{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T14:48:47Z","timestamp":1746802127757,"version":"3.37.3"},"reference-count":64,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gansu Local Pig Germplasm Resources","award":["GSLK-2021-13"],"award-info":[{"award-number":["GSLK-2021-13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Breast carcinoma amplified sequence 2 (<jats:italic>BCAS2<\/jats:italic>) participates in pre-mRNA splicing and DNA damage response, which is implicated in spermatogenesis and meiosis initiation in mouse. Nevertheless, the physiological roles of <jats:italic>BCAS2<\/jats:italic> in the testes of large mammals especially boars remain largely unknown.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>In this study, testes were collected from Hezuo pig at three development stages including 30 days old (30 d), 120 days old (120 d), and 240 days old (240 d). <jats:italic>BCAS2<\/jats:italic> CDS region was firstly cloned using RT-PCR method, and its molecular characteristics were identified using relevant bioinformatics software. Additionally, the expression patterns and cellular localization of BCAS2 were analyzed by quantitative real-time PCR (qRT-PCR), Western blot, immunohistochemistry and immunofluorescence.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The cloning and sequence analysis indicated that the Hezuo pig <jats:italic>BCAS2<\/jats:italic> CDS fragment encompassed 678 bp open reading frame (ORF) capable of encoding 225 amino acid residues, and possessed high identities with some other mammals. The results of qRT-PCR and Western blot displayed that BCAS2 levels both mRNA and protein were age-dependent increased (<jats:italic>p<\/jats:italic> &lt; 0.01). Additionally, immunohistochemistry and immunofluorescence results revealed that BCAS2 protein was mainly observed in nucleus of gonocytes at 30 d testes as well as nucleus of spermatogonia and Sertoli cells at 120 and 240 d testes. Accordingly, we conclude that <jats:italic>BCAS2<\/jats:italic> is critical for testicular development and spermatogenesis of Hezuo pig, perhaps by regulating proliferation or differentiation of gonocytes, pre-mRNA splicing of spermatogonia and functional maintenance of Sertoli cells, but specific mechanism still requires be further investigated.<\/jats:p>\n<\/jats:sec>","DOI":"10.7717\/peerj.16341","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T08:09:08Z","timestamp":1698134948000},"page":"e16341","source":"Crossref","is-referenced-by-count":5,"title":["Molecular characterization, expression patterns and cellular localization of <i>BCAS2<\/i> gene in male Hezuo pig"],"prefix":"10.7717","volume":"11","author":[{"given":"Yuran","family":"Tang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haixia","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8701-0647","authenticated-orcid":true,"given":"Zunqiang","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoli","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9467-3406","authenticated-orcid":true,"given":"Xiaoyu","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangbao","family":"Gun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China"},{"name":"Gansu Research Center for Swine Production Engineering and Technology, Lanzhou, Gansu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"issue":"3","key":"10.7717\/peerj.16341\/ref-1","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1093\/molbev\/mss273","article-title":"Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme","volume":"30","author":"Agashe","year":"2013","journal-title":"Molecular Biology and Evolution"},{"issue":"23","key":"10.7717\/peerj.16341\/ref-2","doi-asserted-by":"publisher","first-page":"6569","DOI":"10.1093\/emboj\/19.23.6569","article-title":"Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry","volume":"19","author":"Ajuh","year":"2000","journal-title":"The EMBO Journal"},{"issue":"9\u201310","key":"10.7717\/peerj.16341\/ref-3","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1007\/s00114-017-1505-1","article-title":"Histological analysis and identification of spermatogenesis-related genes in 2-, 6-, and 12-month-old sheep testes","volume":"104","author":"Bai","year":"2017","journal-title":"Naturwissenschaften"},{"issue":"12","key":"10.7717\/peerj.16341\/ref-4","doi-asserted-by":"publisher","first-page":"6226","DOI":"10.1210\/en.2008-0349","article-title":"Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes","volume":"149","author":"Basciani","year":"2008","journal-title":"Endocrinology"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-5","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1146\/annurev.biochem.72.121801.161720","article-title":"Mechanisms of alternative pre-messenger RNA splicing","volume":"72","author":"Black","year":"2003","journal-title":"Annual