{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T08:29:40Z","timestamp":1780388980492,"version":"3.54.1"},"reference-count":45,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["82203851"],"award-info":[{"award-number":["82203851"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Sichuan Science and Technology Program","award":["2023NSFSC0731, 2023YFSY0045"],"award-info":[{"award-number":["2023NSFSC0731, 2023YFSY0045"]}]},{"name":"Nanchong Science and Technology Program","award":["22SXQT0336,20SXQT0328"],"award-info":[{"award-number":["22SXQT0336,20SXQT0328"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Junctional adhesion molecule-A (JAM-A) is an adhesion molecule that exists on the surface of certain types of cells, including white blood cells, endothelial cells, and dendritic cells. In this study, the cDNA sequences of JAM-A-Fc were chemically synthesized with optimization for mammalian expression. Afterward, we analyzed JAM-A protein expression through transient transfection in HEK293 cell lines. Mice were immunized with JAM-A-Fc protein, and hybridoma was prepared by fusing myeloma cells and mouse spleen cells. Antibodies were purified from the hybridoma supernatant and four monoclonal strains were obtained and numbered 61H9, 70E5, 71A8, and 74H3 <jats:italic>via<\/jats:italic> enzyme-linked immunosorbent assay screening. Immunofluorescence staining assay showed 61H9 was the most suitable cell line for mAb production due to its fluorescence signal being the strongest. Flow cytometric analysis proved that 61H9 possessed high affinity. Moreover, antagonism of JAM-A mAb could attenuate the proliferative, migrative, and invasive abilities of ESCC cells and significantly inhibit tumor growth in mice. By examining hematoxylin-eosin staining mice tumor tissues, we found inflammatory cells infiltrated lightly in the anti-JAM-A group. The expression of BCL-2 and I\u03baB\u03b1 in the anti-JAM-A group were decreased in mice tumor tissues compared to the control group. Ultimately, a method for preparing high-yield JAM-A-Fc protein was created and a high affinity mAb against JAM-A with an antitumor effect was prepared.<\/jats:p>","DOI":"10.7717\/peerj.17088","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:11:14Z","timestamp":1710407474000},"page":"e17088","source":"Crossref","is-referenced-by-count":1,"title":["Generation and characterization of mAb 61H9 against junctional adhesion molecule-a with potent antitumor activity"],"prefix":"10.7717","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4640-0849","authenticated-orcid":true,"given":"Kang","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China"},{"name":"Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Xiong","sequence":"additional","affiliation":[{"name":"Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunlong","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8068-4742","authenticated-orcid":true,"given":"Guiqin","family":"Song","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinliang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenling","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"issue":"8","key":"10.7717\/peerj.17088\/ref-1","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1038\/ajg.2017.155","article-title":"Predicting the future burden of esophageal cancer by histological subtype: international trends in incidence up to 2030","volume":"112","author":"Arnold","year":"2017","journal-title":"The American Journal of Gastroenterology"},{"issue":"2","key":"10.7717\/peerj.17088\/ref-2","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1007\/s10549-019-05471-x","article-title":"Functional inhibition of F11 receptor (F11R\/junctional adhesion molecule-A\/JAM-A) activity by a F11R-derived peptide in breast cancer and its microenvironment","volume":"179","author":"Bednarek","year":"2020","journal-title":"Breast Cancer Research and Treatment"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-3","doi-asserted-by":"publisher","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA: A Cancer Journal for Clinicians"},{"issue":"22","key":"10.7717\/peerj.17088\/ref-4","doi-asserted-by":"publisher","first-page":"2799","DOI":"10.1038\/onc.2012.276","article-title":"Junctional adhesion molecule-A is co-expressed with HER2 in breast tumors and acts as a novel regulator of HER2 protein degradation and signaling","volume":"32","author":"Brennan","year":"2013","journal-title":"Oncogene"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-5","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/S2468-1253(20)30007-8","article-title":"The global, regional, and national burden of oesophageal cancer and its attributable risk factors in 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017","volume":"5","author":"Collaborators","year":"2020","journal-title":"The Lancet Gastroenterology & Hepatology"},{"issue":"3","key":"10.7717\/peerj.17088\/ref-6","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1128\/JVI.80.3.1261-1270.2006","article-title":"JAM-A-independent, antibody-mediated uptake of reovirus into cells leads to apoptosis","volume":"80","author":"Danthi","year":"2006","journal-title":"Journal of Virology"},{"issue":"14","key":"10.7717\/peerj.17088\/ref-7","doi-asserted-by":"publisher","first-page":"3738","DOI":"10.1093\/emboj\/20.14.3738","article-title":"The cell polarity protein ASIP\/PAR-3 directly associates with junctional adhesion molecule (JAM)","volume":"20","author":"Ebnet","year":"2001","journal-title":"The EMBO Journal"},{"issue":"17","key":"10.7717\/peerj.17088\/ref-8","doi-asserted-by":"publisher","first-page":"2257","DOI":"10.2217\/fon-2021-0021","article-title":"Survival and complications after neoadjuvant chemotherapy or chemoradiotherapy for esophageal cancer: a meta-analysis","volume":"17","author":"Han","year":"2021","journal-title":"Future Oncology"},{"issue":"12","key":"10.7717\/peerj.17088\/ref-9","doi-asserted-by":"publisher","first-page":"1918","DOI":"10.1038\/s41417-022-00507-9","article-title":"Gastrointestinal cancer-associated fibroblasts expressing junctional adhesion molecule-a are amenable to infection by oncolytic reovirus","volume":"29","author":"Harryvan","year":"2022","journal-title":"Cancer Gene Therapy"},{"issue":"3","key":"10.7717\/peerj.17088\/ref-10","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1039\/C5AN01323B","article-title":"Diagnosis of early-stage esophageal cancer by Raman spectroscopy and chemometric techniques","volume":"141","author":"Ishigaki","year":"2016","journal-title":"The Analyst"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-11","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1016\/S1470-2045(20)30169-8","article-title":"First-line pembrolizumab and trastuzumab in HER2-positive oesophageal, gastric, or gastro-oesophageal junction cancer: an open-label, single-arm, phase 2 trial","volume":"21","author":"Janjigian","year":"2020","journal-title":"The Lancet Oncology"},{"issue":"20","key":"10.7717\/peerj.17088\/ref-12","doi-asserted-by":"publisher","first-page":"2413","DOI":"10.2217\/fon-2019-0024","article-title":"Comparison of neoadjuvant chemoradiotherapy and neoadjuvant chemotherapy for esophageal cancer: a meta-analysis","volume":"15","author":"Jing","year":"2019","journal-title":"Future Oncology"},{"issue":"8","key":"10.7717\/peerj.17088\/ref-13","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1161\/CIRCRESAHA.120.316742","article-title":"JAM-A Acts via C\/EBP-\u03b1 to promote Claudin-5 expression and enhance endothelial barrier function","volume":"127","author":"Kakogiannos","year":"2020","journal-title":"Circulation Research"},{"issue":"10111","key":"10.7717\/peerj.17088\/ref-14","doi-asserted-by":"publisher","first-page":"2461","DOI":"10.1016\/S0140-6736(17)31827-5","article-title":"Nivolumab in patients with advanced gastric or gastro-oesophageal junction cancer refractory to, or intolerant of, at least two previous chemotherapy regimens (ONO-4538-12, ATTRACTION-2): a randomised, double-blind, placebo-controlled, phase 3 trial","volume":"390","author":"Kang","year":"2017","journal-title":"The Lancet"},{"key":"10.7717\/peerj.17088\/ref-15","doi-asserted-by":"publisher","first-page":"4138","DOI":"10.1200\/jco.20.01888","article-title":"Randomized phase III KEYNOTE-181 study of pembrolizumab versus chemotherapy in advanced esophageal cancer","volume":"38","author":"Kojima","year":"2020","journal-title":"Journal of Clinical Oncology"},{"key":"10.7717\/peerj.17088\/ref-16","doi-asserted-by":"publisher","first-page":"279","DOI":"10.2147\/jpr.S33408","article-title":"NGF-the TrkA to successful pain treatment","volume":"5","author":"Kumar","year":"2012","journal-title":"Journal of Pain Research"},{"key":"10.7717\/peerj.17088\/ref-17","doi-asserted-by":"publisher","first-page":"184","DOI":"10.3978\/j.issn.2305-5839.2015.08.01","article-title":"Paradigms lost-an emerging role for over-expression of tight junction adhesion proteins in cancer pathogenesis","volume":"3","author":"Leech","year":"2015","journal-title":"Annals of Translational Medicine"},{"issue":"1","key":"10.7717\/peerj.17088\/ref-18","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1186\/s13058-018-1064-1","article-title":"Cleavage of the extracellular domain of junctional adhesion molecule-A is associated with resistance to anti-HER2 therapies in breast cancer settings","volume":"20","author":"Leech","year":"2018","journal-title":"Breast Cancer Research"},{"issue":"1","key":"10.7717\/peerj.17088\/ref-19","doi-asserted-by":"publisher","first-page":"1666","DOI":"10.1038\/s41467-023-37440-w","article-title":"Integrative proteogenomic characterization of early esophageal cancer","volume":"14","author":"Li","year":"2023","journal-title":"Nature