{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:32:44Z","timestamp":1716337964308},"reference-count":33,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Project of Changzhou Health Commission","award":["QN202317"],"award-info":[{"award-number":["QN202317"]}]},{"name":"Scientific Research Project of Changzhou Medical Center of Nanjing Medical University","award":["CMCP202303 to HL"],"award-info":[{"award-number":["CMCP202303 to HL"]}]},{"name":"Jiangsu Funding Program for Excellent Postdoctoral Talent","award":["2023ZB319 to HL"],"award-info":[{"award-number":["2023ZB319 to HL"]}]},{"name":"Changzhou Sci&Tech Program","award":["CJ20230073"],"award-info":[{"award-number":["CJ20230073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Circular RNAs (circRNAs) are a large class of RNAs present in mammals. Among these, <jats:italic>circCamsap1<\/jats:italic> is a well-acknowledged circRNA with significant implications, particularly in the development and progression of diverse tumors. However, the potential consequences of <jats:italic>circCamsap1<\/jats:italic> depletion <jats:italic>in vivo<\/jats:italic> on male reproduction are yet to be thoroughly investigated.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>The presence of <jats:italic>circCamsap1<\/jats:italic> in the mouse testes was confirmed, and gene expression analysis was performed using reverse transcription quantitative polymerase chain reaction. <jats:italic>CircCamsap1<\/jats:italic> knockout mice were generated utilizing the CRISPR\/Cas9 system. Phenotypic analysis of both the testes and epididymis was conducted using histological and immunofluorescence staining. Additionally, fertility and sperm motility were assessed.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Here, we successfully established a <jats:italic>circCamsap1<\/jats:italic> knockout mouse model without affecting the expression of parental gene. Surprisingly, male mice lacking <jats:italic>circCamsap1<\/jats:italic> (<jats:italic>circCamsap1<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>) exhibited normal fertility, with no discernible differences in testicular and epididymal histology, spermatogenesis, sperm counts or sperm motility compared to <jats:italic>circCamsap1<jats:sup>+\/+<\/jats:sup><\/jats:italic> mice. These findings suggest that <jats:italic>circCamsap1<\/jats:italic> may not play an essential role in physiological spermatogenesis. Nonetheless, this result also underscores the complexity of circRNA function in male reproductive biology. Therefore, further research is necessary to elucidate the precise roles of other circRNAs in regulating male fertility.<\/jats:p>\n<\/jats:sec>","DOI":"10.7717\/peerj.17399","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T07:25:43Z","timestamp":1716276343000},"page":"e17399","source":"Crossref","is-referenced-by-count":0,"title":["CircCamsap1 is dispensable for male fertility in mice"],"prefix":"10.7717","volume":"12","author":[{"given":"Shu","family":"Zhang","sequence":"first","affiliation":[{"name":"Center of Reproduction, The Affiliated Changzhou Second People\u2019s Hospital of Nanjing Medical University, Changzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haojie","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Reproduction, The Affiliated Changzhou Second People\u2019s Hospital of Nanjing Medical University, Changzhou, Jiangsu, China"},{"name":"Changzhou Medical Center, The 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