Review of Biochemistry"},{"issue":"5","key":"10.7717\/peerj.16341\/ref-6","doi-asserted-by":"publisher","first-page":"671","DOI":"10.2144\/000112800","article-title":"The evolution of tools for protein phosphorylation site analysis: from discovery to clinical application","volume":"44","author":"Bonilla","year":"2008","journal-title":"BioTechniques"},{"issue":"33","key":"10.7717\/peerj.16341\/ref-7","doi-asserted-by":"publisher","first-page":"25411","DOI":"10.1074\/jbc.M001676200","article-title":"NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry","volume":"275","author":"Boudrez","year":"2000","journal-title":"Journal of Biological Chemistry"},{"issue":"6","key":"10.7717\/peerj.16341\/ref-8","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1586\/14789450.4.6.711","article-title":"Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin","volume":"4","author":"Cao","year":"2007","journal-title":"Expert Review of Proteomics"},{"issue":"5","key":"10.7717\/peerj.16341\/ref-9","doi-asserted-by":"publisher","first-page":"R149","DOI":"10.1530\/REP-15-0073","article-title":"Linking transcriptomics and proteomics in spermatogenesis","volume":"150","author":"Chalmel","year":"2015","journal-title":"Reproduction"},{"issue":"35","key":"10.7717\/peerj.16341\/ref-10","doi-asserted-by":"publisher","first-page":"31190","DOI":"10.1074\/jbc.M505060200","article-title":"The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation","volume":"280","author":"Chan","year":"2005","journal-title":"Journal of Biological Chemistry"},{"issue":"5643","key":"10.7717\/peerj.16341\/ref-11","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1126\/science.1086602","article-title":"The Prp19p-associated complex in spliceosome activation","volume":"302","author":"Chan","year":"2003","journal-title":"Science"},{"issue":"4","key":"10.7717\/peerj.16341\/ref-12","doi-asserted-by":"publisher","first-page":"2196","DOI":"10.1128\/MCB.18.4.2196","article-title":"Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p","volume":"18","author":"Chen","year":"1998","journal-title":"Molecular and Cellular Biology"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-13","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1261\/rna.034835.112","article-title":"BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing","volume":"19","author":"Chen","year":"2013","journal-title":"RNA"},{"issue":"10","key":"10.7717\/peerj.16341\/ref-14","doi-asserted-by":"publisher","first-page":"5406","DOI":"10.1073\/pnas.96.10.5406","article-title":"Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing","volume":"96","author":"Chen","year":"1999","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"6","key":"10.7717\/peerj.16341\/ref-15","doi-asserted-by":"crossref","first-page":"e0130706","DOI":"10.1371\/journal.pone.0130706","article-title":"BCAS2 regulates delta-notch signaling activity through delta pre-mrna splicing in drosophila wing development","volume":"10","author":"Chou","year":"2015","journal-title":"PLOS ONE"},{"issue":"1\u20132","key":"10.7717\/peerj.16341\/ref-16","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.mce.2007.04.010","article-title":"Expression of 11beta-hydroxysteroid-dehydrogenase 2 in Sertoli cells of boar testes","volume":"272","author":"Claus","year":"2007","journal-title":"Molecular and Cellular Endocrinology"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/bdrc.20142","article-title":"Gonocytes, the forgotten cells of the germ cell lineage","volume":"87","author":"Culty","year":"2009","journal-title":"Birth Defects Research Part C: Embryo Today"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-18","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1095\/biolreprod.113.110544","article-title":"Gonocytes, from the Fifties to the present: is there a reason to change the name?","volume":"89","author":"Culty","year":"2013","journal-title":"Biology of Reproduction"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-19","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1111\/age.12124","article-title":"Identification of species-specific novel transcripts in pig reproductive tissues using RNA-seq","volume":"45","author":"Du","year":"2014","journal-title":"Animal Blood Groups and Biochemical Genetics"},{"issue":"7","key":"10.7717\/peerj.16341\/ref-20","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1534\/g3.115.018382","article-title":"Identification of reproduction-related gene polymorphisms using whole transcriptome sequencing in the large white pig population","volume":"5","author":"Fischer","year":"2015","journal-title":"G3-Genes Genomes Genetics"},{"issue":"5","key":"10.7717\/peerj.16341\/ref-21","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.1074\/mcp.M112.020123","article-title":"Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis","volume":"12","author":"Gan","year":"2013","journal-title":"Molecular & Cellular Proteomics"},{"issue":"4","key":"10.7717\/peerj.16341\/ref-22","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1006\/scdb.1998.0203","article-title":"The central role of Sertoli cells in spermatogenesis","volume":"9","author":"Griswold","year":"1998","journal-title":"Seminars in Cell & Developmental Biology"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-23","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1093\/biolre\/ioy027","article-title":"50 years of spermatogenesis: Sertoli cells and their interactions with germ cells","volume":"99","author":"Griswold","year":"2018","journal-title":"Biology of Reproduction"},{"issue":"9","key":"10.7717\/peerj.16341\/ref-24","doi-asserted-by":"publisher","first-page":"2105","DOI":"10.1128\/MCB.01505-09","article-title":"Molecular architecture of the human Prp19\/CDC5L complex","volume":"30","author":"Grote","year":"2010","journal-title":"Molecular and Cellular Biology"},{"issue":"4","key":"10.7717\/peerj.16341\/ref-25","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1002\/pro.3790","article-title":"Bridging protein structure, dynamics, and function using hydro-gen\/deuterium-exchange mass spectrometry","volume":"29","author":"Hodge","year":"2020","journal-title":"Protein Science"},{"issue":"9","key":"10.7717\/peerj.16341\/ref-26","doi-asserted-by":"publisher","first-page":"e74851","DOI":"10.1371\/journal.pone.0074851","article-title":"The Prp19 complex directly functions in mitotic spindle assembly","volume":"8","author":"Hofmann","year":"2013","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.16341\/ref-27","doi-asserted-by":"publisher","first-page":"34927","DOI":"10.1038\/srep34927","article-title":"Conditional knockout of breast carcinoma amplified sequence 2 (BCAS2) in mouse forebrain causes dendritic malformation via beta-catenin","volume":"6","author":"Huang","year":"2016","journal-title":"Scientific Reports"},{"issue":"3","key":"10.7717\/peerj.16341\/ref-28","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1002\/1097-0029(20000801)50:3<184::AID-JEMT2>3.0.CO;2-H","article-title":"TNF alpha and the TNF receptor superfamily: structure-function relationship(s)","volume":"50","author":"Idriss","year":"2000","journal-title":"Microscopy Research and Technique"},{"issue":"5\u20136","key":"10.7717\/peerj.16341\/ref-29","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1017\/S0016672308009518","article-title":"Genetic variability maintained in a finite population due to mutational production of neutral and nearly neutral isoalleles","volume":"89","author":"Kimura","year":"2007","journal-title":"Genetical Research"},{"key":"10.7717\/peerj.16341\/ref-30","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1146\/annurev.bi.65.070196.002055","article-title":"The structure and function of proteins involved in mammalian pre-mRNA splicing","volume":"65","author":"Kr\u00e4mer","year":"1996","journal-title":"Annual Review of Biochemistry"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-31","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1038\/bjc.2014.603","article-title":"BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability","volume":"112","author":"Kuo","year":"2015","journal-title":"British Journal of Cancer"},{"issue":"23","key":"10.7717\/peerj.16341\/ref-32","doi-asserted-by":"publisher","first-page":"8877","DOI":"10.1158\/0008-5472.CAN-09-2023","article-title":"Breast cancer amplified sequence 2, a novel negative regulator of the p53 tumor suppressor","volume":"69","author":"Kuo","year":"2009","journal-title":"Cancer Research"},{"issue":"20","key":"10.7717\/peerj.16341\/ref-33","doi-asserted-by":"publisher","first-page":"4071","DOI":"10.1007\/s00018-019-03201-6","article-title":"Mechanisms regulating mammalian spermatogenesis and fertility recovery following germ cell depletion","volume":"76","author":"La","year":"2019","journal-title":"Cellular and Molecular Life Sciences"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-34","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3892\/or.2017.6064","article-title":"miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4\/BCAS2","volume":"39","author":"Lang","year":"2018","journal-title":"Oncology Reports"},{"key":"10.7717\/peerj.16341\/ref-35","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.biocel.2015.08.016","article-title":"BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination","volume":"68","author":"Liao","year":"2015","journal-title":"The International Journal of Biochemistry & Cell Biology"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-36","doi-asserted-by":"publisher","first-page":"14182","DOI":"10.1038\/ncomms14182","article-title":"BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis","volume":"8","author":"Liu","year":"2017","journal-title":"Nature