{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:00:40Z","timestamp":1787032840380,"version":"build-2736575974"},"reference-count":44,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency, Thailand","doi-asserted-by":"crossref","award":["#P18-50116"],"award-info":[{"award-number":["#P18-50116"]}],"id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency, Thailand","doi-asserted-by":"crossref","award":["#P2052577"],"award-info":[{"award-number":["#P2052577"]}],"id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      possesses a cobalamin-dependent methionine synthase (MS). MS is putatively encoded by the PF3D7_1233700 gene, which is orthologous and syntenic in\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      . However, its vulnerability as an antimalarial target has not been assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We edited the PF3D7_1233700 and PF3D7_0417200 (dihydrofolate reductase-thymidylate synthase, DHFR-TS) genes and obtained transgenic\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      parasites expressing epitope-tagged target proteins under the control of the\n                      <jats:italic>glmS<\/jats:italic>\n                      ribozyme. Conditional loss-of-function mutants were obtained by treating transgenic parasites with glucosamine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DHFR-TS, but not MS mutants showed a significant proliferation defect over 96 h, suggesting that\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      MS is not a vulnerable antimalarial target.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.16595","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T03:46:28Z","timestamp":1705290388000},"page":"e16595","source":"Crossref","is-referenced-by-count":2,"title":["Antimalarial target vulnerability of the putative\n                    <i>Plasmodium falciparum<\/i>\n                    methionine synthase"],"prefix":"10.7717","volume":"12","author":[{"given":"Nirut","family":"Leela","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parichat","family":"Prommana","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumalee","family":"Kamchonwongpaisan","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tana","family":"Taechalertpaisarn","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of 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