{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T05:39:39Z","timestamp":1738215579259,"version":"3.34.0"},"reference-count":66,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Key Research and Development Project of Hebei Province","award":["20277746D and 22377744D"],"award-info":[{"award-number":["20277746D and 22377744D"]}]},{"name":"Shijiazhuang Science and Technology Research and Development Self-financing Plan","award":["211200443"],"award-info":[{"award-number":["211200443"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The immune status of Coronavirus disease 2019 (COVID-19) patients in different stages of infection remains difficult to determine. In this study, we performed high-throughput single-cell mass cytometry on peripheral blood samples from 10 COVID-19 patients and four healthy donors to analyze their immune status at acute and convalescence phases. During the acute stage, the proportion of neutrophils increased significantly while natural killer (NK) cells decreased. In contrast, during the convalescence phase, the proportion of plasma cells decreased from the acute stage of disease onset and was lower than normal. The proportions of B, mast and plasma cell subsets decreased significantly with the process of disease recovery. Further analysis of the subsets of major immune cell types in COVID-19 patients with different clinical presentations in different stages showed that in the acute stages of disease progression, the T helper cell 1 (Th1), IgD<jats:sup>+<\/jats:sup> B and neutrophil subsets increased in COVID-19 patients, especially in symptomatic patients, while the central memory CD4<jats:sup>+<\/jats:sup>T cells (CD4 TCM), mucosa-associated invariant T (MAIT) and NK cell subsets decreased significantly, especially in symptomatic patients. Then CD4 TCM and MAIT returned to normal levels at the recovery phase. Dynamic assessment displayed that the immune imbalance at the onset of COVID-19 could be corrected during recovery. Our study provides additional information on the immune status of COVID-19 patients with different clinical manifestations in different stages. These findings may provide new insights into COVID-19 immunotherapy and immune intervention.<\/jats:p>","DOI":"10.7717\/peerj.18451","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T08:00:46Z","timestamp":1738137646000},"page":"e18451","source":"Crossref","is-referenced-by-count":0,"title":["Different immunological characteristics of asymptomatic and symptomatic COVID-19 patients without vaccination in the acute and convalescence stages"],"prefix":"10.7717","volume":"13","author":[{"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, China"},{"name":"Intensive Care Unit, The Fifth Hospital of Shijiazhuang, Shijiazhuang, Hebei, China"},{"name":"Hebei Key Laboratory of Immune Mechanism of Major Infectious Diseases and New Technology of Diagnosis and Treatment, The Fifth Hospital of Shijiazhuang, Shijiazhuang, Hebei, 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