{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T16:34:17Z","timestamp":1774888457128,"version":"3.50.1"},"reference-count":46,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Council of Thailand (NRCT) under Mid-career Researcher grant","award":["RSA6180042"],"award-info":[{"award-number":["RSA6180042"]}]},{"name":"The Royal Golden Jubilee Ph.D. Program","award":["PHD\/0227\/2560"],"award-info":[{"award-number":["PHD\/0227\/2560"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    is a major cause of nosocomial infection, and the incidence of extensively drug-resistant\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    (XDRAB) infections has dramatically increased worldwide. In this study, we aimed to explore the complete genome sequence of XDRAB 329, ST1166\/98 (Oxford\/Pasteur), which is an outbreak clone from a hospital in Thailand. Whole-genome sequencing (WGS) was performed using short-read Illumina and long-read PacBio sequencing, and a conjugation assay of its plasmid was performed. The complete genome sequence of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB329 revealed a circular chromosome 3,948,038 bp in length with 39% GC content. Antibiotic resistance genes (ARGs), including beta-lactam resistance (\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-51<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>ADC-25<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-23<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-1D)<\/jats:sub>\n                    , aminoglycoside resistance (\n                    <jats:italic>aph(3\u2032)<\/jats:italic>\n                    -Ia,\n                    <jats:italic>aph<\/jats:italic>\n                    (3\u2033)-Ib,\n                    <jats:italic>aph<\/jats:italic>\n                    (6)-Id,\n                    <jats:italic>arm<\/jats:italic>\n                    A), tetracycline resistance (\n                    <jats:italic>tet<\/jats:italic>\n                    (B),\n                    <jats:italic>tet<\/jats:italic>\n                    (R)), macrolide resistance (\n                    <jats:italic>mph<\/jats:italic>\n                    (E),\n                    <jats:italic>msr<\/jats:italic>\n                    (E)), and efflux pumps, were found. Mobile genetic elements (MGEs) analysis of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB329 revealed two plasmids (pAB329a and pAB329b), three prophages, 19 genomic islands (GIs), and 33 insertion sequences (ISs). pAB329a is a small circular plasmid of 8,731 bp, and pAB329b is a megaplasmid of 82,120 bp.\n                    <jats:italic>aph<\/jats:italic>\n                    (3\u2032)-VIa was detected in pAB329b, and a major facilitator superfamily (MFS) transporter was detected in the prophage\n                    <jats:italic>. Acinetobacter baumannii<\/jats:italic>\n                    resistance island 4 (AbaR4) harboring tetracycline and aminoglycoside resistance was detected in the genome of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB329. pAB329b, which belongs to Rep-type GR6 (plasmid lineage LN_1), is a conjugative plasmid with the ability to transfer an aminoglycoside resistance gene to sodium azide-resistant\n                    <jats:italic>A. baumannii.<\/jats:italic>\n                    This study provides insights into the features of the MGEs of XDRAB\n                    <jats:italic>,<\/jats:italic>\n                    which are the main reservoir and source of dissemination of ARGs.\n                  <\/jats:p>","DOI":"10.7717\/peerj.13718","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T03:47:41Z","timestamp":1657684061000},"page":"e13718","source":"Crossref","is-referenced-by-count":9,"title":["Insights into mobile genetic elements and the role of conjugative plasmid in transferring aminoglycoside resistance in extensively drug-resistant\n                    <i>Acinetobacter baumannii<\/i>\n                    AB329"],"prefix":"10.7717","volume":"10","author":[{"given":"Supat","family":"Khongfak","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rapee","family":"Thummeepak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udomluk","family":"Leungtongkam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kannipa","family":"Tasanapak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aunchalee","family":"Thanwisai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sutthirat","family":"Sitthisak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, Thailand"},{"name":"Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Muang,  Phitsanulok, 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