{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T03:21:12Z","timestamp":1793503272478,"version":"4.5.0"},"reference-count":36,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Medical Science and Technology Research Fund Project of Guangdong Province","award":["A2019547, B2024099"],"award-info":[{"award-number":["A2019547, B2024099"]}]},{"name":"Science and Technology Project of Zengcheng District, Guangzhou","award":["ZCKJ2019-003"],"award-info":[{"award-number":["ZCKJ2019-003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Colonic epithelial cell apoptosis and pyroptosis had a close relationship with the pathological progression of ulcerative colitis (UC). LncRNA play a crucial role in the progression of UC. However, the role of the lncRNA MALAT1 in colonic epithelial cell apoptosis and pyroptosis remains unclear.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>UC colitis cell model was established through lipopolysaccharide (LPS) treatment. MiR-22-3p and MALAT1 expression in fetal human colon (FHC) cells were analyzed by qRT-PCR. Proliferation and apoptosis of FHCs were measured using CCK-8 assay and flow cytometry, respectively. Pyroptosis indicators including interleukin (IL)-1\u03b2, IL-18, tumor necrosis factor-\u03b1 (TNF-\u03b1), NLR family pyrin domain containing 3 (NLRP3), caspase-1, and N-gasdermin D (N-GSDMD) in FHCs were detected using ELISA, qRT-PCR, western blotting, and immunofluorescence.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>In this study, apoptosis was facilitated, IL-1\u03b2, IL-18, and TNF-\u03b1 levels were enhanced, NLRP3, caspase-1, N-GSDMD protein were increased, and MALAT1 expression was markedly increased in LPS-treated FHCs (LTFs). MALAT1 knockdown remarkably facilitated proliferation and suppressed apoptosis, reduced IL-1\u03b2, IL-18, and TNF-\u03b1 levels, and decreased the protein of NLRP3, caspase-1, N-GSDMD. Furthermore, NLRP3 overexpression remarkably reversed the effect of MALAT1-downexpression in LTFs. In addition, miR-22-3p could bind with MALAT1 and NLRP3 3\u2032 UTR. Furthermore, miR-22-3p inhibition remarkably reversed the effect of MALAT1 overexpression in LTFs.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>These findings suggest that MALAT1 represents a promising therapeutic target for the treatment of UC by modulating the miR-22-3p\/NLRP3 pathway, potentially leading to novel strategies for reducing inflammation and cell death in the colon.<\/jats:p>\n<\/jats:sec>","DOI":"10.7717\/peerj.18449","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T07:24:54Z","timestamp":1731914694000},"page":"e18449","source":"Crossref","is-referenced-by-count":8,"title":["MALAT1 promotes colonic epithelial cell apoptosis and pyroptosis by sponging miR-22-3p to enhance NLRP3 expression"],"prefix":"10.7717","volume":"12","author":[{"given":"Rong","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghua","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"issue":"7","key":"10.7717\/peerj.18449\/ref-1","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1093\/ibd\/izy383","article-title":"Systematic review and meta-analysis: optimal salvage therapy in acute severe ulcerative colitis","volume":"25","author":"Choy","year":"2019","journal-title":"Inflammatory Bowel Diseases"},{"issue":"6","key":"10.7717\/peerj.18449\/ref-2","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1080\/03008207.2018.1439480","article-title":"Knockdown of LncRNA MALAT1 contributes to the suppression of inflammatory responses by up-regulating miR-146a in LPS-induced acute lung injury","volume":"59","author":"Dai","year":"2018","journal-title":"Connective Tissue Research"},{"key":"10.7717\/peerj.18449\/ref-3","doi-asserted-by":"publisher","first-page":"97","DOI":"10.3389\/fncel.2020.00097","article-title":"Blocking the LncRNA MALAT1\/miR-224-5p\/NLRP3 Axis Inhibits the Hippocampal Inflammatory Response in T2DM With OSA","volume":"14","author":"Du","year":"2020","journal-title":"Frontiers in Cellular Neuroscience"},{"issue":"1","key":"10.7717\/peerj.18449\/ref-4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5114\/pg.2023.125882","article-title":"Guidelines for the management of ulcerative colitis. 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