{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:58:32Z","timestamp":1790585912847,"version":"4.1.0"},"reference-count":64,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Capacity Enhancement Program for Mid-Career and Young Faculty in Guangxi Universities","award":["2025KY0445"],"award-info":[{"award-number":["2025KY0445"]}]},{"name":"Guangxi Natural Science Foundation","award":["2023GXNSFAA026440"],"award-info":[{"award-number":["2023GXNSFAA026440"]}]},{"name":"Opening Foundation of Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University","award":["NNNU-KLOP-X2006"],"award-info":[{"award-number":["NNNU-KLOP-X2006"]}],"id":[{"id":"https:\/\/ror.org\/04dx82x73","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Basic Scientific Research Fund of the Guangxi Academy of Agricultural Sciences, China","award":["2026YT004"],"award-info":[{"award-number":["2026YT004"]}],"id":[{"id":"https:\/\/ror.org\/020rkr389","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Continuous cassava cropping can lead to soil degradation and disturbances in soil microbial functions, threatening the sustainability of cassava production systems. To address these challenges, this study examined the effects of biochar on soil physicochemical properties and microbial functional profiles in a continuous cassava cropping system. Two biochar application rates (0 and 3 Mg ha\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) were applied, and metagenomic sequencing was conducted to evaluate microbial community composition and functional genes related to carbon and nitrogen cycling in rhizosphere and bulk soils. The results demonstrated that biochar was associated with higher soil pH, soil organic matter, and available nutrient content, with a stronger effect in rhizosphere soil. Biochar application altered the genetic potential of microbial communities, particularly in the rhizosphere soil. In terms of functional categories, biochar was related to higher gene abundances in \u201chomologous recombination\u201d and \u201cDNA replication\u201d categories (Kyoto Encyclopedia of Genes and Genomes (KEGG) database), as well as the \u201creplication, recombination, and repair\u201d category (evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) database) in rhizosphere soil. Biochar also affected the abundance of carbon cycling genes, particularly in the rhizosphere. The abundance of the aerobic respiration-related gene\n                    <jats:italic>coxA<\/jats:italic>\n                    was increased, while the abundance of the anaerobic fermentation gene L-lactate dehydrogenase (\n                    <jats:italic>LDH<\/jats:italic>\n                    ) was decreased. Additionally, in rhizosphere soil, biochar significantly increased the abundance of\n                    <jats:italic>norB<\/jats:italic>\n                    (denitrification),\n                    <jats:italic>GDH2<\/jats:italic>\n                    (nitrogen mineralization), and\n                    <jats:italic>nifD<\/jats:italic>\n                    (nitrogen fixation), while decreasing the abundance of genes involved in nitrogen assimilation (\n                    <jats:italic>gltB<\/jats:italic>\n                    ), assimilatory nitrate reduction (\n                    <jats:italic>nirA<\/jats:italic>\n                    ), nitrogen mineralization (\n                    <jats:italic>cynS<\/jats:italic>\n                    ), and nitrogen uptake (\n                    <jats:italic>nrtA, nasF, cynA, nrtC, and nasD<\/jats:italic>\n                    ). Together, these results suggest that biochar application may enhance nutrient availability and reshape microbial functional potential primarily in the cassava rhizosphere, providing field evidence for biochar use in continuous cassava cropping.\n                  <\/jats:p>","DOI":"10.7717\/peerj.21731","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:30:21Z","timestamp":1790584221000},"page":"e21731","source":"Crossref","is-referenced-by-count":0,"title":["Biochar application alters soil properties and microbial gene profiles in a continuous cassava cropping system"],"prefix":"10.7717","volume":"14","author":[{"given":"Yanmei","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning, China"},{"name":"Guangxi Geographical Indication Crops Research Center of Big Data Mining and Experimental Engineering Technology, Nanning Normal University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning, China"},{"name":"Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yundong","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangxi Subtropical Crops Research Institute, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangxi Subtropical Crops Research Institute, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingming","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning, China"},{"name":"Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4443","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"10.7717\/peerj.21731\/ref-1","doi-asserted-by":"publisher","first-page":"141454","DOI":"10.1016\/j.jclepro.2024.141454","article-title":"Identifying biochar production variables to maximise exchangeable cations and increase nutrient availability in soils","volume":"446","author":"Adhikari","year":"2024","journal-title":"Journal of Cleaner Production"},{"key":"10.7717\/peerj.21731\/ref-2","doi-asserted-by":"publisher","first-page":"102433","DOI":"10.1016\/j.rineng.2024.102433","article-title":"Biochar for a sustainable future: environmentally friendly production and diverse 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