Communications"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-37","doi-asserted-by":"publisher","first-page":"11463","DOI":"10.1038\/s41598-019-47711-6","article-title":"Population genomics analysis revealed origin and high-altitude adaptation of Tibetan pigs","volume":"9","author":"Ma","year":"2019","journal-title":"Scientific Reports"},{"issue":"13","key":"10.7717\/peerj.16341\/ref-38","doi-asserted-by":"publisher","first-page":"1654","DOI":"10.1080\/15384101.2020.1762314","article-title":"Cyclin A2 is essential for mouse gonocyte maturation","volume":"19","author":"Ma","year":"2020","journal-title":"Cell Cycle"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-39","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/S0304-3835(02)00286-0","article-title":"Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer","volume":"185","author":"Maass","year":"2002","journal-title":"Cancer Letters"},{"key":"10.7717\/peerj.16341\/ref-40","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/S0065-3233(05)72008-8","article-title":"Thermodynamics of alpha-helix formation","volume":"72","author":"Makhatadze","year":"2005","journal-title":"Virus Structure"},{"issue":"24","key":"10.7717\/peerj.16341\/ref-41","doi-asserted-by":"publisher","first-page":"8898","DOI":"10.1073\/pnas.84.24.8898","article-title":"Helix stabilization by Glu-...Lys+ salt bridges in short peptides of de novo design","volume":"84","author":"Marqusee","year":"1987","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"11","key":"10.7717\/peerj.16341\/ref-42","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1038\/s41419-022-05432-1","article-title":"The role of SPATA2 in TNF signaling, cancer, and spermatogenesis","volume":"13","author":"Masola","year":"2022","journal-title":"Cell Death & Disease"},{"issue":"1\u20132","key":"10.7717\/peerj.16341\/ref-43","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/S0304-3835(99)00091-9","article-title":"Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines","volume":"140","author":"Nagasaki","year":"1999","journal-title":"Cancer Letters"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-44","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1038\/1700","article-title":"Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex","volume":"20","author":"Neubauer","year":"1998","journal-title":"Nature Genetics"},{"issue":"8","key":"10.7717\/peerj.16341\/ref-45","doi-asserted-by":"publisher","first-page":"151800","DOI":"10.1016\/j.acthis.2021.151800","article-title":"Helix-loop-helix protein ID4 expressed in bovine Sertoli cells","volume":"123","author":"Park","year":"2021","journal-title":"Acta Histochemica"},{"issue":"3","key":"10.7717\/peerj.16341\/ref-46","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1095\/biolreprod.114.126250","article-title":"Combining RNA and protein profiling data with network interactions identifies genes associated with spermatogenesis in mouse and human","volume":"92","author":"Petit","year":"2015","journal-title":"Biology of Reproduction"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-47","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.gene.2012.06.068","article-title":"Pyruvate dehydrogenase complex: mRNA and protein expression patterns of E1\u03b1 subunit genes in human spermatogenesis","volume":"506","author":"Pinheiro","year":"2012","journal-title":"Gene"},{"issue":"81","key":"10.7717\/peerj.16341\/ref-48","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.mod.2018.01.001","article-title":"NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes","volume":"149","author":"Pui","year":"2018","journal-title":"Mechanisms of Development"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-49","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.bbrc.2004.12.187","article-title":"Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2","volume":"328","author":"Qi","year":"2005","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"10","key":"10.7717\/peerj.16341\/ref-50","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1007\/s13258-017-0576-x","article-title":"Strand-specific RNA sequencing in pig testes identifies developmentally regulated genes and circular RNAs","volume":"39","author":"Ran","year":"2017","journal-title":"Genes Genomics"},{"issue":"4","key":"10.7717\/peerj.16341\/ref-51","doi-asserted-by":"publisher","first-page":"966","DOI":"10.3390\/ijms20040966","article-title":"BCAS2 enhances carcinogenic effects of estrogen receptor alpha in breast cancer cells","volume":"20","author":"Salmer\u00f3n-Hern\u00e1ndez","year":"2019","journal-title":"International Journal of Molecular Sciences"},{"issue":"3","key":"10.7717\/peerj.16341\/ref-52","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1095\/biolreprod65.3.771","article-title":"Nature