{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:03:13Z","timestamp":1756425793432,"version":"3.44.0"},"reference-count":25,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hangzhou Biomedical and Health Industry Development Support Science and Technology","award":["2022WJC284"],"award-info":[{"award-number":["2022WJC284"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n               <jats:title>Background<\/jats:title>\n               <jats:p>A large number of new human immunodeficiency virus (HIV) patients are diagnosed worldwide every year. This study aimed to design a rapid, efficient, and convenient kit for detecting HIV-1 viral load.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Methods<\/jats:title>\n               <jats:p>The sensitivity and specificity of the kit were determined, and serum samples were collected from HIV-negative and HIV-positive individuals who underwent medical checkups at Xixi Hospital in Hangzhou City, Zhejiang Province, China, between June and August 2023 for testing. The presence of HIV-1 in the clinical samples was assessed <jats:italic>via<\/jats:italic> a diagnostic PCR system and a conventional fluorescent quantitative PCR technique in this study.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Results<\/jats:title>\n               <jats:p>HIV-1 RNA was measured with a 100 copies\/mL sensitivity <jats:italic>via<\/jats:italic> the MultNAT HIV-1 Viral Load Assay. The accuracy of the a ssay was as follows: The assay showed near-perfect agreement with compare kit (kappa = 0.9945), positive compliance rate of 99.45% (182\/183), negative compliance rate of 100% (183\/183). The linear correlation coefficient between MultNAT HIV-1 Viral Load Assay and DaAn kit was 0.95, and the R2 was 0.90.<\/jats:p>\n            <\/jats:sec>\n            <jats:sec>\n               <jats:title>Conclusion<\/jats:title>\n               <jats:p>Due to its simplicity, rapid response time, and high sensitivity and specificity, the MultNAT HIV-1 Viral Load Assay is suitable for primary care and epidemiological screening, facilitating rapid and accurate HIV-1 detection.<\/jats:p>\n            <\/jats:sec>","DOI":"10.7717\/peerj.19753","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T08:06:02Z","timestamp":1756368362000},"page":"e19753","source":"Crossref","is-referenced-by-count":0,"title":["MultNAT HIV-1 Viral Load Assay: a simple, rapid and sensitive cross-primer amplification assay for detecting the HIV-1 virus in the 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