{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:26:27Z","timestamp":1721694387216},"reference-count":44,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Hunan Forestry Science and Technology Innovation Fund Project","award":["XLKY202221"],"award-info":[{"award-number":["XLKY202221"]}]},{"name":"The National Key R&D Program of China","award":["2017YFD0601304"],"award-info":[{"award-number":["2017YFD0601304"]}]},{"DOI":"10.13039\/501100004761","name":"The Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2022JJ30998"],"award-info":[{"award-number":["2022JJ30998"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p><jats:italic> Colletotrichum fructicola<\/jats:italic> is a predominant anthracnose species in <jats:italic>Camellia oleifera<\/jats:italic>, causing various adverse effects. Traditional intercropping <jats:italic>Vernicia fordii<\/jats:italic> with <jats:italic>C. oleifera<\/jats:italic> may enhance anthracnose resistance, but the mechanism remains elusive.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>We utilized UPLC-MS\/MS and acid-base detection to identify the major antimicrobial alkaloid components in the <jats:italic>V. fordii<\/jats:italic> leaf extract. Subsequently, by adding different concentrations of <jats:italic>V. fordii<\/jats:italic> leaf extract for cultivating <jats:italic>C. fructicola<\/jats:italic>, with untreated <jats:italic>C. fructicola<\/jats:italic> as a control, we investigated the impact of the <jats:italic>V. fordii<\/jats:italic> leaf extract, cell wall integrity, cell membrane permeability, MDA, and ROS content changes. Additionally, analysis of key pathogenic genes of <jats:italic>C. fructicola<\/jats:italic> confirmed that the <jats:italic>V. fordii<\/jats:italic> leaf extract inhibits the growth of the fungus through gene regulation.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>This study discovered the alkaloid composition of <jats:italic>V. fordii<\/jats:italic> leaf extract by UPLC-MS\/MS and acid-base detection, such as trigonelline, stachydrine, betaine, and O-Phosphocholine. <jats:italic>V. fordii<\/jats:italic> leaf extract successfully penetrated <jats:italic>C. fructicola<\/jats:italic> mycelia, damaged cellular integrity, and increased ROS and MDA levels by 1.75 and 2.05 times respectively, thereby inhibiting <jats:italic>C. fructicola<\/jats:italic> proliferation. By analyzing the key pathogenic genes of <jats:italic>C. fructicola<\/jats:italic>, it was demonstrated that the antifungal function of <jats:italic>V. fordii<\/jats:italic> leaf extract depends mainly on the regulation of <jats:italic>RAB7<\/jats:italic> and <jats:italic>HAC1<\/jats:italic> gene expression. Therefore, this study elucidates the mechanism of <jats:italic>V. fordii<\/jats:italic> -<jats:italic>C. oleifera<\/jats:italic> intercropping in strengthening anthracnose resistance in<jats:italic> C. oleifera<\/jats:italic>, contributing to efficient <jats:italic>C. oleifera<\/jats:italic> cultivation.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.17607","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T07:56:46Z","timestamp":1721635006000},"page":"e17607","source":"Crossref","is-referenced-by-count":0,"title":["<i>Vernicia fordii<\/i> leaf extract inhibited anthracnose growth by downregulating reactive oxygen species (ROS) levels <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.7717","volume":"12","author":[{"given":"Luyao","family":"Ge","sequence":"first","affiliation":[{"name":"Central South University of Forestry and Technology, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, 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