{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:04:00Z","timestamp":1789376640619,"version":"build-2803163510"},"reference-count":22,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Projects in Guangzhou","award":["SL2024A03J01056"],"award-info":[{"award-number":["SL2024A03J01056"]}]},{"name":"Science and Technology Projects in Guangzhou","award":["202201020234"],"award-info":[{"award-number":["202201020234"]}]},{"DOI":"10.13039\/100007452","name":"Wu Jieping Medical Foundation","doi-asserted-by":"crossref","award":["320.6750.2024-06-7"],"award-info":[{"award-number":["320.6750.2024-06-7"]}],"id":[{"id":"10.13039\/100007452","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82371650"],"award-info":[{"award-number":["82371650"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2024A1515030071"],"award-info":[{"award-number":["2024A1515030071"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Fund for Lin He Academician New Medicine","award":["2021HLKY06"],"award-info":[{"award-number":["2021HLKY06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    To date, no fully automated liquid-phase embryo tank has been implemented in assisted reproductive technology (ART) laboratory and its impact on the safety and efficacy of embryo storage remains unknown. To evaluate its performance, the Hatchferti group (HG) stored a total of 230 clinically discarded 3PN zygotes (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0143) and day 3 cleavage embryos (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a087) from September 2023 to April 2024. These embryos were vitrified and stored for 30, 90, and 180 days, their survival and culture results were statistically analyzed. The total 3PN zygote cleavage rate after thawing was compared with the fresh 2PN zygote cleavage rate of 2023 laboratory annual data (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a032,520). A 7-day period of workload data showed that the operating time in conventional liquid nitrogen tank group (CG) required for storage and retrieval were 6.71\u00a0\u00b1\u00a01.28 and 39.00\u00a0\u00b1\u00a013.42 minutes, respectively, while that in HG were significantly lower (1.95\u00a0\u00b1\u00a00.04 and 0.96\u00a0\u00b1\u00a00.03 minutes respectively,\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). In order to assess the stability of the operating temperature, during the 6-month study period, the temperature readings in the retrieval area were \u2212177.84\u00a0\u00b1\u00a01.53\u00a0\u00b0C, and the storage area temperatures were \u2212195.47\u00a0\u00b1\u00a02.01\u00a0\u00b0C and \u2212195.81\u00a0\u00b1\u00a00.94\u00a0\u00b0C meeting the expected temperature for embryo storage. In the comparison between different freezing time groups, there was no statistically significant difference in the recovery rates of 3PN embryos and day 3 cleavage embryos between groups (both 100%,\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05). There was no statistically significant difference in the cleavage rate of 3PN embryos after thawing between groups (95.56%, 95.83%, 100%,\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05, respectively). There was no significant difference in the total zygote cleavage rate after thawing between HG (for the entire experimental period) and 2023 laboratory annual data (97.2%, 97.76%,\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05). In conclusion, Hatchferti has demonstrated its safety and efficacy, showing promising potential for application in the ART laboratory.\n                  <\/jats:p>","DOI":"10.7717\/peerj.21703","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:25:28Z","timestamp":1789374328000},"page":"e21703","source":"Crossref","is-referenced-by-count":0,"title":["Safety and efficacy of the automated liquid-phase embryo storage, Hatchferti: a pilot study"],"prefix":"10.7717","volume":"14","author":[{"given":"Yaming","family":"Meng","sequence":"first","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Bai","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangrong","family":"Gao","sequence":"additional","affiliation":[{"name":"Guangzhou Pinjie Biological Tech Co., Ltd, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxian","family":"Jiang","sequence":"additional","affiliation":[{"name":"Guangzhou PINZHI Medical Device Co., Ltd, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueliang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zeng","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Mao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangzhou PINZHI Medical Device Co., Ltd, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Reproductive Medicine of Guangdong Province, Guangzhou Medical University,  Guangzhou, China"},{"name":"Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"10.7717\/peerj.21703\/ref-1","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1007\/s43032-022-00941-y","article-title":"Automation in ART: paving the way for the future of infertility treatment","volume":"30","author":"Abdullah","year":"2023","journal-title":"Reproductive Sciences"},{"issue":"5","key":"10.7717\/peerj.21703\/ref-2","doi-asserted-by":"publisher","first-page":"104348","DOI":"10.1016\/j.rbmo.2024.104348","article-title":"Effort in manual operation of reproductive health cryostorage facilities","volume":"49","author":"Broussard","year":"2024","journal-title":"Reproductive Biomedicine Online"},{"key":"10.7717\/peerj.21703\/ref-3","doi-asserted-by":"publisher","first-page":"103252","DOI":"10.1016\/j.rbmo.2023.06.007","article-title":"Cryostorage management of reproductive cells and tissues in ART: status, needs, opportunities and potential new challenges","volume":"47","author":"Canosa","year":"2023","journal-title":"Reproductive Biomedicine Online"},{"key":"10.7717\/peerj.21703\/ref-4","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.xfnr.2021.08.001","article-title":"Are we approaching automated assisted reproductive technology? Embryo culture, metabolomics, and cryopreservation","volume":"2","author":"Casciani","year":"2021","journal-title":"F & S Reviews"},{"key":"10.7717\/peerj.21703\/ref-5","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.xfre.2020.05.010","article-title":"Embryo cryopreservation and utilization in the United States from 2004\u20132013","volume":"1","author":"Christianson","year":"2020","journal-title":"F & S Reports"},{"key":"10.7717\/peerj.21703\/ref-6","doi-asserted-by":"publisher","first-page":"103237","DOI":"10.1016\/j.rbmo.2023.05.009","article-title":"First babies conceived with automated intracytoplasmic sperm injection","volume":"47","author":"Costa-Borges","year":"2023","journal-title":"Reproductive Biomedicine Online"},{"key":"10.7717\/peerj.21703\/ref-7","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1016\/j.rbmo.2017.06.015","article-title":"The Vienna consensus: report of an expert meeting on the development of ART laboratory performance indicators","volume":"35","author":"ESHRE Special Interest Group of Embryology and Alpha Scientists in Reproductive Medicine","year":"2017","journal-title":"Reproductive BioMedicine Online"},{"key":"10.7717\/peerj.21703\/ref-8","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.xfre.2021.04.006","article-title":"Comparison of electronic versus manual witnessing of procedures within the in vitro fertilization laboratory: impact on timing and efficiency","volume":"2","author":"Holmes","year":"2021","journal-title":"F & S Reports"},{"key":"10.7717\/peerj.21703\/ref-9","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1095\/biolreprod.109.077685","article-title":"Equilibrium vitrification of mouse embryos","volume":"82","author":"Jin","year":"2010","journal-title":"Biology of Reproduction"},{"key":"10.7717\/peerj.21703\/ref-10","doi-asserted-by":"publisher","first-page":"695","DOI":"10.13283\/j.cnki.xdfckjz.2017.09.009","article-title":"Application of a novel tubular frozen carrier in the management of human embryo banks","volume":"26","author":"Li","year":"2017","journal-title":"Tongfang Knowledge Network (Beijing) Technology Co., Ltd"},{"key":"10.7717\/peerj.21703\/ref-11","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/s42003-021-01937-1","article-title":"Development of deep learning algorithms for predicting blastocyst formation and quality by time-lapse monitoring","volume":"4","author":"Liao","year":"2021","journal-title":"Communications Biology"},{"issue":"3","key":"10.7717\/peerj.21703\/ref-12","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1016\/j.xfss.2021.06.005","article-title":"Evaluation of the TMRW vapor phase cryostorage platform using reproductive specimens and in vitro extended human embryo culture","volume":"2","author":"Logsdon","year":"2021","journal-title":"F & S Science"},{"key":"10.7717\/peerj.21703\/ref-13","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/0011-2240(86)90049-0","article-title":"Devitrification in glass-forming aqueous solutions","volume":"23","author":"Macfarlane","year":"1986","journal-title":"Cryobiology"},{"key":"10.7717\/peerj.21703\/ref-14","doi-asserted-by":"publisher","first-page":"e267-e274","DOI":"10.1097\/PTS.0000000000000763","article-title":"Catastrophic human error in assisted reproductive technologies: a systematic review","volume":"18","author":"Nesbit","year":"2022","journal-title":"Journal of Patient Safety"},{"key":"10.7717\/peerj.21703\/ref-15","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.fertnstert.2022.01.018","article-title":"The ART of cryopreservation and its changing landscape","volume":"117","author":"Pomeroy","year":"2022","journal-title":"Fertility and Sterility"},{"key":"10.7717\/peerj.21703\/ref-16","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1016\/j.rbmo.2022.06.028","article-title":"Developing a novel device. Eggcell, to improve temperature stability during oocyte collection for IVF","volume":"45","author":"Prathalingam","year":"2022","journal-title":"Reproductive Biomedicine Online"},{"key":"10.7717\/peerj.21703\/ref-17","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.theriogenology.2014.04.003","article-title":"Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos","volume":"82","author":"Sansinena","year":"2014","journal-title":"Theriogenology"},{"key":"10.7717\/peerj.21703\/ref-18","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1080\/14647270701553280","article-title":"Risks associated with cryopreservation: a survey of assisted conception units in the UK and Ireland","volume":"11","author":"Tomlinson","year":"2008","journal-title":"Human Fertility"},{"key":"10.7717\/peerj.21703\/ref-19","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1039\/D0LC00874E","article-title":"FertDish: microfluidic sperm selection-in-a-dish for intracytoplasmic sperm injection","volume":"21","author":"Xiao","year":"2021","journal-title":"Lab on a Chip"},{"key":"10.7717\/peerj.21703\/ref-20","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1186\/s12884-023-05879-w","article-title":"Effect of exposed-to-air frequency of cryopreserved embryo on clinical outcomes of vitrified-warmed embryo transfer cycles: a retrospective analysis of 9,200 vitrified-warmed transfer cycles","volume":"23","author":"Zhang","year":"2023","journal-title":"BMC Pregnancy and Childbirth"},{"key":"10.7717\/peerj.21703\/ref-21","doi-asserted-by":"publisher","first-page":"16490","DOI":"10.1038\/s41598-023-43665-y","article-title":"Evaluation of an automated dish preparation system for IVF and embryo culture using a mouse mode","volume":"13","author":"Zhu","year":"2023","journal-title":"Scientific Reports"},{"key":"10.7717\/peerj.21703\/ref-22","doi-asserted-by":"publisher","first-page":"1330684","DOI":"10.3389\/fcell.2023.1330684","article-title":"Automation in vitrification and thawing of mouse oocytes and embryos","volume":"11","author":"Zhu","year":"2023","journal-title":"Frontiers in Cell and Developmental Biology"}],"container-title":["PeerJ"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/peerj.com\/articles\/21703.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/21703.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/21703.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/peerj.com\/articles\/21703.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:25:31Z","timestamp":1789374331000},"score":1,"resource":{"primary":{"URL":"https:\/\/peerj.com\/articles\/21703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,14]]},"references-count":22,"alternative-id":["10.7717\/peerj.21703"],"URL":"https:\/\/doi.org\/10.7717\/peerj.21703","archive":["CLOCKSS","LOCKSS","Portico"],"relation":{},"ISSN":["2167-8359"],"issn-type":[{"value":"2167-8359","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,14]]},"article-number":"e21703"}}