{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:42:43Z","timestamp":1770748963215,"version":"3.50.0"},"reference-count":35,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Projects of Top Talent Support Program for young and middle-aged people of Wuxi Health Committee","award":["No. HB2023090"],"award-info":[{"award-number":["No. HB2023090"]}]},{"DOI":"10.13039\/501100008109","name":"Wuxi Municipal Bureau on Science and Technology","doi-asserted-by":"crossref","award":["No. Y20212042"],"award-info":[{"award-number":["No. Y20212042"]}],"id":[{"id":"10.13039\/501100008109","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Top Talent Support Program for young and middle-aged people of Wuxi Health Committee","award":["No. BJ2023095"],"award-info":[{"award-number":["No. BJ2023095"]}]},{"name":"Wuxi Medical Development Disciplines","award":["No. FZXK2021010"],"award-info":[{"award-number":["No. FZXK2021010"]}]},{"name":"COVID-19 Emergency Project of Jiangsu Provincial Science and Technology Department","award":["No. BE2023603"],"award-info":[{"award-number":["No. BE2023603"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    The COVID-19 pandemic has substantially altered respiratory pathogen circulation, underscoring the critical need for pre-pandemic baseline data to interpret current epidemiological trends. To establish this baseline, we employed metatranscriptomic sequencing to characterize the etiology of community-acquired pneumonia (CAP) in 20 adult patients hospitalized in Wuxi, China, during 2018\u20132019. Following ribosomal RNA depletion, sequencing data were analyzed using a stringent dual-filter strategy (RPM\u00a0\u2265\u00a0100 and Z-score\u00a0\u2265\u00a02) to identify high-confidence pathogens. Our analysis revealed a complex, polymicrobial landscape. Bacterial pathogens predominated, with\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    species detected in 25% of cases. The frequent co-occurrence of oral anaerobes (\n                    <jats:italic>e.g.<\/jats:italic>\n                    ,\n                    <jats:italic>Prevotella<\/jats:italic>\n                    ,\n                    <jats:italic>Veillonella<\/jats:italic>\n                    ,\n                    <jats:italic>Rothia<\/jats:italic>\n                    ) suggested that aspiration-driven polymicrobial infections were a key pathogenic mechanism. Viral pathogens were also prominent, with\n                    <jats:italic>Orthorubulavirus hominis<\/jats:italic>\n                    and Human respirovirus 1 showing significant transcriptional activity. Notably, our approach enabled the discovery and characterization of two divergent viral strains: a novel Rhinovirus B strain (AP81) with only 90.52% nucleotide identity to its closest relative, and a picobirnavirus phylogenetically distinct from human strains (94.90% identity to a simian-derived virus). Fungal detection was minimal, with only\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    meeting the criteria in a single case. In conclusion, this study provides a crucial pre-pandemic baseline of CAP etiology in Wuxi. It highlights the power of metatranscriptomics to not only define common etiological agents but also to uncover novel viral diversity and reveal the polymicrobial complexity of respiratory infections, offering critical insights for future surveillance and clinical management.\n                  <\/jats:p>","DOI":"10.7717\/peerj.20774","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T08:38:12Z","timestamp":1770712692000},"page":"e20774","source":"Crossref","is-referenced-by-count":0,"title":["A retrospective metatranscriptomic study of respiratory pathogens causing adult community-acquired pneumonia in Wuxi, China, before the pandemic"],"prefix":"10.7717","volume":"14","author":[{"given":"Yan-Jun","family":"Kang","sequence":"first","affiliation":[{"name":"Affiliated Children\u2019s Hospital of Jiangnan University, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumeng","family":"Gao","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujun","family":"Chen","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Shi","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"},{"name":"Nanjing Medical University, Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Shen","sequence":"additional","affiliation":[{"name":"Wuxi Center for Disease Control and Prevention, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"10.7717\/peerj.20774\/ref-1","doi-asserted-by":"publisher","first-page":"S248","DOI":"10.1093\/clinids\/16.Supplement_4.S248","article-title":"Anaerobic bacterial infections of the lung and pleural space","volume":"16","author":"Bartlett","year":"1993","journal-title":"Clinical Infectious Diseases"},{"key":"10.7717\/peerj.20774\/ref-2","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1038\/s41592-021-01101-x","article-title":"Sensitive protein alignments at tree-of-life scale using DIAMOND","volume":"18","author":"Buchfink","year":"2021","journal-title":"Nature 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