{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T12:22:35Z","timestamp":1781612555706,"version":"3.54.5"},"reference-count":64,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T00:00:00Z","timestamp":1707436800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Outstanding Young Talent Fund of Traditional Chinese Medicine","award":["2021ZQ043"],"award-info":[{"award-number":["2021ZQ043"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Objective<\/jats:title>\n               <jats:p>Cerebral infarction is the main cause of death in patients with cerebrovascular diseases. Our research aimed to screen and validate pyroptosis-related genes in cerebral infarction for the targeted therapy of cerebral infarction.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods and results<\/jats:title>\n               <jats:p>A total of 1,517 differentially expressed genes (DEGs) were obtained by DESeq2 software analysis. Gene set enrichment analysis results indicated that genes of middle cerebral artery occlusion (MCAO) mice aged 3 months and 18 months were enriched in pyroptosis, respectively. Differentially expressed pyroptosis-related genes (including Aim2, Casp8, Gsdmd, Naip2, Naip5, Naip6 and Trem2) were obtained through intersection of DEGs and genes from pyroptosis Gene Ontology Term (GO:0070269), and they were up-regulated in the brain tissues of MCAO mice in <jats:uri xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE137482\">GSE137482<\/jats:uri>. In addition, Casp8, Gsdmd, and Trem2 were verified to be significantly up-regulated in MCAO mice in <jats:uri xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE93376\">GSE93376<\/jats:uri>. The evaluation of neurologic function and triphenyltetrazolium chloride staining showed that the MCAO mouse models were successfully constructed. Meanwhile, the expressions of TNF-<jats:italic>\u03b1<\/jats:italic>, pyroptosis-related proteins, Casp8, Gsdmd and Trem2 in MCAO mice were significantly up-regulated. We selected Trem2 for subsequent functional analysis. OGD treatment of BV2 cell <jats:italic>in vitro<\/jats:italic> significantly upregulated the expressions of Trem2. Subsequent downregulation of Trem2 expression in OGD-BV2 cells further increased the level of pyroptosis. Therefore, Trem2 is a protective factor regulating pyroptosis, thus influencing the progression of cerebral infarction.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusions<\/jats:title>\n               <jats:p>Casp8, Gsdmd and Trem2 can regulate pyroptosis, thus affecting cerebral infarction.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.16818","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T09:13:45Z","timestamp":1707470025000},"page":"e16818","source":"Crossref","is-referenced-by-count":7,"title":["Study on pyroptosis-related genes Casp8, Gsdmd and Trem2 in mice with cerebral infarction"],"prefix":"10.7717","volume":"12","author":[{"given":"Shunli","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"},{"name":"The Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linsheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xilin","family":"Xin","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongbo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2024,2,9]]},"reference":[{"key":"10.7717\/peerj.16818\/ref-1","doi-asserted-by":"publisher","first-page":"e92648","DOI":"10.1371\/journal.pone.0092648","article-title":"Evidence of trem2 variant associated with triple risk of Alzheimer\u2019s disease","volume":"9","author":"Abduljaleel","year":"2014","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.16818\/ref-2","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/jcbfm.2013.236","article-title":"Pyroptotic neuronal cell death mediated by the AIM2 inflammasome","volume":"34","author":"Adamczak","year":"2014","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"key":"10.7717\/peerj.16818\/ref-3","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.it.2017.01.003","article-title":"Recent insights into the molecular mechanisms underlying pyroptosis and gasdermin family functions","volume":"38","author":"Aglietti","year":"2017","journal-title":"Trends in Immunology"},{"key":"10.7717\/peerj.16818\/ref-4","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1111\/bpa.12476","article-title":"A brain in flame; do inflammasomes and pyroptosis influence stroke pathology?","volume":"27","author":"Barrington","year":"2017","journal-title":"Brain Pathology"},{"key":"10.7717\/peerj.16818\/ref-5","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1084\/jem.20022220","article-title":"Impaired differentiation of osteoclasts in TREM-2-deficient