{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:15:19Z","timestamp":1792602919543,"version":"4.5.0"},"reference-count":83,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais\u2013FAPEMIG","award":["# APQ-00361-16"],"award-info":[{"award-number":["# APQ-00361-16"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2013CNPq","award":["# 404365\/2016-7"],"award-info":[{"award-number":["# 404365\/2016-7"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2013CNPq","award":["#304334\/2019-7"],"award-info":[{"award-number":["#304334\/2019-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>The integration of diagnostic methods holds promise for advancing the surveillance of malaria transmission in both endemic and non-endemic regions. Serological assays emerge as valuable tools to identify and delimit malaria transmission, serving as a complementary method to rapid diagnostic tests (RDT) and thick smear microscopy. Here, we evaluate the potential of antibodies directed against peptides encompassing the entire amino acid sequence of the <jats:italic>Pv<\/jats:italic>MSP-1 Sal-I strain as viable serological biomarkers for <jats:italic>P. vivax<\/jats:italic> exposure.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>We screened peptides encompassing the complete amino acid sequence of the <jats:italic>Plasmodium vivax<\/jats:italic> Merozoite Surface Protein 1 (<jats:italic>Pv<\/jats:italic>MSP-1) Sal-I strain as potential biomarkers for <jats:italic>P. vivax<\/jats:italic> exposure. Here, immunodominant peptides specifically recognized by antibodies from individuals infected with <jats:italic>P. vivax<\/jats:italic> were identified using the SPOT-synthesis technique followed by immunoblotting. Two 15-mer peptides were selected based on their higher and specific reactivity in immunoblotting assays. Subsequently, peptides p70 and p314 were synthesized in soluble form using SPPS (Solid Phase Peptide Synthesis) and tested by ELISA (IgG, and subclasses).<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>This study unveils the presence of IgG antibodies against the peptide p314 in most <jats:italic>P. vivax<\/jats:italic>-infected individuals from the Brazilian Amazon region. <jats:italic>In silico<\/jats:italic> B-cell epitope prediction further supports the utilization of p314 as a potential biomarker for evaluating malaria transmission, strengthened by its amino acid sequence being part of a conserved block of <jats:italic>Pv<\/jats:italic>MSP-1. Indeed, compared to patients infected with <jats:italic>P. falciparum<\/jats:italic> and uninfected individuals never exposed to malaria, <jats:italic>P. vivax<\/jats:italic>-infected patients have a notably higher recognition of p314 by IgG1 and IgG3.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.17632","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T07:33:35Z","timestamp":1719300815000},"page":"e17632","source":"Crossref","is-referenced-by-count":3,"title":["Identification and serological responses to a novel <i>Plasmodium vivax<\/i> merozoite surface protein 1 (<i>Pv<\/i>MSP-1) derived synthetic peptide: a putative biomarker for malaria exposure"],"prefix":"10.7717","volume":"12","author":[{"given":"Aline","family":"Marzano-Miranda","sequence":"first","affiliation":[{"name":"Department of Parasitology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 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