{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:57:45Z","timestamp":1788670665001,"version":"build-2803163510"},"reference-count":48,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Urbino Carlo Bo","award":["446 23.10.2020"],"award-info":[{"award-number":["446 23.10.2020"]}]},{"name":"European Union\u2014Next Generation EU\u2014PNRR MUR","award":["ECS_00000041-VITALITY\u2014CUP J13C22000430001"],"award-info":[{"award-number":["ECS_00000041-VITALITY\u2014CUP J13C22000430001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Forty-eight million people worldwide suffer from dementia, often associated with the growth of the elderly population. There are also concerns about the younger population, where increasing acute and chronic abuse of alcohol and neurotoxic substances may contribute to brain damage and the early onset of dementia. Alzheimer\u2019s disease (AD) accounts for 60% of dementia cases and most therapies used so far have been unsuccessful. Genetic, epigenetic and vascular factors contribute to the pathogenesis of AD. Among the epigenetic mechanisms, modulation of microRNA (miRs) plays an important role. To detect genes and pathways involved in AD, we performed an original bioinformatic analysis of published Alzheimer\u2019s dysregulated miRs using MIcroRNA ENrichment TURned NETwork (MIENTURNET) followed by Reactome tools. The interrogation of these platforms allowed us to discover common putative genes (by MIENTURNET) targeted by the dysregulated miRs and the pathways in which the set of altered genes are involved (by Reactome tool). Our <jats:italic>in silico<\/jats:italic> analysis showed that the \u03b2-catenin phosphorylation cascade and Netrin-1 signalling, resulted as the most significant. Lastly, based on the assumption that food bioactive compounds (BC) modulate miRs, which in turn modulate dysregulated genes and pathways associated with AD, a literature search demonstrated that some BC are indeed able to modulate dysregulated pathways and genes. Curcumin, osthole, puerarin, xanthoceraside, sulforaphane, salvianolic acid A, resveratrol and andrographolide lead to upregulation of the Wnt\/\u03b2-catenin pathway. Choline, methionine, folate and vitamin B6\/B12 modulate the upregulation of the Netrin-1 pathway. In conclusion, our <jats:italic>in silico<\/jats:italic> analysis of miRs identified dysregulated genes and their associated pathways, paving interesting and new insights for diagnosis and for potential therapeutic interventions.<\/jats:p>","DOI":"10.7717\/peerj.19100","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T08:06:20Z","timestamp":1744013180000},"page":"e19100","source":"Crossref","is-referenced-by-count":1,"title":["<i>In silico<\/i> detection of dysregulated genes and molecular pathways in Alzheimer\u2019s disease as basis for food restoring approach"],"prefix":"10.7717","volume":"13","author":[{"given":"Ilaria","family":"Petrignani","sequence":"first","affiliation":[{"name":"Diagnostics and Metrology Laboratory (NUC-TECFIS-DIM), Research Center ENEA, Frascati, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Pasquo","sequence":"additional","affiliation":[{"name":"Diagnostics and Metrology 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