individuals","volume":"198","author":"Cella","year":"2003","journal-title":"Journal of Experimetnal Medicine"},{"issue":"5","key":"10.7717\/peerj.16818\/ref-6","doi-asserted-by":"publisher","first-page":"a036392","DOI":"10.1101\/cshperspect.a036392","article-title":"Recent insights on inflammasomes, gasdermin pores, and pyroptosis","volume":"12","author":"De\u00a0Vasconcelos","year":"2020","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"key":"10.7717\/peerj.16818\/ref-7","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1038\/nature18590","article-title":"Pore-forming activity and structural autoinhibition of the gasdermin family","volume":"535","author":"Ding","year":"2016","journal-title":"Nature"},{"key":"10.7717\/peerj.16818\/ref-8","doi-asserted-by":"publisher","first-page":"109595","DOI":"10.1016\/j.biopha.2019.109595","article-title":"Pyroptosis: a new frontier in cancer","volume":"121","author":"Fang","year":"2020","journal-title":"Biomedicine and Pharmacotherapy"},{"key":"10.7717\/peerj.16818\/ref-9","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1038\/s41586-019-1770-6","article-title":"Caspase-8 is the molecular switch for apoptosis, necroptosis and pyroptosis","volume":"575","author":"Fritsch","year":"2019","journal-title":"Nature"},{"key":"10.7717\/peerj.16818\/ref-10","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1016\/j.bbamcr.2009.01.002","article-title":"Role of BNIP3 in TNF-induced cell death\u2013TNF upregulates BNIP3 expression","volume":"1793","author":"Ghavami","year":"2009","journal-title":"Biochimica et Biophysica Acta\/General Subjects"},{"key":"10.7717\/peerj.16818\/ref-11","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1161\/STR.0b013e3181fcb238","article-title":"Guidelines for the primary prevention of stroke: a guideline for healthcare professionals from the American Heart Association\/American Stroke Association","volume":"42","author":"Goldstein","year":"2011","journal-title":"Stroke"},{"key":"10.7717\/peerj.16818\/ref-12","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-021-00817-w","article-title":"Pyroptosis in stroke-new insights into disease mechanisms and therapeutic strategies","author":"Gou","year":"2021","journal-title":"Journal of Physiology and Biochemistry"},{"key":"10.7717\/peerj.16818\/ref-13","doi-asserted-by":"publisher","first-page":"363","DOI":"10.2527\/1990.682363x","article-title":"Effects of dietary amines on small intestinal variables in neonatal pigs fed soy protein isolate","volume":"68","author":"Grant","year":"1990","journal-title":"Journal of Animal Science"},{"key":"10.7717\/peerj.16818\/ref-14","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1097\/FJC.0000000000000550","article-title":"Low-dose Sinapic Acid Abates the Pyroptosis of Macrophages by Downregulation of lncRNA-MALAT1 in Rats With Diabetic Atherosclerosis","volume":"71","author":"Han","year":"2018","journal-title":"Journal of Cardiovascular Pharmacology"},{"key":"10.7717\/peerj.16818\/ref-15","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1016\/j.molcel.2017.01.021","article-title":"The TRAIL-induced cancer secretome promotes a tumor-supportive immune microenvironment via CCR2","volume":"65","author":"Hartwig","year":"2017","journal-title":"Molecular Cell"},{"key":"10.7717\/peerj.16818\/ref-16","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1016\/j.molcel.2017.01.022","article-title":"Caspase-8 acts in a non-enzymatic role as a scaffold for assembly of a pro-inflammatory FADDosome complex upon TRAIL stimulation","volume":"65","author":"Henry","year":"2017","journal-title":"Molecular Cell"},{"key":"10.7717\/peerj.16818\/ref-17","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/s41590-020-0669-6","article-title":"FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation","volume":"21","author":"Hu","year":"2020","journal-title":"Nature Immunology"},{"key":"10.7717\/peerj.16818\/ref-18","doi-asserted-by":"publisher","first-page":"109315","DOI":"10.1016\/j.intimp.2022.109315","article-title":"Edaravone dexborneol provides neuroprotective benefits by suppressing NLRP3 inflammasome-induced microglial pyroptosis in experimental ischemic stroke","volume":"113","author":"Hu","year":"2022","journal-title":"International Immunopharmacology"},{"key":"10.7717\/peerj.16818\/ref-19","doi-asserted-by":"publisher","first-page":"101534","DOI":"10.1016\/j.redox.2020.101534","article-title":"Metformin protects against intestinal ischemia-reperfusion injury and cell pyroptosis via TXNIP-NLRP3-GSDMD pathway","volume":"32","author":"Jia","year":"2020","journal-title":"Redox Biology"},{"key":"10.7717\/peerj.16818\/ref-20","doi-asserted-by":"publisher","first-page":"2949","DOI":"10.1038\/npp.2014.164","article-title":"Upregulation