of the spermatogenic arrest in Dazl -\/- mice","volume":"65","author":"Schrans-Stassen","year":"2001","journal-title":"Biology of Reproduction"},{"issue":"7915","key":"10.7717\/peerj.16341\/ref-53","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1038\/s41586-022-04823-w","article-title":"Synonymous mutations in representative yeast genes are mostly strongly non-neutral","volume":"606","author":"Shen","year":"2022","journal-title":"Nature"},{"issue":"3","key":"10.7717\/peerj.16341\/ref-54","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.tig.2017.12.009","article-title":"The definition of open reading frame revisited","volume":"34","author":"Sieber","year":"2018","journal-title":"Trends in Genetics"},{"issue":"10","key":"10.7717\/peerj.16341\/ref-55","doi-asserted-by":"publisher","first-page":"6619","DOI":"10.1074\/jbc.M113.543439","article-title":"The PSO4 protein complex associates with replication protein A (RPA) and modulates the activation of ataxia telangiectasia-mutated and Rad3-related (ATR)","volume":"289","author":"Wan","year":"2014","journal-title":"Journal of Biological Chemistry"},{"issue":"18","key":"10.7717\/peerj.16341\/ref-56","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1071\/RD20215","article-title":"Wild-type p53-induced phosphatase 1 (WIP1) regulates the proliferation of swine Sertoli cells through P53","volume":"32","author":"Wang","year":"2020","journal-title":"Reproduction, Fertility and Development"},{"issue":"9","key":"10.7717\/peerj.16341\/ref-57","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1007\/BF00964926","article-title":"A kinetic analysis of the release of acidic amino acids from rat cortical synaptosomes following pre-loading with 14C glutamic acid","volume":"8","author":"Wheeler","year":"1983","journal-title":"Neurochemical Research"},{"issue":"5","key":"10.7717\/peerj.16341\/ref-58","doi-asserted-by":"publisher","first-page":"e13000","DOI":"10.1111\/cpr.13000","article-title":"RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes","volume":"54","author":"Xia","year":"2021","journal-title":"Cell Proliferation"},{"issue":"22","key":"10.7717\/peerj.16341\/ref-59","doi-asserted-by":"publisher","first-page":"3943","DOI":"10.1242\/dev.129841","article-title":"Maternal BCAS2 protects genomic integrity in mouse early embryonic development","volume":"142","author":"Xu","year":"2015","journal-title":"Development"},{"issue":"2","key":"10.7717\/peerj.16341\/ref-60","doi-asserted-by":"publisher","first-page":"e21187","DOI":"10.1096\/fj.202001035RRR","article-title":"Characterization of sheep spermatogenesis through single-cell RNA sequencing","volume":"35","author":"Yang","year":"2021","journal-title":"The FASEB Journal"},{"issue":"3","key":"10.7717\/peerj.16341\/ref-61","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1093\/biolre\/ioy088","article-title":"Comprehensive analysis of long noncoding RNA and mRNA expression patterns in sheep testicular maturation","volume":"99","author":"Yang","year":"2018","journal-title":"Biology of Reproduction"},{"issue":"8","key":"10.7717\/peerj.16341\/ref-62","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1182\/blood-2018-09-876599","article-title":"BCAS2 is essential for hematopoietic stem and progenitor cell maintenance during zebrafish embryogenesis","volume":"133","author":"Yu","year":"2019","journal-title":"Blood"},{"issue":"1","key":"10.7717\/peerj.16341\/ref-63","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/s40104-021-00638-3","article-title":"Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis","volume":"12","author":"Zhang","year":"2021","journal-title":"Journal of Animal Science and Biotechnology"},{"issue":"4","key":"10.7717\/peerj.16341\/ref-64","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1080\/10495398.2018.1521825","article-title":"H2AFZ, RNF4 and NR4A1 loci are associated with boar semen quality by population association studies","volume":"30","author":"Zhao","year":"2019","journal-title":"Animal Biotechnology"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/16341.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16341.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16341.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16341.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T08:09:32Z","timestamp":1698134972000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/16341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,24]]},"references-count":64,"alternative-id":["10.7717\/peerj.16341"],"URL":"https:\/\/doi.org\/10.7717\/peerj.16341","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{},"ISSN":["2167-8359"],"issn-type":[{"type":"electronic","value":"2167-8359"}],"subject":[],"published":{"date-parts":[[2023,10,24]]},"article-number":"e16341"}}