{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:14:56Z","timestamp":1772270096885,"version":"3.50.1"},"reference-count":65,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Biotechnology, India","award":["No. BT\/PR13546\/BRB\/10\/1423\/2015"],"award-info":[{"award-number":["No. BT\/PR13546\/BRB\/10\/1423\/2015"]}]},{"name":"Government of India, Ministry of Human Resource Development"},{"DOI":"10.13039\/501100005808","name":"Indian Institute of Technology Bombay","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005808","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    harbors two endosymbiotic organelles: a relict plastid, the apicoplast, and a mitochondrion. The parasite expresses an antioxidant protein, thioredoxin peroxidase 1\/2 (TgTPx1\/2), that is dually targeted to these organelles. Nuclear-encoded proteins such as TgTPx1\/2 are trafficked to the apicoplast via a secretory route through the endoplasmic reticulum (ER) and to the mitochondrion via a non-secretory pathway comprising of translocon uptake. Given the two distinct trafficking pathways for localization to the two organelles, the signals in TgTPx1\/2 for this dual targeting are open areas of investigation. Here we show that the signals for apicoplast and mitochondrial trafficking lie in the N-terminal 50 amino acids of the protein and are overlapping. Interestingly, mutational analysis of the overlapping stretch shows that despite this overlap, the signals for individual organellar uptake can be easily separated. Further, deletions in the N-terminus also reveal a 10 amino acid stretch that is responsible for targeting the protein from punctate structures surrounding the apicoplast into the organelle itself. Collectively, results presented in this report suggest that an ambiguous signal sequence for organellar uptake combined with a hierarchy of recognition by the protein trafficking machinery drives the dual targeting of TgTPx1\/2.\n                  <\/jats:p>","DOI":"10.7717\/peerj.7215","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T04:00:53Z","timestamp":1563422453000},"page":"e7215","source":"Crossref","is-referenced-by-count":5,"title":["An ambiguous N-terminus drives the dual targeting of an antioxidant protein Thioredoxin peroxidase (TgTPx1\/2) to endosymbiotic organelles in\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.7717","volume":"7","author":[{"given":"Pragati","family":"Mastud","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swati","family":"Patankar","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4443","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"10.7717\/peerj.7215\/ref-1","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1093\/mp\/ssr092","article-title":"Dual targeting of a processing peptidase into both endosymbiotic organelles mediated by a transport signal of unusual architecture","volume":"5","author":"Baudisch","year":"2012","journal-title":"Molecular Plant"},{"key":"10.7717\/peerj.7215\/ref-2","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1111\/nph.12482","article-title":"The exception proves the rule? 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