of TREM2 ameliorates neuropathology and rescues spatial cognitive impairment in a transgenic mouse model of Alzheimer\u2019s disease","volume":"39","author":"Jiang","year":"2014","journal-title":"Neuropsychopharmacology"},{"key":"10.7717\/peerj.16818\/ref-21","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1007\/s12035-013-8424-8","article-title":"TREM2 in Alzheimer\u2019s disease","volume":"48","author":"Jiang","year":"2013","journal-title":"Molecular Neurobiology"},{"key":"10.7717\/peerj.16818\/ref-22","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1111\/imr.12287","article-title":"Pyroptotic cell death defends against intracellular pathogens","volume":"265","author":"Jorgensen","year":"2015","journal-title":"Immunological Reviews"},{"key":"10.7717\/peerj.16818\/ref-23","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1089\/ther.2013.0020","article-title":"Triggering receptor expressed on myeloid cells-2 correlates to hypothermic neuroprotection in ischemic stroke","volume":"3","author":"Kawabori","year":"2013","journal-title":"Therapeutic Hypothermia and Temperature Management"},{"key":"10.7717\/peerj.16818\/ref-24","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling","volume":"526","author":"Kayagaki","year":"2015","journal-title":"Nature"},{"issue":"1","key":"10.7717\/peerj.16818\/ref-25","doi-asserted-by":"publisher","first-page":"3788","DOI":"10.1038\/s41598-018-21998-3","article-title":"ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages","volume":"8","author":"Lee","year":"2018","journal-title":"Scientific Reports"},{"key":"10.7717\/peerj.16818\/ref-26","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/j.bbrc.2019.03.202","article-title":"Caspase-1 inhibition mediates neuroprotection in experimental stroke by polarizing M2 microglia\/macrophage and suppressing NF-kappaB activation","volume":"513","author":"Li","year":"2019","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"10.7717\/peerj.16818\/ref-27","doi-asserted-by":"publisher","first-page":"717644","DOI":"10.3389\/fnagi.2021.717644","article-title":"Enriched environment attenuates pyroptosis to improve functional recovery after cerebral Ischemia\/Reperfusion injury","volume":"13","author":"Liu","year":"2021","journal-title":"Frontiers in Aging Neuroscience"},{"key":"10.7717\/peerj.16818\/ref-28","doi-asserted-by":"publisher","first-page":"2254","DOI":"10.1038\/s41380-020-0698-4","article-title":"Hippocampal TNF-death receptors, caspase cell death cascades, and IL-8 in alcohol use disorder","volume":"26","author":"Liu","year":"2021","journal-title":"Molecular Psychiatry"},{"key":"10.7717\/peerj.16818\/ref-29","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1161\/01.str.20.1.84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"Longa","year":"1989","journal-title":"Stroke"},{"key":"10.7717\/peerj.16818\/ref-30","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/S0278-5846(03)00022-8","article-title":"Apoptosis and brain ischaemia","volume":"27","author":"Love","year":"2003","journal-title":"Progress in Neuro-Psychopharmacology and Biological Psychiatry"},{"key":"10.7717\/peerj.16818\/ref-31","doi-asserted-by":"publisher","first-page":"3207","DOI":"10.1002\/jcp.29268","article-title":"Emerging insights into molecular mechanisms underlying pyroptosis and functions of inflammasomes in diseases","volume":"235","author":"Lu","year":"2020","journal-title":"Journal of Cellular Physiology"},{"key":"10.7717\/peerj.16818\/ref-32","doi-asserted-by":"publisher","first-page":"6454","DOI":"10.3390\/ijms21186454","article-title":"Neuroinflammatory mechanisms in ischemic stroke: focus on cardioembolic stroke, background, and therapeutic approaches","volume":"21","author":"Maida","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"10.7717\/peerj.16818\/ref-33","doi-asserted-by":"publisher","first-page":"e1\u2013e7","DOI":"10.1055\/s-0040-1722610","article-title":"Cerebral infarction in immune thrombotic thrombocytopenic purpura is associated with old age, hypertension, smoking, and Anti-ADAMTS13 Ig, but not with mortality","volume":"5","author":"Memon","year":"2021","journal-title":"TH Open"},{"key":"10.7717\/peerj.16818\/ref-34","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s13024-015-0040-9","article-title":"TREM2 in CNS homeostasis and neurodegenerative disease","volume":"10","author":"Painter","year":"2015","journal-title":"Molecular Neurodegeneration"},{"key":"10.7717\/peerj.16818\/ref-35","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.bbi.2018.09.001","article-title":"Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke","volume":"75","author":"Poh","year":"2019","journal-title":"Brain, Behavior, and