Communications"},{"issue":"1","key":"10.7717\/peerj.17088\/ref-20","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1083\/jcb.142.1.117","article-title":"Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration","volume":"142","author":"Mart\u00ecn-Padura","year":"1998","journal-title":"The Journal of Cell Biology"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-21","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1002\/ijc.24498","article-title":"JAM-A expression positively correlates with poor prognosis in breast cancer patients","volume":"125","author":"McSherry","year":"2009","journal-title":"International Journal of Cancer"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-22","doi-asserted-by":"publisher","first-page":"e21242","DOI":"10.1371\/journal.pone.0021242","article-title":"Abrogation of junctional adhesion molecule-a expression induces cell apoptosis and reduces breast cancer progression","volume":"6","author":"Murakami","year":"2011","journal-title":"PLOS ONE"},{"issue":"3","key":"10.7717\/peerj.17088\/ref-23","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1242\/jcs.114.3.539","article-title":"Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody","volume":"114","author":"Naik","year":"2001","journal-title":"Journal of Cell Science"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-24","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1007\/s10147-020-01652-7","article-title":"Comparison of involved field radiotherapy and elective nodal irradiation in combination with concurrent chemotherapy for T1bN0M0 esophageal cancer","volume":"25","author":"Nakatani","year":"2020","journal-title":"International Journal of Clinical Oncology"},{"issue":"4","key":"10.7717\/peerj.17088\/ref-25","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1161\/01.ATV.0000157154.14474.3b","article-title":"Involvement of JAM-A in mononuclear cell recruitment on inflamed or atherosclerotic endothelium: inhibition by soluble JAM-A","volume":"25","author":"Ostermann","year":"2005","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"issue":"2","key":"10.7717\/peerj.17088\/ref-26","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1053\/j.gastro.2021.10.017","article-title":"Burden and cost of gastrointestinal, liver, and pancreatic diseases in the United States: update 2021","volume":"162","author":"Peery","year":"2022","journal-title":"Gastroenterology"},{"issue":"12","key":"10.7717\/peerj.17088\/ref-27","doi-asserted-by":"publisher","first-page":"e538","DOI":"10.1016\/S1470-2045(14)70442-5","article-title":"International myeloma working group updated criteria for the diagnosis of multiple myeloma","volume":"15","author":"Rajkumar","year":"2014","journal-title":"Lancet Oncology"},{"issue":"18","key":"10.7717\/peerj.17088\/ref-28","doi-asserted-by":"publisher","first-page":"4691","DOI":"10.1182\/blood-2010-10-299487","article-title":"Consensus recommendations for the uniform reporting of clinical trials: report of the International Myeloma Workshop Consensus Panel 1","volume":"117","author":"Rajkumar","year":"2011","journal-title":"Blood"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-29","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.3390\/cancers13061286","article-title":"Development of a novel weighted ranking method for immunohistochemical quantification of a heterogeneously expressed protein in gastro-esophageal cancers","volume":"13","author":"Richards","year":"2021","journal-title":"Cancers"},{"issue":"2","key":"10.7717\/peerj.17088\/ref-30","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1080\/14728222.2022.2036718","article-title":"Esophageal cancer: emerging therapeutics","volume":"26","author":"Rogers","year":"2022","journal-title":"Expert Opinion on Therapeutic Targets"},{"issue":"11","key":"10.7717\/peerj.17088\/ref-31","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1634\/theoncologist.2018-0143","article-title":"Comparative molecular analyses of esophageal squamous cell carcinoma, esophageal adenocarcinoma, and gastric adenocarcinoma","volume":"23","author":"Salem","year":"2018","journal-title":"The Oncologist"},{"issue":"11","key":"10.7717\/peerj.17088\/ref-32","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1038\/labinvest.3700671","article-title":"Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture","volume":"87","author":"Schellerer","year":"2007","journal-title":"Laboratory Investigation"},{"issue":"12","key":"10.7717\/peerj.17088\/ref-33","doi-asserted-by":"publisher","first-page":"3614","DOI":"10.3390\/cancers12123614","article-title":"Long-Term outcomes of induction chemotherapy followed by chemo-radiotherapy as intensive neoadjuvant protocol in patients with esophageal