{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:43:54Z","timestamp":1783529034508,"version":"3.55.0"},"reference-count":44,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Project of People\u2019s Hospital of Suzhou New District","award":["SGY2022C01, SGY2023C02"],"award-info":[{"award-number":["SGY2022C01, SGY2023C02"]}]},{"name":"Project of Suzhou","award":["GSWS2023036"],"award-info":[{"award-number":["GSWS2023036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Circular ribonucleic acid (RNA) protein kinase C iota (circ-PRKCI, hsa_circRNA_0122683) has been previously reported to be involved in the development of sepsis. However, the knowledge regarding the potential role and mechanism of circ-PRKCI in sepsis-induced acute lung injury (ALI) is unclear. An <jats:italic>in vitro<\/jats:italic> cellular model of sepsis-ALI was simulated by the treatment of lipopolysaccharide (LPS) in human pulmonary alveolar epithelial cells (HPAEpiCs). The expression of circ-PRKCI in plasma samples from sepsis patients with or without ALI as well as sepsis-ALI cell model was determined by quantitative real-time PCR (qRT-PCR). The diagnostic utility of circ-PRKCI was analyzed using receiver operating characteristic (ROC) curves. The levels of iron content (Fe<jats:sup>2+<\/jats:sup>), glutathione (GSH), malondialdehyde (MDA), and reactive oxygen species (ROS) were detected using corresponding commercial kits. The assessment of cell viability and production of pro-infammatory cytokines (IL-6, IL-1\u03b2 and TNF-\u03b1) was measured using Cell Counting Kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA). The targeting relationship between circ-PRKCI and miR-382-5p was predicted by bioinformatics analysis, and subsequently confirmed by luciferase reporter and RNA immunoprecipitation (RIP) assays. Results shows that decreased circ-PRKCI expression but increased miR-382-5p expression was observed in sepsis patients with ALI and sepsis-induced ALI cell model. The area under the curve values of ROC curves for circ-PRKCI in differentiating septic ALI patients from healthy individuals and septic non-ALI patients were 0.996 and 0.999, respectively. Functional <jats:italic>in vitro<\/jats:italic> assays revealed that enforced expression of circ-PRKCI alleviated LPS-induced ferroptosis and inflammatory response of HPAEpiCs, which were reversed by Erastin or FIN56 administration. Mechanistically, circ-PRKCI was identified as a sponge of miR-382-5p and negatively regulated miR-382-5p expression. Further rescue experiments showed that miR-382-5p overexpression could compromise the anti-ferroptosis and anti-inflammatory response effects of circ-PRKCI on LPS-induced injury of HPAEpiCs. Our study demonstrated that circ-PRKCI may be a promising biomarker for septic ALI diagnosis. circ-PRKCI inhibits ferroptosis and inflammatory response in sepsis-induced ALI by sponging miR-382-5p, indicating that the circ-PRKCI\/miR-382-5p axis might be a novel therapeutic target for the treatment of sepsis-induced ALI.<\/jats:p>","DOI":"10.7717\/peerj.19404","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T08:16:56Z","timestamp":1747124216000},"page":"e19404","source":"Crossref","is-referenced-by-count":4,"title":["Has_circRNA_0122683 (circ-PRKCI) relieves ferroptosis of HPAEpiCs in sepsis-induced acute lung injury by sponging miR-382-5p"],"prefix":"10.7717","volume":"13","author":[{"given":"Limei","family":"Yan","sequence":"first","affiliation":[{"name":"Intensive Care Medicine Department, The People\u2019s Hospital of Suzhou New District, Suzhou, 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