Immunity"},{"key":"10.7717\/peerj.16818\/ref-36","doi-asserted-by":"publisher","first-page":"e46\u2013e110","DOI":"10.1161\/STR.0000000000000158","article-title":"2018 Guidelines for the early management of patients with acute ischemic stroke: a guideline for healthcare professionals from the american heart association\/american stroke association","volume":"49","author":"Powers","year":"2018","journal-title":"Stroke"},{"key":"10.7717\/peerj.16818\/ref-37","doi-asserted-by":"publisher","first-page":"9743280","DOI":"10.1155\/2017\/9743280","article-title":"NLRP3 inflammasome activation-mediated pyroptosis aggravates myocardial ischemia\/reperfusion injury in diabetic rats","volume":"2017","author":"Qiu","year":"2017","journal-title":"Oxidative Medicine and Cellular Longevity"},{"key":"10.7717\/peerj.16818\/ref-38","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.3389\/fimmu.2018.01121","article-title":"Triggering receptors expressed on myeloid cells 2 promotes corneal resistance against pseudomonas aeruginosa by inhibiting Caspase-1-Dependent pyroptosis","volume":"9","author":"Qu","year":"2018","journal-title":"Frontiers in Immunology"},{"key":"10.7717\/peerj.16818\/ref-39","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.1007\/s12325-017-0622-8","article-title":"Sepsis: a review of advances in management","volume":"34","author":"Rello","year":"2017","journal-title":"Advances in Therapy"},{"key":"10.7717\/peerj.16818\/ref-40","doi-asserted-by":"publisher","first-page":"e10888\u2013e10897","DOI":"10.1073\/pnas.1809548115","article-title":"Caspase-8 induces cleavage of gasdermin D to elicit pyroptosis during Yersinia infection","volume":"115","author":"Sarhan","year":"2018","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.7717\/peerj.16818\/ref-41","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.15252\/embj.201694696","article-title":"GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death","volume":"35","author":"Sborgi","year":"2016","journal-title":"EMBO Journal"},{"key":"10.7717\/peerj.16818\/ref-42","doi-asserted-by":"publisher","first-page":"112051","DOI":"10.1016\/j.jep.2019.112051","article-title":"Neuroprotective effect of glycosides in Buyang Huanwu Decoction on pyroptosis following cerebral ischemia-reperfusion injury in rats","volume":"242","author":"She","year":"2019","journal-title":"Journal of Ethnopharmacology"},{"key":"10.7717\/peerj.16818\/ref-43","doi-asserted-by":"publisher","first-page":"101233","DOI":"10.1016\/j.tranon.2021.101233","article-title":"Novel prognostic model established for patients with head and neck squamous cell carcinoma based on pyroptosis-related genes","volume":"14","author":"Shen","year":"2021","journal-title":"Translational Oncology"},{"key":"10.7717\/peerj.16818\/ref-44","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"key":"10.7717\/peerj.16818\/ref-45","doi-asserted-by":"publisher","first-page":"e52982","DOI":"10.1371\/journal.pone.0052982","article-title":"Attenuated inflammatory response in triggering receptor expressed on myeloid cells 2 (TREM2) knock-out mice following stroke","volume":"8","author":"Sieber","year":"2013","journal-title":"PLOS ONE"},{"key":"10.7717\/peerj.16818\/ref-46","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1007\/s00134-019-05705-y","article-title":"Acute ischaemic stroke: challenges for the intensivist","volume":"45","author":"Smith","year":"2019","journal-title":"Intensive Care Medicine"},{"key":"10.7717\/peerj.16818\/ref-47","doi-asserted-by":"publisher","first-page":"e124","DOI":"10.1371\/journal.pmed.0040124","article-title":"TREM2-transduced myeloid precursors mediate nervous tissue debris clearance and facilitate recovery in an animal model of multiple sclerosis","volume":"4","author":"Takahashi","year":"2007","journal-title":"PLOS Medicine"},{"key":"10.7717\/peerj.16818\/ref-48","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1084\/jem.20041611","article-title":"Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2","volume":"201","author":"Takahashi","year":"2005","journal-title":"Journal of Experimetnal Medicine"},{"key":"10.7717\/peerj.16818\/ref-49","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/s12974-014-0233-0","article-title":"NLRP1 inflammasome is activated in patients with medial temporal lobe epilepsy and contributes to neuronal pyroptosis in amygdala kindling-induced rat model","volume":"12","author":"Tan","year":"2015","journal-title":"Journal of Neuroinflammation"},{"key":"10.7717\/peerj.16818\/ref-50","first-page":"93","article-title":"[Pyroptosis and stroke]","volume":"70","author":"Tang","year":"2018","journal-title":"Sheng