{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:21:48Z","timestamp":1792790508195,"version":"4.5.0"},"reference-count":47,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2022A1515012602"],"award-info":[{"award-number":["2022A1515012602"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2023A1515140133"],"award-info":[{"award-number":["2023A1515140133"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2022A1515140025"],"award-info":[{"award-number":["2022A1515140025"]}]},{"name":"Department of Education of Guangdong Province Characteristic Innovative Talents Project of Ordinary University","award":["2023KTSCX146"],"award-info":[{"award-number":["2023KTSCX146"]}],"id":[{"id":"https:\/\/ror.org\/01s5gyw91","id-type":"ROR","asserted-by":"crossref"}]},{"name":"2022 Huizhou Science and Technology Plan Project in the Medical and Health Field","award":["No. 2022CZ010200"],"award-info":[{"award-number":["No. 2022CZ010200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The main component of potatoes is starch, which can serve as an easy breeding ground for\n                      <jats:italic>Bacillus cereus<\/jats:italic>\n                      during storage. This can decrease the shelf life of potatoes and pose food safety issues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study isolated several strains of\n                      <jats:italic>B. cereus<\/jats:italic>\n                      from rotten potatoes in the market as well as one of their bacteriophages, Bacillus phage PHZU0001, from municipal sewage. The genome of Bacillus phage PHZU0001 was sequenced. The phage sensitivity to eight\n                      <jats:italic>B. cereus<\/jats:italic>\n                      strains was detected using plaque assay. Stability assays assessed the phage\u2019s thermal and pH tolerance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that this bacteriophage had wide host range against\n                      <jats:italic>B. cereus<\/jats:italic>\n                      of different strains. Based on its genome sequence, this bacteriophage is from the\n                      <jats:italic>Herelleviridae<\/jats:italic>\n                      family. The biological characteristics of this bacteriophage indicated that it maintains stability at temperatures ranging from 23\u00a0\u00b0C to 50\u00a0\u00b0C and at pH levels ranging from 5 to 9. Inoculation of Bacillus phage PHZU0001 in potatoes effectively inhibited infection by\n                      <jats:italic>B. cereus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study identified a bacteriophage with a wide host range against\n                      <jats:italic>B. cereus<\/jats:italic>\n                      with potential applications in food storage and food safety.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.21857","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:40:23Z","timestamp":1792053623000},"page":"e21857","source":"Crossref","is-referenced-by-count":0,"title":["Application of bacteriophages in the prevention of\n                    <i>Bacillus cereus<\/i>\n                    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