{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:44:32Z","timestamp":1791290672132,"version":"4.3.0"},"reference-count":34,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wuhu Science and Technology Project","award":["No. 2021yf67"],"award-info":[{"award-number":["No. 2021yf67"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Acute pancreatitis (AP) is a sudden-onset disease of the digestive system caused by abnormal activation of pancreatic enzymes. Dual oxidase 2 (DUOX2) has been found to be elevated in the progression of a variety of inflammatory diseases. Therefore, we analyzed the specific roles of DUOX2 in AP development. Blood samples were collected from of AP patients and healthy people, and the caerulein- stimulated human pancreatic duct cells (H6C7) were utilized to establish an AP cell model. Cell growth and apoptosis were measured using an MTT assay and TUNEL staining. Additionally, RT-qPCR and western blot assays were conducted to assess the RNA and protein expressions of the cells. ELISA kits were used to determine TNF-\u03b1, IL-6, IL-8, and IL-1\u03b2 levels. The interaction between DUOX2 and miR-605-3p was predicted using the Targetscan database and confirmed by dual-luciferase report assay. We found that DUOX2 increased while miR-605-3p decreased in the blood of AP patients and caerulein-stimulated H6C7 cells. DUOX2 was targeted by miR-605-3p. Furthermore, DUOX2 knockdown or miR-605-3p overexpression promoted cell viability, decreased the TNF-\u03b1, IL-6, IL-8, and IL-1\u03b2 levels, and inhibited apoptosis rate in caerulein-stimulated H6C7 cells. DUOX2 knockdown or miR-605-3p overexpression also increased the Bcl-2 protein levels and down-regulated Bax, cleaved-caspase-1, NLRP3 and p-p65. Interestingly, DUOX2 overexpression reversed the miR-605-3p mimic function in the caerulein-treated H6C7 cells. In conclusion, our research demonstrated that DUOX2 knockdown relieved the injury and inflammation in caerulein-stimulated H6C7 cells.<\/jats:p>","DOI":"10.7717\/peerj.17874","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T07:44:47Z","timestamp":1725003887000},"page":"e17874","source":"Crossref","is-referenced-by-count":5,"title":["miR-605-3p may affect caerulein-induced ductal cell injury and pyroptosis in acute pancreatitis by targeting the DUOX2\/NLRP3\/NF-\u03baB pathway"],"prefix":"10.7717","volume":"12","author":[{"given":"Gai","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Emergency Internal Medicine, The First Affiliated Hospital of Wannan Medical College Yijishan Hospital, Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncology, First Affiliated Hospital, Gannan Medical University, Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Emergency Surgery, The First Affiliated Hospital of Wannan Medical College Yijishan Hospital, Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Emergency Internal Medicine, The First Affiliated Hospital of Wannan Medical College Yijishan Hospital, Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghui","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Emergency Internal Medicine, The First Affiliated Hospital of Wannan Medical College Yijishan Hospital, Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenli","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Oncology, First 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