{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:06:26Z","timestamp":1788404786823,"version":"build-2803163510"},"reference-count":55,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of National Natural Science Foundationof China","award":["82104921"],"award-info":[{"award-number":["82104921"]}]},{"name":"Science and Technology Innovation Project of the Chinese Academy of Traditional Chinese Medicine","award":["CI2021A02405"],"award-info":[{"award-number":["CI2021A02405"]}]},{"name":"Project of Guang\u2019anmen Hospital, China Academy of Traditional Chinese Medicine","award":["Y2019-14"],"award-info":[{"award-number":["Y2019-14"]}]},{"name":"Project of Hebei Administration of Traditional Chinese Medicine","award":["2018077"],"award-info":[{"award-number":["2018077"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Objective<\/jats:title>\n               <jats:p>Shenling Baizhu powder (SBP) has been shown to reverse the abnormal expression of the aromatic hydrocarbon receptor (AHR) mediated by air pollution. Our study aimed to understand the main ingredient of SBP and investigate its action mechanism in preventing polycystic ovary syndrome (POCS) and postmenopausal osteoporosis (PMO).<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>The active ingredients of SBP with the highest binding affinity to AHR were screened using a Chinese medicine database, and their binding mechanism was simulated using molecular dynamics simulation (MDS). Rutin was utilized to treat ovarian granulosa cell lines and osteoblast cell lines. The cell lines were treated with a gradient of rutin concentration (0.01 mmol\/L, 0.05 mmol\/L and 0.1 mmol\/L) to find the optimal drug dose. PCR was used to detect AHR and apoptosis-related proteins, and WB to detect the expression of AHR, caspase-3 and cleaved-caspase-3. Finally, the CCK-8 cell proliferation assay detected the proliferation of cells.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>We obtained Rutin through the Chinese medicine database, and dynamics simulation determined its binding sites. Ovarian granulosa cell lines and osteoblast cell lines were treated with Rutin. RT-PCR and western blotting revealed that the expression of apoptosis-associated protein Bcl-2 was elevated, and the expression of AHR, Bax, caspase-3 and PARP were decreased. CCK-8 results showed accelerated proliferation in both cell types.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusion<\/jats:title>\n               <jats:p>Rutin, the main ingredient of SBP compound, works by binding to AHR, which can improve POCS and PMO by inhibiting cell apoptosis and by promoting cell proliferation.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.13939","type":"journal-article","created":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T08:14:25Z","timestamp":1666944865000},"page":"e13939","source":"Crossref","is-referenced-by-count":7,"title":["Prevention of polycystic ovary syndrome and postmenopausal osteoporosis by inhibiting apoptosis with Shenling Baizhu powder compound"],"prefix":"10.7717","volume":"10","author":[{"given":"Jing","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai-li","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-yu","family":"Ma","sequence":"additional","affiliation":[{"name":"Hebei General Hospital, Department of Traditional Chinese Medicine, Hebei, Chinese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-hua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-yu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Guang\u2019anmen South Area Hospital, China Academy of Chinese Medical Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-yan","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Characteristic Medical Center of Chinese People\u2019s Armed Police Force, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-qiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Characteristic Medical Center of 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