samples with typical spot blight symptoms were collected and pathogens were isolated from the diseased tissues. The pathogen was identified based on its biological characteristics and molecular analysis of internal transcribed (rDNA-ITS) and large subunit (LSU) sequences. Phylogenetic tree were constructed using MEGA7 software and pathogenicity tests were performed using\n                      <jats:italic>in vivo<\/jats:italic>\n                      inoculation. Finally, the pathogen was recovered and identified from the inoculated plants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Based on Koch\u2019s postulates, we identified the pathogen causing spot blight on\n                      <jats:italic>P. ternata<\/jats:italic>\n                      as\n                      <jats:italic>Stagonosporopsis cucurbitacearum<\/jats:italic>\n                      . To our knowledge, this is the first study to explore spot blight on\n                      <jats:italic>P. ternata<\/jats:italic>\n                      caused by\n                      <jats:italic>S. cucurbitacearum<\/jats:italic>\n                      in China.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.13278","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T04:12:36Z","timestamp":1649823156000},"page":"e13278","source":"Crossref","is-referenced-by-count":2,"title":["Identifying and characterizing\n                    <i>Stagonosporopsis cucurbitacearum<\/i>\n                    causing spot blight on\n                    <i>Pinellia ternata<\/i>\n                    in China"],"prefix":"10.7717","volume":"10","author":[{"given":"Jia","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaohuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bisheng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhuan","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2022,4,13]]},"reference":[{"issue":"12","key":"10.7717\/peerj.13278\/ref-1","doi-asserted-by":"publisher","first-page":"371","DOI":"10.2307\/2475702","article-title":"Notes 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