{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T16:08:23Z","timestamp":1702570103251},"reference-count":22,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T00:00:00Z","timestamp":1651536000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81925014","31771657","31900594"],"award-info":[{"award-number":["81925014","31771657","31900594"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Project of Jiangsu Province the Natural Science Fund","award":["BK20190651"],"award-info":[{"award-number":["BK20190651"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>It has been thought that oocyte may develop in a low oxygen environment, as changes in follicle structure and formation of a fluid-filled antrum. The survival of hypoxic tissues is controlled by hypoxia-inducible factors (HIFs) that are activated in a low oxygen state. HIF1<jats:italic>\u03b1<\/jats:italic> is expressed in mature mouse oocytes and continues to be expressed after fertilization, from the 2-cell to blastocyst stage. However, the physiological roles of HIF pathway during oogenesis and embryogenesis have still not been elucidated in detail.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>Mutant mice with oocyte-specific HIF1<jats:italic>\u03b1<\/jats:italic> deletion were generated by crossing <jats:italic>Hif<\/jats:italic>1<jats:italic>\u03b1<\/jats:italic><jats:sup><jats:italic>fl<\/jats:italic>\/<jats:italic>fl<\/jats:italic><\/jats:sup> mice with transgenic mice expressing <jats:italic>Gdf9<\/jats:italic>-promoter-mediated Cre recombinase. Breeding assay was carried out to detect female fertility. <jats:italic>In vitro<\/jats:italic> fertilization and embryo culture were used to assess early embryo development. Oocyte meiotic progression was also examined. Quantitative RT-PCR was used for analyzing of candidate genes expression.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>We successfully generated mutant mice with oocyte-specific deletion of HIF1<jats:italic>\u03b1<\/jats:italic>. Oocytes loss of HIF1<jats:italic>\u03b1<\/jats:italic> did not affect female fertility, ovulation and early embryo development. Moreover, oocytes can mature in vitro, and form well-organized spindle in the absence of HIF1<jats:italic>\u03b1<\/jats:italic>. In addition, pronounced differences in <jats:italic>Hif2\u03b1<\/jats:italic> and <jats:italic>Hif3\u03b1<\/jats:italic> mRNA expression were not observed in HIF1<jats:italic>\u03b1<\/jats:italic>-deleted oocytes. These results revealed that HIF pathway in oocytes is not essential for female fertility.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.13370","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T07:16:21Z","timestamp":1651562181000},"page":"e13370","source":"Crossref","is-referenced-by-count":2,"title":["HIF1<i>\u03b1<\/i> is dispensable for oocyte development and female fertility in mice"],"prefix":"10.7717","volume":"10","author":[{"given":"Yujia","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Suzhou Municipal Hospital, Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyu","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Suzhou Municipal Hospital, Nanjing Medical University, Nanjing, 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