{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T19:12:39Z","timestamp":1769713959984,"version":"3.49.0"},"reference-count":43,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Northwest Potato Research Consortium"},{"DOI":"10.13039\/100012125","name":"Agricultural Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012125","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Oregon State University"},{"name":"USDA New Beginning"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Thiamin is essential for human health, but humans do not synthesize it and must consume it through regular dietary intake. Plants synthesize thiamin in photosynthetic tissues to support various primary metabolic pathways. In addition, plants can also absorb thiamin from the soil. Interestingly, plant growth can be improved by supplying exogenous thiamin, but this effect has not been investigated in potato (\n                    <jats:italic>Solanum tuberosum<\/jats:italic>\n                    ). Here, we report the effect of soil thiamin amendment on yield and tissue thiamin content of three potato varieties grown in a greenhouse. We watered plants with different concentrations of thiamin on a weekly basis from emergence until harvest. Under optimal growth conditions, thiamin supplementation did not affect tuber yield, regardless of soil type. Thiamin accumulated up to 58, six and three times in roots, tubers and stems, respectively, of plants grown in soil supplemented with thiamin compared to control plants, while leaf thiamin content did not significantly change. Quantitative polymerase chain reaction analysis showed that soil thiamin supplementation had no or little effect on the expression of two key thiamin biosynthesis genes in leaves. Our results indicate that increasing soil thiamin concentration does not improve potato yield under optimal growth conditions at the greenhouse scale. However, increased yield at field-scale under stress conditions remains to be tested. Intriguingly, the accumulation of thiamin in potato tubers suggests that soil thiamin supplementation may be a viable strategy for biofortification.\n                  <\/jats:p>","DOI":"10.7717\/peerj.20684","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T08:36:27Z","timestamp":1769675787000},"page":"e20684","source":"Crossref","is-referenced-by-count":0,"title":["Thiamin addition to soil increases potato tuber thiamin content under greenhouse conditions"],"prefix":"10.7717","volume":"14","author":[{"given":"Aymeric","family":"Goyer","sequence":"first","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi","family":"Phillips","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aidan","family":"Seidel","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Handy","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Schiffer","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra J.","family":"Weisberg","sequence":"additional","affiliation":[{"name":"Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul C.","family":"Bethke","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture\u2014Agricultural Research Service, Madison, WI,  United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"10.7717\/peerj.20684\/ref-1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1023\/A:1010255526903","article-title":"Counteraction of salinity stress on wheat plants by grain soaking in ascorbic acid, thiamin or sodium salicylate","volume":"44","author":"Al-Hakimi","year":"2001","journal-title":"Biologia 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of vitamin B1 to the growth of green plants","volume":"101","author":"Bonner","year":"1939","journal-title":"Botanical Gazette"},{"key":"10.7717\/peerj.20684\/ref-6","doi-asserted-by":"publisher","DOI":"10.1101\/084715","article-title":"Pavian: interactive analysis of metagenomics data for microbiomics and pathogen identification","author":"Breitwieser","year":"2016","journal-title":"BioRxiv"},{"key":"10.7717\/peerj.20684\/ref-7","doi-asserted-by":"publisher","first-page":"3129","DOI":"10.3390\/plants12173129","article-title":"Potato non-specific lipid transfer protein StnsLTPI.33 is associated with the production of reactive oxygen species, plant growth, and susceptibility to Alternaria solani","volume":"12","author":"Bvindi","year":"2023","journal-title":"Plants"},{"key":"10.7717\/peerj.20684\/ref-8","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1038\/nature10503","article-title":"Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase","volume":"478","author":"Chatterjee","year":"2011","journal-title":"Nature"},{"issue":"3","key":"10.7717\/peerj.20684\/ref-9","first-page":"22","article-title":"The new Automated Mass Spectrometry Deconvolution and Identification System (AMDIS)","volume":"10","author":"Davies","year":"1998","journal-title":"Spectroscopy Europe"},{"key":"10.7717\/peerj.20684\/ref-10","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/BF02712670","article-title":"A plant DNA minipreparation: version II","volume":"1","author":"Dellaporta","year":"1983","journal-title":"Plant Molecular Biology Reporter"},{"key":"10.7717\/peerj.20684\/ref-11","doi-asserted-by":"publisher","first-page":"2285","DOI":"10.1093\/pcp\/pcv148","article-title":"Enhancement of thiamin content in Arabidopsis thaliana by metabolic engineering","volume":"56","author":"Dong","year":"2015","journal-title":"Plant and Cell 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heritability","volume":"88","author":"Goyer","year":"2011","journal-title":"American Journal of Potato Research"},{"key":"10.7717\/peerj.20684\/ref-16","doi-asserted-by":"publisher","first-page":"13072","DOI":"10.1021\/jf203736e","article-title":"Genetic diversity of thiamin and folate in primitive cultivated and wild potato (Solanum) species","volume":"59","author":"Goyer","year":"2011","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"10.7717\/peerj.20684\/ref-17","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.plantsci.2018.01.019","article-title":"Redesigning thiamin synthesis: prospects and potential payoffs","volume":"273","author":"Hanson","year":"2018","journal-title":"Plant Science"},{"key":"10.7717\/peerj.20684\/ref-18","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.1104\/pp.16.01371","article-title":"Does abiotic stress cause functional B vitamin deficiency in 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GitHub","author":"Vill","year":"2023"},{"key":"10.7717\/peerj.20684\/ref-41","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/BF02486227","article-title":"Beitr\u00e4ge zur Kenntnis des Vitamingehaltes von Belebtschlamm aus Abwasserreinigungsanlagen","volume":"14","author":"Von Kocher","year":"1952","journal-title":"Schweizerische Zeitschrift f\u00fcr Hydrologie"},{"issue":"1\u20139","key":"10.7717\/peerj.20684\/ref-42","doi-asserted-by":"publisher","first-page":"10489","DOI":"10.4161\/rna.7.1.10489","article-title":"Riboswitch-mediated control of gene expression in eukaryotes","volume":"7","author":"Wachter","year":"2010","journal-title":"RNA Biology"},{"key":"10.7717\/peerj.20684\/ref-43","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1186\/s13059-019-1891-0","article-title":"Improved metagenomic analysis with Kraken 2","volume":"20","author":"Wood","year":"2019","journal-title":"Genome Biology"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/20684.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/20684.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/20684.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/20684.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T08:36:35Z","timestamp":1769675795000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/20684"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,29]]},"references-count":43,"alternative-id":["10.7717\/peerj.20684"],"URL":"https:\/\/doi.org\/10.7717\/peerj.20684","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,29]]},"article-number":"e20684"}}