{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T04:53:20Z","timestamp":1769316800837,"version":"3.49.0"},"reference-count":26,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific research project of Changzhou Medical Center of Nanjing Medical University","award":["CZKYCMCB2022251"],"award-info":[{"award-number":["CZKYCMCB2022251"]}]},{"name":"Science and Technology Project of Changzhou","award":["CJ20220097, CE2022507"],"award-info":[{"award-number":["CJ20220097, CE2022507"]}]},{"name":"Project of Changzhou Health Commission","award":["QN202129, ZD202120"],"award-info":[{"award-number":["QN202129, ZD202120"]}]},{"name":"Young Talent Development Plan of Changzhou Health Commission","award":["CZQM2022017, CZQM2020091"],"award-info":[{"award-number":["CZQM2022017, CZQM2020091"]}]},{"name":"Jiangsu Province Traditional Chinese Medicine Technology Development Project","award":["MS2022078"],"award-info":[{"award-number":["MS2022078"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Single nucleotide polymorphisms (SNPs), as the most abundant form of DNA variation in the human genome, contribute to age-related cataracts (ARC) development. Apoptosis of lens epithelial cells (LECs) is closely related to ARC formation. Insulin-like growth factor 1 (IGF1) contributes to cell apoptosis regulation. Moreover, IGF1 was indicated to exhibit a close association with cataract formation. Afterward, an investigation was conducted to examine the correlation between polymorphisms in <jats:italic>IGF1<\/jats:italic> and the susceptibility to ARC.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>The present investigation was a case-control study. Venous blood draws were collected from the participants for DNA genotyping. Lens capsule samples were collected to detect mRNA and apoptosis. TaqMan RT-PCR was used to detect <jats:italic>IGF1<\/jats:italic> polymorphism genotypes and qRT PCR was used to detect <jats:italic>IGF1<\/jats:italic> mRNA levels in LECs. LEC apoptosis was evaluated through flow cytometry. The chi-square test was used to compare differences between ARCs and controls of each SNP.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>We found that the G allele frequency in the <jats:italic>IGF1<\/jats:italic>-rs6218 was higher in the ARCs than in the controls. Furthermore, it was observed that the rs6218 GG genotype exhibited a positive correlation to elevated levels of <jats:italic>IGF1<\/jats:italic> mRNA in LECs. The <jats:italic>IGF1<\/jats:italic> mRNA in the LECs and the apoptosis of LECs in nuclear type of ARCs (ARNC) was higher than the controls.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The susceptibility to ARC was related to <jats:italic>IGF1<\/jats:italic>-rs6218 polymorphism, and this polymorphism is associated with <jats:italic>IGF1<\/jats:italic> expression at the mRNA level. Moreover, apoptosis in LECs of ARNCs was found to be increased.<\/jats:p>\n<\/jats:sec>","DOI":"10.7717\/peerj.17220","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T07:37:26Z","timestamp":1712648246000},"page":"e17220","source":"Crossref","is-referenced-by-count":1,"title":["<i>IGF-1<\/i> rs6218 polymorphisms modulate the susceptibility to age-related cataract"],"prefix":"10.7717","volume":"12","author":[{"given":"Xi","family":"Zou","sequence":"first","affiliation":[{"name":"Changzhou Medical Center, Changzhou, China"},{"name":"The Third People\u2019s Hospital of Changzhou, Changzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Third People\u2019s Hospital of Changzhou, Changzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Nantong First People\u2019s 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