{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:05:51Z","timestamp":1792476351347,"version":"4.5.0"},"reference-count":55,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["Project NO. 31702147"],"award-info":[{"award-number":["Project NO. 31702147"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006606","name":"Tianjin Natural Science Foundation","doi-asserted-by":"crossref","award":["Project NO. 18JCYBJC30000"],"award-info":[{"award-number":["Project NO. 18JCYBJC30000"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"\n Lactobacillus<\/jats:italic>\n supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with\n Lactobacillus acidophilus<\/jats:italic>\n on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1%\n L.\u00a0acidophilus<\/jats:italic>\n , for 14 days. As a result, dietary\n L. acidophilus<\/jats:italic>\n supplementation was found to increase the average daily gain (ADG) (\n P<\/jats:italic>\n \u00a0<\u00a00.05), reduced serum diamine oxidase (DAO) activity (\n P<\/jats:italic>\n \u00a0<\u00a00.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (\n P<\/jats:italic>\n \u00a0<\u00a00.01), reduced the mRNA levels of NOD1 (\n P<\/jats:italic>\n \u00a0<\u00a00.01), receptor interacting serine\/threonine kinase 2 (RIPK2) (\n P<\/jats:italic>\n \u00a0<\u00a00.05), nuclear factor \u03baB (NF-\u03baB) (\n P<\/jats:italic>\n \u00a0<\u00a00.01), NLR family pyrin domain containing 3 (NLRP3) (\n P<\/jats:italic>\n \u00a0<\u00a00.01), caspase-1 (\n P<\/jats:italic>\n \u00a0<\u00a00.01), interleukin 1\u03b2 (IL-1\u03b2) (\n P<\/jats:italic>\n \u00a0<\u00a00.05) and IL-18 (\n P<\/jats:italic>\n \u00a0<\u00a00.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (\n P<\/jats:italic>\n \u00a0<\u00a00.05), caspase-1 (\n P<\/jats:italic>\n \u00a0<\u00a00.01), IL-1\u03b2 (\n P<\/jats:italic>\n \u00a0<\u00a00.05) and IL-18 (\n P<\/jats:italic>\n \u00a0<\u00a00.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that\n L. acidophilus<\/jats:italic>\n supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that\n L. acidophilus<\/jats:italic>\n enhances the intestinal physical barrier functioning by inhibiting IL-1\u03b2 and IL-18 pro-inflammatory cytokines via the NOD1\/NLRP3 signaling pathway in weaned piglets.\n <\/jats:p>","DOI":"10.7717\/peerj.6060","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T03:51:17Z","timestamp":1545105077000},"page":"e6060","source":"Crossref","is-referenced-by-count":17,"title":["Effects of dietary supplementation with\n Lactobacillus acidophilus<\/i>\n on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets"],"prefix":"10.7717","volume":"6","author":[{"given":"Shiqiong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longbin","family":"Chen","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 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