{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:56:30Z","timestamp":1790596590859,"version":"4.1.0"},"reference-count":59,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>The application of plant extracts and their phytochemicals as potential treatments for bacterial illnesses has increased significantly in the last few decades. In Ethiopia, <jats:italic>Echinops kebericho<\/jats:italic> Mesfin is widely used to treat a range of illnesses in humans and animals. This study aimed to evaluate the antibacterial activity and phytochemical screening of <jats:italic>Echinops kebericho<\/jats:italic> Mesfin.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>We carried out an <jats:italic>in vitro<\/jats:italic> experimental study after collecting the plants from their natural habitats. Then macerated in absolute methanol and petroleum ether solvents and concentrated the extracts using a rotary evaporator. In the experiment, we used Standard cultures of <jats:italic>E. coli<\/jats:italic>, <jats:italic>K. pneumoniae<\/jats:italic>, <jats:italic>S. aureus<\/jats:italic>, and <jats:italic>P. aeruginosa<\/jats:italic>. The agar-well diffusion method evaluated the antibacterial activity of the plants. The agar dilution method determined the minimum inhibitory concentration of the plant extract.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>The percentage yield of the plant extracts ranged from 6.25% to 7.85%. The methanol extract of <jats:italic>Echinops kebericho<\/jats:italic> Mesfin had the highest inhibitory effect on <jats:italic>S.\u00a0aureus<\/jats:italic> (ATCC 25923) (16.67 \u00b1 0.58 mm), followed by <jats:italic>E. coli<\/jats:italic> (ATCC 25922) (11.0\u00a0\u00b1 1.73 mm). Phytochemical screening of leaves from the methanol and petroleum ether extracts of the plant revealed the presence of phytochemicals such as alkaloids, flavonoids, tannins, and cardiac glycosides. The present study revealed that the extracts of these plants have antibacterial activity. However, researchers should conduct further studies on the safety margin and quantitative bioactive isolation of selected medicinal plants.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.18554","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T13:12:55Z","timestamp":1733231575000},"page":"e18554","source":"Crossref","is-referenced-by-count":4,"title":["Phytochemical screening and <i>in vitro<\/i> antibacterial activity of <i>Echinops kebericho<\/i> Mesfin tuber extracts: experimental studies"],"prefix":"10.7717","volume":"12","author":[{"given":"Jiregna Gari","family":"Negasa","sequence":"first","affiliation":[{"name":"Department of Veterinary Laboratory Technology, School of Veterinary Medicine, Ambo University,  Ambo, Oromia, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibsa","family":"Teshome","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Ambo University, Ambo, Oromia, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edilu Jorga","family":"Sarba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Ambo University, Ambo, Oromia, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekiyad Shasho","family":"Daro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Epidemiology, College of Veterinary Medicine, Haramaya University,  Dire dawa, Oromia, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"issue":"1","key":"10.7717\/peerj.18554\/ref-1","doi-asserted-by":"publisher","first-page":"15593258211004739","DOI":"10.1177\/15593258211004739","article-title":"Phytochemical analysis, antioxidant and antimicrobial screening of Seriphidium oliverianum plant extracts","volume":"19","author":"Abbas","year":"2021","journal-title":"Dose-Response"},{"issue":"1","key":"10.7717\/peerj.18554\/ref-2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4103\/jpbs.jpbs_175_19","article-title":"Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes","volume":"12","author":"Abubakar","year":"2020","journal-title":"Journal of Pharmacy and Bioallied Sciences"},{"key":"10.7717\/peerj.18554\/ref-3","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.2147\/idr.s398204","article-title":"In vitro antibacterial activities of selected medicinal plants used by traditional healers for treating urinary tract infection in haramaya district, Eastern Ethiopia","volume":"16","author":"Ahmed","year":"2023","journal-title":"Infection and Drug Resistance"},{"issue":"2","key":"10.7717\/peerj.18554\/ref-4","doi-asserted-by":"publisher","first-page":"403","DOI":"10.18596\/jotcsa.836074","article-title":"Phytochemical screening and antibacterial activity of pistacia atlantica and pinus canariensis extracts","volume":"8","author":"Alhajali","year":"2021","journal-title":"Journal of the Turkish Chemical Society Section A: Chemistry"},{"issue":"2","key":"10.7717\/peerj.18554\/ref-5","first-page":"15","article-title":"Chemical constituents of Datura stramonium L. 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