{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:14:04Z","timestamp":1790428444903,"version":"4.1.0"},"reference-count":71,"publisher":"PeerJ","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Government Research Training Program scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Bacteria capable of dechlorinating the toxic environmental contaminant dichloromethane (DCM, CH\n                    <jats:sub>2<\/jats:sub>\n                    Cl\n                    <jats:sub>2<\/jats:sub>\n                    ) are of great interest for potential bioremediation applications. A novel, strictly anaerobic, DCM-fermenting bacterium, \u201cDCMF\u201d, was enriched from organochlorine-contaminated groundwater near Botany Bay, Australia. The enrichment culture was maintained in minimal, mineral salt medium amended with dichloromethane as the sole energy source. PacBio whole genome SMRT\n                    <jats:sup>TM<\/jats:sup>\n                    sequencing of DCMF allowed\n                    <jats:italic>de novo<\/jats:italic>\n                    , gap-free assembly despite the presence of cohabiting organisms in the culture. Illumina sequencing reads were utilised to correct minor indels. The single, circularised 6.44 Mb chromosome was annotated with the IMG pipeline and contains 5,773 predicted protein-coding genes. Based on 16S rRNA gene and predicted proteome phylogeny, the organism appears to be a novel member of the\n                    <jats:italic>Peptococcaceae<\/jats:italic>\n                    family. The DCMF genome is large in comparison to known DCM-fermenting bacteria. It includes an abundance of methyltransferases, which may provide clues to the basis of its DCM metabolism, as well as potential to metabolise additional methylated substrates such as quaternary amines. Full annotation has been provided in a custom genome browser and search tool, in addition to multiple sequence alignments and phylogenetic trees for every predicted protein,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.slimsuite.unsw.edu.au\/research\/dcmf\/\">http:\/\/www.slimsuite.unsw.edu.au\/research\/dcmf\/<\/jats:ext-link>\n                    .\n                  <\/jats:p>","DOI":"10.7717\/peerj.7775","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T03:57:54Z","timestamp":1569988674000},"page":"e7775","source":"Crossref","is-referenced-by-count":18,"title":["Whole genome sequencing of a novel, dichloromethane-fermenting\n                    <i>Peptococcaceae<\/i>\n                    from an enrichment culture"],"prefix":"10.7717","volume":"7","author":[{"given":"Sophie I.","family":"Holland","sequence":"first","affiliation":[{"name":"UNSW Water Research 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Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonia L.","family":"Russell","sequence":"additional","affiliation":[{"name":"Ramaciotti Centre for Genomics, University of New South Wales, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandan P.","family":"Deshpande","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Manefield","sequence":"additional","affiliation":[{"name":"UNSW Water Research Centre, School of Civil and Environmental Engineering, University of New South Wales, Sydney, New South Wales, Australia"},{"name":"School of Chemical Engineering, University of New South Wales, Sydney, New South Wales, 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