Li Xue Bao"},{"key":"10.7717\/peerj.16818\/ref-51","doi-asserted-by":"publisher","first-page":"3520","DOI":"10.4049\/jimmunol.177.6.3520","article-title":"Cutting edge: TREM-2 attenuates macrophage activation","volume":"177","author":"Turnbull","year":"2006","journal-title":"Journal of Immunology"},{"key":"10.7717\/peerj.16818\/ref-52","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1038\/s41467-020-15041-1","article-title":"Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin","volume":"11","author":"Wakhloo","year":"2020","journal-title":"Nature Communications"},{"key":"10.7717\/peerj.16818\/ref-53","doi-asserted-by":"publisher","first-page":"941","DOI":"10.1016\/j.cell.2020.02.002","article-title":"Structural mechanism for GSDMD targeting by autoprocessed caspases in pyroptosis","volume":"180","author":"Wang","year":"2020","journal-title":"Cell"},{"key":"10.7717\/peerj.16818\/ref-54","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s12974-019-1465-9","article-title":"Myosin1f-mediated neutrophil migration contributes to acute neuroinflammation and brain injury after stroke in mice","volume":"16","author":"Wang","year":"2019","journal-title":"Journal of Neuroinflammation"},{"key":"10.7717\/peerj.16818\/ref-55","doi-asserted-by":"publisher","first-page":"4641","DOI":"10.1016\/j.csbj.2021.07.038","article-title":"From pyroptosis, apoptosis and necroptosis to PANoptosis: a mechanistic compendium of programmed cell death pathways","volume":"19","author":"Wang","year":"2021","journal-title":"Computational and Structural Biotechnology Journal"},{"key":"10.7717\/peerj.16818\/ref-56","doi-asserted-by":"publisher","first-page":"1692","DOI":"10.1159\/000453218","article-title":"MiR-155 is involved in renal ischemia-reperfusion injury via direct targeting of FoxO3a and regulating renal tubular cell pyroptosis","volume":"40","author":"Wu","year":"2016","journal-title":"Cellular Physiology and Biochemistry"},{"key":"10.7717\/peerj.16818\/ref-57","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s13041-017-0296-9","article-title":"TREM2 protects against cerebral ischemia\/reperfusion injury","volume":"10","author":"Wu","year":"2017","journal-title":"Molecular Brain"},{"key":"10.7717\/peerj.16818\/ref-58","doi-asserted-by":"publisher","first-page":"100141","DOI":"10.1016\/j.xinn.2021.100141","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"10.7717\/peerj.16818\/ref-59","doi-asserted-by":"publisher","first-page":"F75","DOI":"10.1152\/ajprenal.00117.2013","article-title":"Ischemia-reperfusion induces renal tubule pyroptosis via the CHOP-caspase-11 pathway","volume":"306","author":"Yang","year":"2014","journal-title":"American Journal of Physiology-Renal Physiology"},{"key":"10.7717\/peerj.16818\/ref-60","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1165\/rcmb.2020-0521OC","article-title":"TREM2 Dictates antibacterial defense and viability of bone marrow-derived macrophages during bacterial infection","volume":"65","author":"Yang","year":"2021","journal-title":"American Journal of Respiratory Cell and Molecular Biology"},{"key":"10.7717\/peerj.16818\/ref-61","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS"},{"key":"10.7717\/peerj.16818\/ref-62","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.2217\/epi-2017-0151","article-title":"Exosome: a novel mediator in drug resistance of cancer cells","volume":"10","author":"Zhang","year":"2018","journal-title":"Epigenomics"},{"key":"10.7717\/peerj.16818\/ref-63","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.neulet.2016.07.036","article-title":"Neuroprotective effects of Chrysophanol against inflammation in middle cerebral artery occlusion mice","volume":"630","author":"Zhao","year":"2016","journal-title":"Neuroscience Letters"},{"key":"10.7717\/peerj.16818\/ref-64","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1038\/s41422-018-0090-y","article-title":"Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis","volume":"28","author":"Zhou","year":"2018","journal-title":"Cell Research"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/16818.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16818.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16818.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/16818.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T09:13:59Z","timestamp":1707470039000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/16818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,9]]},"references-count":64,"alternative-id":["10.7717\/peerj.16818"],"URL":"https:\/\/doi.org\/10.7717\/peerj.16818","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,9]]},"article-number":"e16818"}}