{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:15:00Z","timestamp":1772270100379,"version":"3.50.1"},"reference-count":62,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad de Antioquia UdeA, and by a Ph.D. Studentship funded by Colciencias","award":["11551929249"],"award-info":[{"award-number":["11551929249"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The human parasite\n                      <jats:italic>Leishmania (V.) panamensis<\/jats:italic>\n                      is one of the pathogenic species responsible for cutaneous leishmaniasis in Central and South America. Despite its importance in molecular parasitology, its mitochondrial genome, divided into minicircles and maxicircles, haven\u2019t been described so far.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using NGS-based sequencing (454 and ILLUMINA), and combining\n                      <jats:italic>de novo<\/jats:italic>\n                      genome assembly and mapping strategies, we report the maxicircle kDNA annotated genome of\n                      <jats:italic>L. (V.) panamensis<\/jats:italic>\n                      , the first reference of this molecule for the subgenus\n                      <jats:italic>Viannia<\/jats:italic>\n                      . A comparative genomics approach is performed against other\n                      <jats:italic>Leishmania<\/jats:italic>\n                      and\n                      <jats:italic>Trypanosoma<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results show synteny of mitochondrial genes of\n                      <jats:italic>L. (V.) panamensis<\/jats:italic>\n                      with other kinetoplastids. It was also possible to identify nucleotide variants within the coding regions of the maxicircle, shared among some of them and others specific to each strain. Furthermore, we compared the minicircles kDNA sequences of two strains and the results show that the conserved and divergent regions of the minicircles exhibit strain-specific associations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.7717\/peerj.7235","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T03:43:31Z","timestamp":1562039011000},"page":"e7235","source":"Crossref","is-referenced-by-count":13,"title":["Mitochondrial genomics of human pathogenic parasite\n                    <i>Leishmania<\/i>\n                    (\n                    <i>Viannia<\/i>\n                    )\n                    <i>panamensis<\/i>"],"prefix":"10.7717","volume":"7","author":[{"given":"Daniel Alfonso","family":"Urrea","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaciones en Parasitolog\u00eda Tropical (LIPT), Departamento de Biolog\u00eda, Facultad de Ciencias, Universidad del Tolima, Ibague, Tolima, Colombia"},{"name":"Grupo Biolog\u00eda y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medell\u00edn, Antioquia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar","family":"Triana-Chavez","sequence":"additional","affiliation":[{"name":"Grupo Biolog\u00eda y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medell\u00edn, Antioquia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan F.","family":"Alzate","sequence":"additional","affiliation":[{"name":"Centro Nacional de Secuenciaci\u00f3n Gen\u00f3mica -CNSG, Sede de Investigaci\u00f3n Universitaria -SIU. 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