{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T16:04:43Z","timestamp":1782403483642,"version":"3.54.5"},"reference-count":30,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Natural Science Foundation of Yunnan Province","award":["202001BA070001-133"],"award-info":[{"award-number":["202001BA070001-133"]}]},{"DOI":"10.13039\/501100013047","name":"Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province","doi-asserted-by":"crossref","award":["D-2017057"],"award-info":[{"award-number":["D-2017057"]}],"id":[{"id":"10.13039\/501100013047","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Yunnan Natural Science Foundation of the Department of Education","award":["2020J0590"],"award-info":[{"award-number":["2020J0590"]}]},{"name":"Science and Technology Project of Dali City","award":["2021KBG083"],"award-info":[{"award-number":["2021KBG083"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>Ischemic stroke (IS) is a major health concern in the Chinese population. Previous studies have highlighted the role of NRF2 in IS. This study investigates the association between NRF2 polymorphisms and IS susceptibility in a Chinese population.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>This retrospective study included Chinese patients diagnosed with IS based on clinical symptoms, neurological examinations, and brain imaging findings from computed tomography or magnetic resonance imaging. Age- and sex-matched unrelated individuals with no family history of stroke, tumors, or genetic diseases served as controls. Peripheral blood samples were collected to genotype seven single-nucleotide polymorphisms (SNPs) in NRF2 (rs13005431, rs4893819, rs6721961, rs35652124, rs6726395, rs2364723, rs2706110) using the SNaPshot method. Binary logistic regression analysis was used to assess associations between these SNPs and IS risk. NRF2 and reactive oxygen species (ROS) levels in peripheral blood were measured. The relationship between rs35652124 and NRF2 expression was evaluated using expression quantitative trait locus (eQTL) analysis.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>All seven NRF2 SNPs conformed to Hardy\u2013Weinberg equilibrium. Six SNPs (rs13005431, rs4893819, rs6721961, rs6726395, rs2364723, and rs2706110) showed no significant differences in distribution between the case and control groups (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). However, the TC genotype of rs35652124 in the co-dominant model was significantly associated with increased IS risk. The distribution of this genotype aligned with trends observed in East Asia and the Chinese Han population but varied across other global populations. The CCTTGGC haplotype was the most common in both groups. Stratified analysis of rs35652124 showed no association with confounding factors such as age, sex, hypertension, diabetes, or lipid levels. NRF2 and ROS levels were higher in IS patients than in controls, but did not differ by rs35652124 genotype. Concurrently, eQTL analysis indicated that rs35652124 did not affect NRF2 expression in peripheral blood.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusion<\/jats:title>\n               <jats:p>The NRF2 rs35652124 polymorphism is associated with IS susceptibility, suggesting it may be a potential genetic risk factor for IS.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.19742","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T08:27:19Z","timestamp":1753345639000},"page":"e19742","source":"Crossref","is-referenced-by-count":2,"title":["<i>NRF2<\/i> polymorphism and susceptibility to ischemic stroke in a Chinese population"],"prefix":"10.7717","volume":"13","author":[{"given":"Pengyu","family":"Wang","sequence":"first","affiliation":[{"name":"School of Clinical Medicine, Dali University, Dali, Yunnan Province, China"},{"name":"Clinical Laboratory, Baoding No.1 Central Hospital, Baoding, Hebei Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxiu","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Dali University, Dali, Yunnan Province, China"},{"name":"The People\u2019s Hospital of Junan, Linyi, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Dali University, Dali, Yunnan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Dali University, Dali, Yunnan Province, China"},{"name":"Center of Genetic Testing, The First Affiliated Hospital of Dali University, Dali, Yunnan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaqiu","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Xichang People\u2019s Hospital, Xichang, Sichuan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"10.7717\/peerj.19742\/ref-1","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.ijcard.2016.11.049","article-title":"The adverse impact of obesity on heart rate variability is modified by a NFE2L2 gene variant: the SAPALDIA cohort","volume":"228","author":"Adam","year":"2017","journal-title":"International Journal of Cardiology"},{"key":"10.7717\/peerj.19742\/ref-2","doi-asserted-by":"publisher","first-page":"e10218","DOI":"10.1016\/j.heliyon.2022.e10218","article-title":"Molecular mechanisms underlying some major common risk factors of 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