{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:01:37Z","timestamp":1772269297530,"version":"3.50.1"},"reference-count":79,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008284","name":"University of New Hampshire","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008284","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Rodents of the genus\n                    <jats:italic>Peromyscus<\/jats:italic>\n                    have become increasingly utilized models for investigations into adaptive biology. This genus is particularly powerful for research linking genetics with adaptive physiology or behaviors, and recent research has capitalized on the unique opportunities afforded by the ecological diversity of these rodents. Well characterized genomic and transcriptomic data is intrinsic to explorations of the genetic architecture responsible for ecological adaptations. Therefore, this study characterizes the transcriptome of three male reproductive tissues (testes, epididymis and vas deferens) of\n                    <jats:italic>Peromyscus eremicus<\/jats:italic>\n                    (Cactus mouse), a desert specialist. The transcriptome assembly process was optimized in order to produce a high quality and substantially complete annotated transcriptome. This composite transcriptome was generated to characterize the expressed transcripts in the male reproductive tract of\n                    <jats:italic>P. eremicus,<\/jats:italic>\n                    which will serve as a crucial resource for future research investigating our hypothesis that the male Cactus mouse possesses an adaptive reproductive phenotype to mitigate water-loss from ejaculate. This study reports genes under positive selection in the male Cactus mouse reproductive transcriptome relative to transcriptomes from\n                    <jats:italic>Peromyscus maniculatus<\/jats:italic>\n                    (deer mouse) and\n                    <jats:italic>Mus musculus.<\/jats:italic>\n                    Thus, this study expands upon existing genetic research in this species, and we provide a high quality transcriptome to enable further explorations of our proposed hypothesis for male Cactus mouse reproductive adaptations to minimize seminal fluid loss.\n                  <\/jats:p>","DOI":"10.7717\/peerj.2617","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T04:40:51Z","timestamp":1477543251000},"page":"e2617","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of a male reproductive transcriptome for\n                    <i>Peromyscus eremicus<\/i>\n                    (Cactus mouse)"],"prefix":"10.7717","volume":"4","author":[{"given":"Lauren L.","family":"Kordonowy","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular, and Biological Sciences, University of New Hampshire, Durham, NH, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew D.","family":"MacManes","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Biological Sciences, University of New Hampshire, Durham, NH, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"issue":"3","key":"10.7717\/peerj.2617\/ref-1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"Journal of Molecular 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