cancer","volume":"12","author":"Simoni","year":"2020","journal-title":"Cancers"},{"issue":"5","key":"10.7717\/peerj.17088\/ref-34","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1016\/j.annonc.2021.02.004","article-title":"Checkpoint inhibitors for gastroesophageal cancers: dissecting heterogeneity to better understand their role in first-line and adjuvant therapy","volume":"32","author":"Smyth","year":"2021","journal-title":"Annals of Oncology"},{"issue":"3","key":"10.7717\/peerj.17088\/ref-35","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/leu.2017.287","article-title":"JAM-A as a prognostic factor and new therapeutic target in multiple myeloma","volume":"32","author":"Solimando","year":"2018","journal-title":"Leukemia"},{"issue":"7","key":"10.7717\/peerj.17088\/ref-36","doi-asserted-by":"publisher","first-page":"1943","DOI":"10.3324\/haematol.2019.239913","article-title":"Halting the vicious cycle within the multiple myeloma ecosystem: blocking JAM-A on bone marrow endothelial cells restores angiogenic homeostasis and suppresses tumor progression","volume":"106","author":"Solimando","year":"2021","journal-title":"Haematologica"},{"issue":"8","key":"10.7717\/peerj.17088\/ref-37","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1007\/s00018-017-2729-0","article-title":"Junctional adhesion molecule-a: functional diversity through molecular promiscuity","volume":"75","author":"Steinbacher","year":"2018","journal-title":"Cellular and Molecular Life Sciences"},{"issue":"21","key":"10.7717\/peerj.17088\/ref-38","doi-asserted-by":"publisher","first-page":"4117","DOI":"10.2174\/0929867327666201111142307","article-title":"A critical transcription factor NF-\u03baB as a cancer therapeutic target and its inhibitors as cancer treatment options","volume":"28","author":"Suhail","year":"2021","journal-title":"Current Medicinal Chemistry"},{"issue":"9","key":"10.7717\/peerj.17088\/ref-39","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1038\/nri.2017.52","article-title":"The non-canonical NF-\u03baB pathway in immunity and inflammation","volume":"17","author":"Sun","year":"2017","journal-title":"Nature Reviews Immunology"},{"issue":"6","key":"10.7717\/peerj.17088\/ref-40","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1038\/s41575-021-00419-3","article-title":"Global burden and epidemiology of Barrett oesophagus and oesophageal cancer","volume":"18","author":"Thrift","year":"2021","journal-title":"Nature Reviews Gastroenterology & Hepatology"},{"issue":"1","key":"10.7717\/peerj.17088\/ref-41","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1093\/carcin\/bgu230","article-title":"Junctional adhesion molecule-A, an epithelial-mesenchymal transition inducer, correlates with metastasis and poor prognosis in human nasopharyngeal cancer","volume":"36","author":"Tian","year":"2015","journal-title":"Carcinogenesis"},{"issue":"1","key":"10.7717\/peerj.17088\/ref-42","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1007\/s13577-022-00806-1","article-title":"HOXD11 upregulates JAM-A and exerts oncogenic properties via NF-\u03baB signaling pathway in esophageal squamous cell carcinoma","volume":"36","author":"Xiong","year":"2023","journal-title":"Human Cell"},{"issue":"11","key":"10.7717\/peerj.17088\/ref-43","doi-asserted-by":"publisher","first-page":"e79173","DOI":"10.1371\/journal.pone.0079173","article-title":"Overexpression of JAM-A in non-small cell lung cancer correlates with tumor progression","volume":"8","author":"Zhang","year":"2013","journal-title":"PLOS ONE"},{"issue":"4","key":"10.7717\/peerj.17088\/ref-44","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1007\/s00432-021-03659-7","article-title":"Neoadjuvant chemoradiotherapy versus neoadjuvant chemotherapy for the treatment of esophageal squamous cell carcinoma: a propensity score-matched study from the National Cancer Center in China","volume":"148","author":"Zhang","year":"2022","journal-title":"Journal of Cancer Research and Clinical Oncology"},{"issue":"2","key":"10.7717\/peerj.17088\/ref-45","doi-asserted-by":"publisher","first-page":"1010428317691000","DOI":"10.1177\/1010428317691000","article-title":"Overexpression of junctional adhesion molecule-A and EphB2 predicts poor survival in lung adenocarcinoma patients","volume":"39","author":"Zhao","year":"2017","journal-title":"Tumour Biology"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/17088.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/17088.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/17088.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/17088.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:11:26Z","timestamp":1710407486000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/17088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,14]]},"references-count":45,"alternative-id":["10.7717\/peerj.17088"],"URL":"https:\/\/doi.org\/10.7717\/peerj.17088","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,14]]},"article-number":"e17088"}}