{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T08:46:58Z","timestamp":1772268418619,"version":"3.50.1"},"reference-count":40,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Humanities and Social Science Research Youth Fund Project of Mini-try of Education","award":["20YJCZH253"],"award-info":[{"award-number":["20YJCZH253"]}]},{"name":"Child Development Research Institute of Jiaxing University","award":["20PY3-1"],"award-info":[{"award-number":["20PY3-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>We explored the mechanism underlying exercise-mediated placental angiogenesis and perinatal outcome using mouse models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Three-week-old C57BL\/6 female mice were randomly divided into four experimental groups: standard-chow diet (SC), standard chow diet + exercise (SC-Ex), high-fat diet (HFD), and high-fat diet + exercise (HFD-Ex). After 13 weeks of exercise intervention, the male and female mice were caged. Approximately six to seven pregnant female mice from each experimental group were randomly selected for body composition, qRT-PCR, histological, and western blot analysis. The remaining mice were allowed to deliver naturally, and the perinatal outcome indexes were observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Rusults<\/jats:title>\n                    <jats:p>\n                      The results showed that exercise intervention significantly improved the body composition and glucose tolerance in HFD-fed pregnant mice. The HFD group showed adipocyte infiltration, placental local hypoxia, and villous vascular thrombosis with a significant (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05) increase in the expression of VEGF and ANGPT1 proteins. Exercise intervention significantly elevated the expression of PPAR\n                      <jats:italic>\u03b3<\/jats:italic>\n                      , alleviated hypoxia and inflammation-related conditions, and inhibited angiogenesis. sFlt-1 mRNA in HFD group was significantly higher than that in SC group (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Furthermore, the HFD significantly reduced (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05) the fertility rate in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Thus, HFD aggravates placental inflammation and the hypoxic environment and downregulates the expression of PPAR\n                      <jats:italic>\u03b3<\/jats:italic>\n                      and PPAR\n                      <jats:italic>\u03b1<\/jats:italic>\n                      in the placenta. However, exercise intervention can significantly alleviate these conditions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.14562","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T03:50:22Z","timestamp":1676865022000},"page":"e14562","source":"Crossref","is-referenced-by-count":5,"title":["Effects of swimming before and during pregnancy on placental angiogenesis and perinatal outcome in high-fat diet-fed mice"],"prefix":"10.7717","volume":"11","author":[{"given":"Xiaofeng","family":"Zhu","sequence":"first","affiliation":[{"name":"Child Development Research Institute of Jiaxing University, Jiaxing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwei","family":"Chen","sequence":"additional","affiliation":[{"name":"Child Development Research Institute of Jiaxing University, Jiaxing, 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